Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Danchunhwan Protects the Cytotoxicity of Beta-amyloid in SH-SY5Y Neuroblastoma Cells

베타아밀로이드 유도성 SH-SY5Y 세포독성에서 단천환(丹川丸)의 보호효과

  • Yu, Bong-Sun (Department of internal Medicine School of Oriental Medicine) ;
  • Kim, Jin-Kyung (Department of Microbiology School of Medicine, Wonkwang University) ;
  • ;
  • Park, Chan-Ny (Department of Microbiology School of Medicine, Wonkwang University) ;
  • So, Hong-Seob (Department of Microbiology School of Medicine, Wonkwang University) ;
  • 유봉선 (원광대학교 한의과대학 심계내과학교실) ;
  • 김진경 (원광대학교 의과대학 미생물학교실) ;
  • 남상규 (원광대학교 한의과대학 심계내과학교실) ;
  • 박찬희 (원광대학교 의과대학 미생물학교실) ;
  • 소홍섭 (원광대학교 의과대학 미생물학교실) ;
  • Published : 2006.12.25
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Abstract

The water extract of Danchunhwan(DCH) has been traditionally used for treatment of dementia damage in oriental medicine. However, little is known about the mechanism by which the water extract of DCH rescues cells from neurodegenerative disease such as Alzheimer's disease. This study was designed to investigate the protective mechanisms of DCH on ${\beta}$-amyloid or $H_2O_2$-induced cytotoxicity in SH-SY5Y neuronblastoma cells. ${\beta}$-amyloid and $H_2O_2$ markedly decreased the viability of SH-SY5Y cells, which was characterized with apparent apoptotic features such as membrane blebbing as well as fragmentation of genomic DNA and nuclei. However, the water extract of DCH significantly reduced both ${\beta}$-amyloid or $H_2O_2$-induced cell death and apoptotic characteristics through reduction of intracellular peroxide generation. Also, the water extract of DCH prevented prevented the mitochondrial dysfunction including the disruption of mitochondria membrane permeability transition (MPT) and the perturbation in Bcl-2 family protein expressions in $H_2O_2$-treated SH-SY5Y cells.

