동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제20권6호
  • /
  • Pages.1649-1653
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  • 2006
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

Effects of Hwangryun-Hae-Dok-tang on TNF-${alpha}$ and IL-4 Stimulated TARC, eotaxin, RANTES in the Human Bronchial Epithelial A549 Cells

  • Choi, Jong-Min (Department of Oriental Medical Ophthalmology & Otolaryngology & Dermatology, College of Oriental Medicine, Semyung University) ;
  • Kim, Hee-Taek (Department of Oriental Medical Ophthalmology & Otolaryngology & Dermatology, College of Oriental Medicine, Semyung University)
  • 발행 : 2006.12.25
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초록

Allergic diseases are the result of Th2-dominated responses to single or multiple environmental allergens. Th2 cytokines regulate these mechanisms of allergic disease at many levels, including initiation, progression, and persistence. The effect of hwangryun-Hae-Dok-Tang (HRHDT) on tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interleukin-4 (IL-4) stimulated inflammation was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay, thymus and activation-regulated chemokine (TARC), eotaxin, regulated on activation normal T cells expressed and secreted (RANTES) immunoassay on the human bronchial epithelial microglial cells. From the present study, the crude extract of Hwangryun-Hae-Dok-tang suppressed the TNF-${\alpha}$ and IL-4 stimulated TARC, eotaxin, and RANTES production in the human bronchial epithelial A549 cells. Based on the present results, Hwangryun-Hae-Dok-tang may be useful in the treatment asthmatic allergy by inhibiting TARC, eotaxin, and RANTES chemokines.

