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대파(Allium fistulosum L)로부터 fistulosides의 분리와 분리 물질의 항진균 활성

Antifungal Activity of Fistulosides, Steroidal Saponins, from Allium fistulosum L

  • 손호용 (안동대학교 식품영양학과) ;
  • 금은주 (안동대학교 식품영양학과) ;
  • 류희영 (안동대학교 식품영양학과) ;
  • 전수진 (안동대학교 식품영양학과) ;
  • 김남순 (한국생명공학연구원 기능유전체연구센터) ;
  • 손건호 (안동대학교 식품영양학과)
  • Sohn Ho-Yong (Department of Food and Nutrition, Andong National University) ;
  • Kum Eun-Joo (Department of Food and Nutrition, Andong National University) ;
  • Ryu Hee-Young (Department of Food and Nutrition, Andong National University) ;
  • Jeon Su-Jin (Department of Food and Nutrition, Andong National University) ;
  • Kim Nam-Soon (Genome Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Son Kun-Ho (Department of Food and Nutrition, Andong National University)
  • 발행 : 2006.04.01

초록

대파(Allium fistulosum L)는 백합과 다년초로서 한국, 일본 중국 등 전 세계적으로 식용으로 재배되어 왔다. 현재까지 대파의 다양한 생리활성이 보고되어 왔으나, 항균 활성에 대해서는 거의 보고되어 있지 않다. 본 연구에서는 대파 식용부위로부터 강력한 항미생물성 steroidal saponins (fistuloside A, B, 및 C)을 분리하였고, 이들의 항균력을 병원성균 및 식품부패균을 대상으로 평가하였다. Fistuloside A 및 C는 강력한 항진균 활성과 항Proteus 세균활성을 나타낸 반면, fistuloside B는 항진균 활성만 인정되었다. 특히 fistuloside C는 $3.1{\sim}6.2{\mu}g/ml$의 MIC (minimal inhibitory concentration) 및 MFC (minimal fungicidal concentration)를 나타내어 강력한 항진균 활성이 진균사멸에 의한 항균효과임을 확인하였다. 본 연구결과는 대파가 미생물 감염증 제어용으로 사용되어 온 근거를 제시하며, 또한 fistuloside C의 항진균제로서의 이용 가능성을 제시하고 있다.

Allium fistulosum L. (Welsh onion) is a perennial herb that is widely cultivated throughout the world, especially in China, Japan and Korea. Although, various activities were reported, little is known about antimicrobial activity of A. fistulosum L.. In this study, strong antimicrobial substances, fistuloside A, B, and C were isolated from the edible parts of A. fistulosum L. and their antimicrobial activity was evaluated with pathogenic- or food-spoilage microorganism based on disk-diffusion assay, minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) determination. Fistuloside A and fistuloside C showed strong antifungal activity and anti-Proteus activity, while fistuloside B is effective to only fungi. The fistuloside C showed a prominent antifungal activity with $3.1{\sim}6.2{\mu}g/ml$ of MIC and MFC. Our results indicated that fistuloside C has a prominent antifungal activity and support the use of A. fistulosum to treat microbial infection.

키워드

참고문헌

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피인용 문헌

  1. Steroidal saponins from the genus Allium vol.15, pp.1, 2016, https://doi.org/10.1007/s11101-014-9381-1
  2. Antifungal Activities of Commercial Rice Wine Extracts of Taiwanese <i>Allium fistulosum</i> vol.06, pp.07, 2016, https://doi.org/10.4236/aim.2016.67046
  3. Antibacterial, Antioxidative and Anti-proliferative Activity against Human Colorectal Cell of Pimpinella brachycarpa vol.18, pp.4, 2011, https://doi.org/10.11002/kjfp.2011.18.4.590
  4. Isolation and identification of cinnamic acid amides as antioxidants from Allium fistulosum L. and their free radical scavenging activity vol.20, pp.2, 2011, https://doi.org/10.1007/s10068-011-0078-6
  5. Antioxidant and Antimicrobial Activities of Ethanol Extract from Six Vegetables Containing Different Sulfur Compounds vol.41, pp.5, 2012, https://doi.org/10.3746/jkfn.2012.41.5.577