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References

  1. 吳普. 神農本草經. 서울, 醫聖堂 p 24, 25, 29, 30, 1994
  2. 辛民敎. 臨床本草學. 서울, 永林社, pp 519-521, 530-532, 1997
  3. 鄭普燮, 辛民敎. 鄕藥(生藥)大辭典. 서울, 永林社, pp 418-419, 860-862, 1990
  4. 申佶求. 申氏本草學. 서울, 壽文社, pp 519-521, 600-602, 1988
  5. 李尙仁. 本草學. 서울, 醫藥社, pp 398-400,419-420, 1975
  6. 楊東喜. 本草備要解析. 서울, 醫聖堂, pp 29-30, 125-127, 1993
  7. 李時珍. 本草綱目. 北京, 人民衛生出版社, pp 758-760, 837-841, 1982
  8. 金晸壽. 標準本草學. 서울, 進明出版社, pp 107-108, 367-370, 1975
  9. 中華人民共和國衛生部藥典委員會. 中華人民共和國藥典. 北京, 人民衛生出版社, p 26, 58, 59, 1985
  10. 李尙仁 外. 漢藥臨床應用. 서울, 醫聖堂, pp 299-303, 1982
  11. 陳嘉謨. 本草蒙筌. 北京, 人民衛生出版社, pp 77-78, 119-120, 1988. / 金昌謙. 本草從新, 서울, 행림출판, pp19-20, 31, 1989
  12. 과학백과사전출판사. 실용동의약학. 서울, 일월서각, pp 161-163, 177-179, 1990
  13. 上海中醫學院. 中草藥學, 香港, 商務印書館香港分館, pp 119-127, 223-236. 1983
  14. 김창민, 辛民敎, 이경순, 안덕균 역. 中藥大辭典. 서울, 도서출판 정담, pp 1117-1126, 5258-5265, 1998
  15. 서순규. 成人病․老人醫學. 서울, 高麗醫學, pp 225-228, 230-232, 1992
  16. 大韓神經精神醫學會. 神經精神科學. 서울, 하나의학사, pp 211-220, 1997
  17. 黃義完 外. 東醫精神醫學. 서울, 現代醫學書籍社, pp 256-271, 327-330, 1992
  18. Reznik-Wolf, H., Machado, J., Haroutuian, V., DeMacro, L., Walter, G.F., Goldman, B., Davidson, M., Johnston, J.A., Lannfelt, L., Dani, S.U., Friedman, E. Somatic mutation analysis of the APP and Presenilin 1 and 2 genes in Alzheimer's disease brain. J Neurogenet. 12(1):55-65, 1998 https://doi.org/10.3109/01677069809108555
  19. Alzheimer's disease collaborative group. The structure of the presenilin 1(SI82) gene and identification of six novel mutation in early onset AD families. Nat Genet. 11(2):219-222, 1995 https://doi.org/10.1038/ng1095-219
  20. Tabaton, M., Cammarata, S., Mandybur, T., Richy, P., Kawai, M., Perry, G., Gambetti, P. Senile plaques in cerebral amyloid angiopathy show accumulation of amyloid precursor protein without cytoskeletal abnormalities. Brain Res. 593(2):299-303, 1992 https://doi.org/10.1016/0006-8993(92)91323-7
  21. 柳泳秀 외. 記憶障碍에 관한 東西醫學的 比較 硏究. 東醫神經精神科學會誌 7(1):155-166, 1996
  22. 崔龍埈. 健忘의 辨證分型에 대한 硏究. 大韓韓醫學會誌 17(1):374-406, 1996
  23. 金利和 외. 痴呆治療의 最近 硏究動向에 관한 考察. 大韓針灸學會誌 14(2):115-126, 1997
  24. 우주영. 調胃升淸湯이 흰쥐의 방사형 미로 학습과 기억에 미치는 영향. 東醫神經精神科學會誌, 8(1):69-79, 1997
  25. 金聖賢. 洗心湯이 腦組織의 酸化作用에 미치는 影響. 東醫神經精神科學會誌, 8(2): 39-50, 1997
  26. 黃始榮. 遠志에 의한 腦 星狀細胞로부터 炎症性 細胞活性物質 分泌의 抑制 效果에 關한 硏究. 東醫神經精神科學會誌 10(1):95-108, 1999
  27. 王浴生 : 中藥藥理與應用, 北京, 人民衛生出版社, pp119-127, 223-236, 1983
  28. 新文豊出版公司 編 : 新編中藥大辭典, 臺北, 新文豊出版公司, pp261-264, 302-306, 1982
  29. 한상혁. 단천환이 사람신경세포의 산화적 손상에 미치는 영향. 익산, 원광대학교 한의학전문대학원, 1999
  30. 나영훈. 단천환이 Hydrogen Peroxide에 의한 심근세포 독성에 미치는 영향. 익산, 원광대학교 한의학전문대학원, 2001
  31. 이근후. 최신임상정신의학. 서울, 하나의학사, pp 138, 216-228, 1988
  32. Lemos, F.B., Ijzermans, J.N., Zondervan, P.E., Peeters, A.M., Van Den Engel, S., Mol, W.M., Weimar, W., Baan, C.C. Differential expression of heme oxygenase-1 and vascular endothelial growth factor in cadaveric and living donor kidneys after ischemia-reperfusion. J Am Soc Nephrol. 14(12):3278-3287, 2003 https://doi.org/10.1097/01.ASN.0000098683.92538.66
  33. Gross, A., McDonnell, J.M., Korsmeyer, S.J. Bcl-2 family members and the mitochondria in apoptosis. Genes Dev. 13:1899-1911, 1999. Vander Heiden, M.G., and Thompson, C.B. Bcl-2 proteins: inhibitors of apoptosis or regulators of mitochondrial homeostasis? Nat Cell Biol. 1:E209-E216, 1999 https://doi.org/10.1038/70237
  34. Kroemer, G., Dallaporta, B., Resche-rigon, M. The mitochondrial death/life regulator in apoptosis and necrosis. Nature Med. 6: 614-620, 1998
  35. Reed, J.C. Bcl2 and the regulation of programmed cell death. J. Cell. Biol. 124: 1-6, 1994 https://doi.org/10.1083/jcb.124.1.1
  36. Herr, I., Wilhelm, D., Bohler, T., Angel, P., and Debatin, K. M. Activation of CD95 (APO-1/Fas) signaling by ceramide mediates cancer therapy-induced apoptosis. EMBO. 16: 6200-6208, 1997. https://doi.org/10.1093/emboj/16.20.6200
  37. LiPF, Dietz, R., Von Harsdorf, R. P53 regulates mitochondrial membrane potential through reactive oxygen species and induces cytochrome c-independent apoptosis blocked by Bcl-2. EMBO. 18: 6027-6036, 1999 https://doi.org/10.1093/emboj/18.21.6027
  38. Heibein, J.A., Barry, M., Motyka, B., Bleackley, R.C. Granzyme B-induced loss of mitochondrial inner membrane potential (Delta Psi m) and cytochrome c release are caspase independent. J Immunology. 163(9):4683-4693, 1999