DOI QR코드

DOI QR Code

Selenium이 MPTP(1-methy-4-phenyl-1,2,3,6-tetrahydropyridine)에 의해 유도된 생쥐의 신경독성에 미치는 영향

Effect of Selenium Yeast on MPTP (1-methyl-4-phenyl-propion-oxypiperidine)-Induced Neurotoxicity in Mice

  • 발행 : 2006.04.01

초록

MPTP에 의해 유도된 Parkinsonism에 대한 selenium의 보호효과와 그 보호작용에 대한 항산화적 해독기전을 조사하기 위하여 MPTP 10mg/kg을 6일간 주사하고 selenium (25, 50, 100 ${\mu}g/kg$)을 10일간 경구 투여하였으며 처음 6일간은 selenium와 MPTP를 병용 투여하였다. 실험동물을 마지막으로 selenium을 투여하고 24시간 후에 치사시켜 일반적인 독성과 항산화 방어능과 관련된 지표성분과 monoamine oxidase와 같은 신경생화학적인 지표성분들을 뇌조직에서 측정하였으며 그 결과 다음과 같은 결론을 얻었다. 우선 MPTP를 투여함에 따라 운동능력이 저하되던 것이 selenium을 투여함에 따라 운동능력이 증가되었으며, 이러한 결과의 기전은 selelnium을 투여함으로써 MPTP를 $MPP^+$로 대사시키는 MAO-B의 활성을 억제하였으며 $MPP^+$에 의해 유도된 신경독성에 대한 selenium의 보호 효과는 selenium을 투여함으로써 활성산소 해독계인 SOD, catalase, glutathione peroxidase의 활성을 증가시키기 때문인 것으로 사료된다.

This study is investigated the effect of selenium against neurotoxicity induced by MPTP(1-methy-4-phenyl-propion-oxypiperidine) in mice. In order to demonstrate neuroprotective activity of selenium, mice were administrated orally with selenium(25, 50, 100 ${\mu}g/kg$, once/day) for 10 days, and MPTP(10 mg/kg) was injected subcutaneously into the mice for 6 days from the beginning 1hr before selenium treatment. Test of rota road activity was inhibited by treatment with selenium in MPTP-induced neurotoxicity group when compared to MPTP treatment group in normal mice. Monoamine oxidase(MAO)-B activity and cerebral lipid peroxide content were significantly decreased in the treatment of selenium in MPTP-induced neurotoxicity group when compared to MPTP treatment group in normal mice and MAO-A was not affected. Activities of cerebral superoxide dismutase, catalase and glutathione peroxidase were significantly increased in the treatment of selenium in MPTP-induced neurotoxicity group when compared to MPTP treatment group in normal mice. These results suggest that selenium might be estimated the result from the cooperative action of its inhibitory effect on monoamine oxidase-B with that of the enhancement of antioxidant(SOD, catalase, GSH-Px) defence ability.

