Browse > Article

Effects of Hwangryun-Hae-Dok-tang on TNF-${alpha}$ and IL-4 Stimulated TARC, eotaxin, RANTES in the Human Bronchial Epithelial A549 Cells  

Choi, Jong-Min (Department of Oriental Medical Ophthalmology & Otolaryngology & Dermatology, College of Oriental Medicine, Semyung University)
Kim, Hee-Taek (Department of Oriental Medical Ophthalmology & Otolaryngology & Dermatology, College of Oriental Medicine, Semyung University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.6, 2006 , pp. 1649-1653 More about this Journal
Abstract
Allergic diseases are the result of Th2-dominated responses to single or multiple environmental allergens. Th2 cytokines regulate these mechanisms of allergic disease at many levels, including initiation, progression, and persistence. The effect of hwangryun-Hae-Dok-Tang (HRHDT) on tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interleukin-4 (IL-4) stimulated inflammation was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay, thymus and activation-regulated chemokine (TARC), eotaxin, regulated on activation normal T cells expressed and secreted (RANTES) immunoassay on the human bronchial epithelial microglial cells. From the present study, the crude extract of Hwangryun-Hae-Dok-tang suppressed the TNF-${\alpha}$ and IL-4 stimulated TARC, eotaxin, and RANTES production in the human bronchial epithelial A549 cells. Based on the present results, Hwangryun-Hae-Dok-tang may be useful in the treatment asthmatic allergy by inhibiting TARC, eotaxin, and RANTES chemokines.
Keywords
Hwangyryun-Hae-Dok-tang; Allergy; TARC; Eotazin; RANTES;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ohta, Y., Kobayashi, T., Nishida, K., Sasaki, E., Ishiguro, I. Preventive effect of Oren-gedoku-to(Huanglian-Jie-Du-Tang) extract on the development of stress-induced acute gastric mucosal lesions in rats. J. Ethnopharmacol. 67:377-384, 1999   DOI   ScienceOn
2 Fujiwara, M., Iwasaki, K. Toki-shakuyaku-San and Oren-Gedoku-To improve the disruption of spatial cognition induced by cerebral ischemia and central cholinergic dysfunction in rats. Phytother. Res. 7:S60-S62, 1993   DOI   ScienceOn
3 Sekiya, T., Miyamasu, M., Imanishi, M., Yamada, H., Nakajima, T., Yamaguchi, M., Fujisawa, T., Pawankar, R., Sano, Y., Ohta, K., Ishii, A., Morita, Y., Yamamoto, K., Matsushima, K., Yoshie, O., Hirai, K. Inducible expression of a Th2-type CC chemokine thymus-and activation-regulated chemokine by human bronchial epithelial cells. J. Immunol. 165:2205-2213, 2000   DOI
4 Ehrlich, L.C., Hu, S., Sheng, W.S., Sutton, R.L., Rockswold, G.L., Peterson, P.K., Chao, C.C. Cytokine regulation of human microglial cell IL-8 production. J. Immunol. 160:1944-1948, 1998
5 Moller, A., U. Pippert, D. Lessmann, G. Kolde, K. Hamann, P. Walker, D. Schadendorf, T. Rosenbach, T. Luger, B.M. Czarnitzki. Human mast cells produce IL-8. J. Immunonl. 151:3261-3266, 1993
6 Selvan, R.S., J.H. Butterfield, M.S. Krangel. Expression of multiple cytokine genes by a human mast cell leukemia. J. Biol. Chem. 269:13893-13898, 1994
7 Sabatini, F., Silvestri, M., Sale, R., Scarso, L., Defilippi, A.C., Risso, F.M., Rossi, G.A. Fibroblast-eosinophil interaction modulation of adhesion molecules expression and chemokine release by human fetal lung fibroblasts in response to IL-4 and TNF-$\alpha$. Immunol. Lett. 84:173-178, 2002   DOI   ScienceOn
8 Bisset, L.R., Schmid-Grendelmeier, P. Chemokines and their receptors in the pathogenesis of allergic asthma: progress and perspective. Curr Opin Pulm Med. 11:35-42, 2005   DOI
9 Koda, A., Nishiyori, T., Nagai, H., Matsuura, N., Tsuchiya, H. Anti-allergic actions of traditional oriental medicine-actions against types I and IV hypersensitivity reactions. Nippon Yakurigaku Zasshi 80:31-41, 1982   DOI   ScienceOn
10 Lin, C.C., Shieh, D.E. The anti-inflammatory activity of Scutellaria rivularis extracts and its active components, baicalin, baicalein and wogonin. Am. J Chin Med 24:31-36, 1996   DOI   ScienceOn
11 Bradding, P., I.H. Feather, S., Wilson, P.G. Bradin, C.H. Heusser, S.T. Holgate, P.H. Howarth. Immunolocalization of cytokines in the nasal mucosa of normal and perennial rhinitis subjects. J. Immunol. 151:3853-3865, 1993