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참고문헌

  1. Romagnani, S. The role of lynphocytes in allergic disease. J. Allergy Clin. Immunol. 105:399-408, 2000 https://doi.org/10.1067/mai.2000.104575
  2. Romagnani, S. Cytokines and chemoattrants in allergic inflammation. Molecular Immunol. 38:881-885, 2001 https://doi.org/10.1016/S0161-5890(02)00013-5
  3. Bochner, B.S., Schleimer, R.P. The role of adhesion molecules in human eosinophils and basophils recruitment. J. Allergy Clin. Immunol. 94:427-438, 1994 https://doi.org/10.1016/0091-6749(94)90195-3
  4. Broide, D.H., Lots, M., Cuomo, A.J., Coburn, D.A., Federman, E.C., Wasserman, S.I. Cytokines in symptomatic asthma airways. J. Allergy Clin. Immunol. 89:958-967, 1992 https://doi.org/10.1016/0091-6749(92)90218-Q
  5. Tonnel, A.B., Gosset, P., Molet, S., Tillie-Leblond, I., Jeannin, P., Joseph, M. Interactions between endothelial cells and effector cells in allergic inflammation. Ann. N. Y. Acad. Sci. 796:9-20, 1996 https://doi.org/10.1111/j.1749-6632.1996.tb32562.x
  6. Chae, S.C., Lee, Y.C., Park, Y.R., Shin, J.S., Song, J.H., Oh, G.J., Hong, S.T., Pae, H.O., Choi, B.M., Chung, H.T. Analysis of the polymorphisms in eotaxin gene family and their association with asthma, IgE and eosinophil. Biochem. Biophys. Res. Commun. 320:131-137, 2004 https://doi.org/10.1016/j.bbrc.2004.05.136
  7. Berin, M.C. The Role of TARC in the pathogenesis of allergic asthma. Drug News Perspect. 15:10-16, 2002 https://doi.org/10.1358/dnp.2002.15.1.660501
  8. Hein, H., Schluter, C., Kulke, R., Christophers, E., Schroder, J.M., Bartels, J. Genomic organisation, sequence, and transcriptional regulation of the human eotaxin gene. Biochem. Biophys. Res. Commun. 237:537-542, 1997 https://doi.org/10.1006/bbrc.1997.7169
  9. Ieki, K., Matsukura, S., Kokubu, F., Kimura, T., Kuga, H., Kawaguchi, M., Odaka, M., Suzuki, S., Watanabe, S., Takeuchi, H., Schleimer, R.P., Adachi, M. Double-stranded RNA activates RANTES gene transcription through co-operation of nuclear factor-kappaB and interferon regulatory factors in human airway epithelial cells. Clin. Exp. Allergy. 34:745-752, 2004 https://doi.org/10.1111/j.1365-2222.2004.1941.x
  10. Ohta, Y., Kobayashi, T., Nishida, K., Sasaki, E., Ishiguro, I. Preventive effect of Oren-gedoku-to(Huanglian-Jie-Du-Tang) extract on the development of stress-induced acute gastric mucosal lesions in rats. J. Ethnopharmacol. 67:377-384, 1999 https://doi.org/10.1016/S0378-8741(99)00093-8
  11. Fujiwara, M., Iwasaki, K. Toki-shakuyaku-San and Oren-Gedoku-To improve the disruption of spatial cognition induced by cerebral ischemia and central cholinergic dysfunction in rats. Phytother. Res. 7:S60-S62, 1993 https://doi.org/10.1002/ptr.2650070718
  12. Wang, L.M., Mineshita, S. Preventive effects of unsei-in and oren-gedoku-to, Chinese traditional medicines, against rat paw oedema and abdominal constriction in mice. J. Pharm. Pharmacol. 48:327-331, 1996 https://doi.org/10.1111/j.2042-7158.1996.tb05927.x
  13. Sekiya, T., Miyamasu, M., Imanishi, M., Yamada, H., Nakajima, T., Yamaguchi, M., Fujisawa, T., Pawankar, R., Sano, Y., Ohta, K., Ishii, A., Morita, Y., Yamamoto, K., Matsushima, K., Yoshie, O., Hirai, K. Inducible expression of a Th2-type CC chemokine thymus-and activation-regulated chemokine by human bronchial epithelial cells. J. Immunol. 165:2205-2213, 2000 https://doi.org/10.4049/jimmunol.165.4.2205
  14. Ehrlich, L.C., Hu, S., Sheng, W.S., Sutton, R.L., Rockswold, G.L., Peterson, P.K., Chao, C.C. Cytokine regulation of human microglial cell IL-8 production. J. Immunol. 160:1944-1948, 1998
  15. Bruce, S., Bochner, M.D., William, W., Busse, M.D. Advances in mechanism of allergy. J. Allergy Clin. Immunol. 113:868-875, 2004 https://doi.org/10.1016/j.jaci.2004.02.016
  16. Dean, D.M., Dana, B., Yoseph, A.M. Mast cells. Physiological reviews J. 77:1052, 1997
  17. Bradding, P., I.H. Feather, P.H. Howarth, R. Mueller, J.A. Roberts, K. Britten, J. bews, T.C. Hunt, Y. Okayama, C.H. Heusser, G.R. Bullock, M.K. Church, S.T. Holgate. Interleukin 4 is localized to and released by human mast cells. J. Exp. Med. 176:1381-1386, 1992 https://doi.org/10.1084/jem.176.5.1381
  18. Bradding, P., I.H. Feather, S., Wilson, P.G. Bradin, C.H. Heusser, S.T. Holgate, P.H. Howarth. Immunolocalization of cytokines in the nasal mucosa of normal and perennial rhinitis subjects. J. Immunol. 151:3853-3865, 1993
  19. Moller, A., U. Pippert, D. Lessmann, G. Kolde, K. Hamann, P. Walker, D. Schadendorf, T. Rosenbach, T. Luger, B.M. Czarnitzki. Human mast cells produce IL-8. J. Immunonl. 151:3261-3266, 1993
  20. Okhawara, Y., K. Yamauchi, Y. Tanno. Human lung mast cells and pulmonary macrophages produce tumor necrosis factor-$\alpha$ in sensitized lung tissue after IgE receptor triggering. Am. J. Respir. Cell Mol. Biol. 7:385, 1992 https://doi.org/10.1165/ajrcmb/7.4.385
  21. Bradding P., Y. Okayama, P.H. Howarth, M.K. Church, S.T. Holgate. Heterogeneity of human mast cells based on cytokine content . J. Immunol. 155:297-307, 1995
  22. Selvan, R.S., J.H. Butterfield, M.S. Krangel. Expression of multiple cytokine genes by a human mast cell leukemia. J. Biol. Chem. 269:13893-13898, 1994
  23. Gavett, D.J., Karp, C.L., Patel, E.A., Schofield, B.H., Finkelman, F.D., Wills-Karp M. Interleukin-4 receptor blockade prevents airway responses induced by antigen challenge in mice. Am. J. Physiol. 272:L253-L261, 1997
  24. Yu, B., Koga, T., Urabe, K., Moroi, Y., Maeda, S., Yanagihara, Y., Furue, M. Differential regulation of thymus- and activation-regulated chemokine induced by IL-4, IL-13, TNF-alpha and IFN-gamma in human keratinocyte and fibroblast. J. Dermatol. Sci. 30:29-36, 2002 https://doi.org/10.1016/S0923-1811(02)00046-4
  25. Hoeck, J., Woisetschlager, M. STAT6 mediates eotaxin-1 expression IL-4 or TNF-$\alpha$-induced fibroblasts. J. Immunol. 166:4507-4515, 2001 https://doi.org/10.4049/jimmunol.166.7.4507
  26. Sabatini, F., Silvestri, M., Sale, R., Serpero, L., Raynal, M.E., Di Blasi, P., Rossi, G.A. Modulation of the constitutive or cytokine-induced bronchial epithelial cell functions in vitro by fluticasone propionate. Immunol. Lett. 89:215-224, 2003 https://doi.org/10.1016/S0165-2478(03)00142-1
  27. Sabatini, F., Silvestri, M., Sale, R., Scarso, L., Defilippi, A.C., Risso, F.M., Rossi, G.A. Fibroblast-eosinophil interaction modulation of adhesion molecules expression and chemokine release by human fetal lung fibroblasts in response to IL-4 and TNF-$\alpha$. Immunol. Lett. 84:173-178, 2002 https://doi.org/10.1016/S0165-2478(02)00183-9
  28. Bisset, L.R., Schmid-Grendelmeier, P. Chemokines and their receptors in the pathogenesis of allergic asthma: progress and perspective. Curr Opin Pulm Med. 11:35-42, 2005 https://doi.org/10.1097/01.mcp.0000144502.50149.e0
  29. Lin, C.C., Shieh, D.E. The anti-inflammatory activity of Scutellaria rivularis extracts and its active components, baicalin, baicalein and wogonin. Am. J Chin Med 24:31-36, 1996 https://doi.org/10.1142/S0192415X96000050
  30. Koda, A., Nishiyori, T., Nagai, H., Matsuura, N., Tsuchiya, H. Anti-allergic actions of traditional oriental medicine-actions against types I and IV hypersensitivity reactions. Nippon Yakurigaku Zasshi 80:31-41, 1982 https://doi.org/10.1254/fpj.80.31
  31. Fukutake, M., Miura, N., Yamamoto, M., Fukuda, K., Lijima, O., Ishikawa, H., Kubo, M., Okada, M., Komatsu, Y., Sasaki, H., Wakabayashi, K., Ishige, A., Amagaya, S. Suppressive effect of the herbal medicine Oren-gedoku-to on cyclooxygenase-2 activity and azoxymethane-induced aberrant crypt foci development in rats. Cancer Lett. 157:9-14, 2000 https://doi.org/10.1016/S0304-3835(00)00432-8