키워드

참고문헌

  1. Adams, J. D., J. H. M. Johanessen, J. P. Schuller, S. P. Bacon and S. P. Markey. 1986. The role of oxidative stress in the systematic toxicity of MPTP and $MPP^+$ . pp. 571-571, Academic Press, New York
  2. Adams, J. D., L. K. Klaidman and A. C. Leugn. 1993. $MPP^+$ and $MPDP^+$ induced oxygen radical formation with mitochondrial enzymes. Free Radic. Biol. Med. 15, 181-186 https://doi.org/10.1016/0891-5849(93)90057-2
  3. Aebi, H. 1974. Catalase. In 'Methods of enzymetic analysis' Bergmeyer, H.U., Academic Press, New York., 2, 673-684
  4. Ballard, P. A., J, W. Tetrud and J. W. Lanston. 1985. Permanent human parkinsonism due to N-methyl-4-phenyl -1,2,3,6-tetrahydropyridine (MPTP). Neuro. 35, 949-956 https://doi.org/10.1212/WNL.35.7.949
  5. Bang, J. S. and S, J. Rhee. 1991 Effect of dietary selenium on ${\delta}-aminolevulinic$ aid dehydratase activity in lead poisoned rats. Korean J. Nutr. 24, 526-533
  6. Brady, P. S., J. E. Shelle and D. E. Elrey, 1977. Rapid changes in equine erythrocyte glutathione reductase with exercise. Am. J. Vet. Res. 38, 1045-1047
  7. Birkmayer, W., P. Riederer and W. D. Rausch. 1979. In advances in neurology(Poirier, L. H., Sourkes, T. L., Bedard, J. P., Eds). pp. 499-510. Ravan Press, New York
  8. Chan, P., L. E. Delanney, L. Irwin, J. W. Langston and D. D. Monte. 1991. Rapid ATP loss caused by N-methyl- 4-phenyl-1,2,3,6-tetrahydropyridine in mouse brain. J. Neurochem. 57, 348-351 https://doi.org/10.1111/j.1471-4159.1991.tb02134.x
  9. Chiba, K., E. T. Kubota, Y. Miyakawa, Y. Kato and T. B. Ishizaki. 1988. Characterization of hepatic microsomal metabolism as in vivo detoxication pathway of Nmethyl- 4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) in mice. J. Pharm. Exp. 246, 1108-1115
  10. Chiba, K., A. Trevor. and N. J. Castagnoli, 1984. Metabolism of the neurotoxic tertiary amine, MPTP, by brain monoamine oxidase. Biochem. Biophys. Res. Commun. 120, 574-578 https://doi.org/10.1016/0006-291X(84)91293-2
  11. Dombs, G. F. Jr. and S. B. Combs. 1984. The nutritional biochemistry of selenium. Ann. Rev. Nutr. 4, 254-280
  12. Gao R., Z. Yuan, Z. Zhao and X. Gao,.. 1998. Mechanism of pyrogallol autoxidation and determination of superoxide dismutase enzyme activity. Biol. Chem. 45, 41-45
  13. Harrison, I., D. L. John and G. S. Fell. 1996. DisAribution of selenium in human blood plasma and serum. Amalyct 121, 189-194
  14. Javoy-Agid, F. and Y. Agid. 1980. Is the mesocortical dopaminergic system involved in Parkinson's disease? Neurology 30, 1326-1330 https://doi.org/10.1212/WNL.30.12.1326
  15. Kojima, S., O. T. Matsuki, T. Nomura, M. T. Yamaoka and E. Niki, 1999. Elevation of antioxidant potency in the brain of mice by low dose ${\gamma}-ray$ irradiation and its effect on 1-methyl-4-phenyl-1,2,3,6-tetrahy-dropyridine (MPTP) induced brain damage. Free Radical Biology & Medicine 26, 388-395 https://doi.org/10.1016/S0891-5849(98)00200-7
  16. Kalaria, R. N., M. J. Mitchell and S. I. Harik. 1987. Correlation of 1-methyl-4-pheny-1,2,3,6-tetrahydropyridine neurotoxicity with blood brain barrier monoamine oxidase activity. Proc. Natl. Acad. Sci. USA. 84, 3521-3525
  17. Langstone, J. W., P. Ballard and R. A. Irwin. 1983. Chronic parkinsonism in humans due to a product of meperidine analog synthesis. Science 219, 979-980 https://doi.org/10.1126/science.6823561
  18. Lawrene, R. A. and R. F. Burk. 1976. Glutathione peroxidase activity in selenium deficiet rat liver. Biochem. Biophys. Res. Commun. 71, 952-958 https://doi.org/10.1016/0006-291X(76)90747-6
  19. Leibovitz, B. E. and B. V. Siegel. 1980. Aspects of free radical reactions in biological systems. Aging J. Gerontol. 35, 45-59 https://doi.org/10.1093/geronj/35.1.45