12 Romagnani, S. The role of lynphocytes in allergic disease. J. Allergy Clin. Immunol. 105:399-408, 2000   DOI   ScienceOn
13 Berin, M.C. The Role of TARC in the pathogenesis of allergic asthma. Drug News Perspect. 15:10-16, 2002   DOI   ScienceOn
14 Dean, D.M., Dana, B., Yoseph, A.M. Mast cells. Physiological reviews J. 77:1052, 1997
15 Chae, S.C., Lee, Y.C., Park, Y.R., Shin, J.S., Song, J.H., Oh, G.J., Hong, S.T., Pae, H.O., Choi, B.M., Chung, H.T. Analysis of the polymorphisms in eotaxin gene family and their association with asthma, IgE and eosinophil. Biochem. Biophys. Res. Commun. 320:131-137, 2004   DOI   ScienceOn
16 Hoeck, J., Woisetschlager, M. STAT6 mediates eotaxin-1 expression IL-4 or TNF-$\alpha$-induced fibroblasts. J. Immunol. 166:4507-4515, 2001   DOI
17 Tonnel, A.B., Gosset, P., Molet, S., Tillie-Leblond, I., Jeannin, P., Joseph, M. Interactions between endothelial cells and effector cells in allergic inflammation. Ann. N. Y. Acad. Sci. 796:9-20, 1996   DOI
18 Ieki, K., Matsukura, S., Kokubu, F., Kimura, T., Kuga, H., Kawaguchi, M., Odaka, M., Suzuki, S., Watanabe, S., Takeuchi, H., Schleimer, R.P., Adachi, M. Double-stranded RNA activates RANTES gene transcription through co-operation of nuclear factor-kappaB and interferon regulatory factors in human airway epithelial cells. Clin. Exp. Allergy. 34:745-752, 2004   DOI   ScienceOn
19 Okhawara, Y., K. Yamauchi, Y. Tanno. Human lung mast cells and pulmonary macrophages produce tumor necrosis factor-$\alpha$ in sensitized lung tissue after IgE receptor triggering. Am. J. Respir. Cell Mol. Biol. 7:385, 1992   DOI   ScienceOn
20 Wang, L.M., Mineshita, S. Preventive effects of unsei-in and oren-gedoku-to, Chinese traditional medicines, against rat paw oedema and abdominal constriction in mice. J. Pharm. Pharmacol. 48:327-331, 1996   DOI   ScienceOn
21 Romagnani, S. Cytokines and chemoattrants in allergic inflammation. Molecular Immunol. 38:881-885, 2001   DOI   ScienceOn
22 Fukutake, M., Miura, N., Yamamoto, M., Fukuda, K., Lijima, O., Ishikawa, H., Kubo, M., Okada, M., Komatsu, Y., Sasaki, H., Wakabayashi, K., Ishige, A., Amagaya, S. Suppressive effect of the herbal medicine Oren-gedoku-to on cyclooxygenase-2 activity and azoxymethane-induced aberrant crypt foci development in rats. Cancer Lett. 157:9-14, 2000   DOI   ScienceOn
23 Broide, D.H., Lots, M., Cuomo, A.J., Coburn, D.A., Federman, E.C., Wasserman, S.I. Cytokines in symptomatic asthma airways. J. Allergy Clin. Immunol. 89:958-967, 1992   DOI
24 Yu, B., Koga, T., Urabe, K., Moroi, Y., Maeda, S., Yanagihara, Y., Furue, M. Differential regulation of thymus- and activation-regulated chemokine induced by IL-4, IL-13, TNF-alpha and IFN-gamma in human keratinocyte and fibroblast. J. Dermatol. Sci. 30:29-36, 2002   DOI
25 Bradding P., Y. Okayama, P.H. Howarth, M.K. Church, S.T. Holgate. Heterogeneity of human mast cells based on cytokine content . J. Immunol. 155:297-307, 1995
26 Bruce, S., Bochner, M.D., William, W., Busse, M.D. Advances in mechanism of allergy. J. Allergy Clin. Immunol. 113:868-875, 2004   DOI   ScienceOn
27 Bradding, P., I.H. Feather, P.H. Howarth, R. Mueller, J.A. Roberts, K. Britten, J. bews, T.C. Hunt, Y. Okayama, C.H. Heusser, G.R. Bullock, M.K. Church, S.T. Holgate. Interleukin 4 is localized to and released by human mast cells. J. Exp. Med. 176:1381-1386, 1992   DOI   ScienceOn
28 Bochner, B.S., Schleimer, R.P. The role of adhesion molecules in human eosinophils and basophils recruitment. J. Allergy Clin. Immunol. 94:427-438, 1994   DOI   ScienceOn
29 Gavett, D.J., Karp, C.L., Patel, E.A., Schofield, B.H., Finkelman, F.D., Wills-Karp M. Interleukin-4 receptor blockade prevents airway responses induced by antigen challenge in mice. Am. J. Physiol. 272:L253-L261, 1997
30 Sabatini, F., Silvestri, M., Sale, R., Serpero, L., Raynal, M.E., Di Blasi, P., Rossi, G.A. Modulation of the constitutive or cytokine-induced bronchial epithelial cell functions in vitro by fluticasone propionate. Immunol. Lett. 89:215-224, 2003   DOI
31 Hein, H., Schluter, C., Kulke, R., Christophers, E., Schroder, J.M., Bartels, J. Genomic organisation, sequence, and transcriptional regulation of the human eotaxin gene. Biochem. Biophys. Res. Commun. 237:537-542, 1997   DOI   ScienceOn