  20. Little, C. and P. J. O'Brine. 1968. An intracellular GSH -peroxidase with lipid peroxide substrate. Biochem. Biophys. Res. Commum. 31, 145-150 https://doi.org/10.1016/0006-291X(68)90721-3
  21. Lowry, O. H., N. J. Rodebrough, A. L. Farr and R. J.. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265-275
  22. Markey, S. P., J. N. Johannesse, C. S. Chiuen and M. A. Herkenham. 1984. Intraneuronal generation of a pyridinium metabolic may cause drug-induced parkinsonism. Nature 311, 464-467 https://doi.org/10.1038/311464a0
  23. Mize, C. E. and R. G. Langdon. 1962. Hepatic glutathione reductase purification and general kinetic properties. J. Biol. Chem. 237, 1589-1595
  24. Ohkawa, H., N. Ohishi and K. Yaki, 1979. Assay for lipid peroxide in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351-358 https://doi.org/10.1016/0003-2697(79)90738-3
  25. Paglia, E. D. and W. N. Valentin. 1967. Studies on the quantitative and qualitative charaterization of erythrocytes glutathione peroxidase. J. Lab. Clin. Med. 70, 158-69
  26. Reddy, C. C., C. P. D. Tu, J. R. Burgess, C. Y. Ho, R. W. Scholz and E. J. Massaro. 1981. Evidence for the occurrence of selenium - independent glutathione peroxidase activity in rat liver microsome. Biochem. Biophys. Res. Commun. 101, 970-978 https://doi.org/10.1016/0006-291X(81)91844-1
  27. Rhee, S. J. and W. K. Choi. 1991 Effect of heated oil and vitamin E on lipid peroxidative liver damage in rat. J. Korean Soc. Food Nutr. 20, 111-120
  28. Robert, F. L. and A. F. Clement. 1977. Effect of iron status on ${\delta}-aminolevulinic$ acid dehydratase activity. Biol. Med. 18, 323-329
  29. Sakurai, H. and K. A. Tsuchya. 1975. Tentative recommendation for the maximum daily intake of selenium. Environm. Phys. Biochem. 5, 107-118
  30. Sourkes, T. L. 1989, Disorders of basal ganglia. In Basic Neurochemistry. pp. 811, 4th Eds., Raven Press, New York
  31. Snyder, S. H. and R. J. D'Amato. 1986. MPTP. A neurotoxin relevant to the pathophysiology of Parkinson's disease. Neurology 36, 250-258 https://doi.org/10.1212/WNL.36.2.250
  32. Sriram, K., K. S. Pai, M. R. Boyd and V. Ravindranath. 1997. Evidence for generation of oxidative stress in brain by MPTP: in vitro and in vivo studies in mice. Brain Research 749, 44-52 https://doi.org/10.1016/S0006-8993(96)01271-1
  33. Suenatsu, T., T. Kamada, H. Abe, S. Kikuchi and K. Yagi. 1977. Serum lipoperoxide levels in patients suffering from liver disease. Clin. Chem. Acta 79, 267-270 https://doi.org/10.1016/0009-8981(77)90486-7
  34. Tanner, C. M. and J. W. Langston. 1990. Do environmental toxins cause Parkinson's disease? A critical review. Neurology 40, 17-30
  35. Thiffault, C., N. Aumont, R. Quirion and J. Poirier. 1995. Effect of MPTP and L-Deprenyl on antioxidants enzymes and lipid peroxidation level in mouse brain. J. Neuro. Int. Soc. Neurochemistry 2725-2733
  36. Welyer, W. and J. I. Salach. 1985. Purification and properties of mitochondrial monoamine oxidase type A from human placenta. J. Biological Chemistry 260, 13199-13207
  37. Wong, S. S., R. H. Y. Li and A. Stadlin. 1999. Oxidative stress induced by MPTP and $MPP^+$: selective nulherability of cultured astrocytes. Brain Research 836, 237-244 https://doi.org/10.1016/S0006-8993(99)01661-3
  38. Zou, L. L., J. Xu, Y. Jankovic, S. H. He, and W. D. Le. 2000. Pramipexole inhibititis lipid peroxidation and reduced injury in the substantia nigra induced by the dopaminergic neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetra- hydropyridine in C57BL/6 mice. Neuroscience Letters 281, 167-170 https://doi.org/10.1016/S0304-3940(00)00853-3

피인용 문헌

  1. Neuroprotective effects of Sohaphwangwon essential oil in a Parkinson's disease mouse model vol.23, pp.1, 2012, https://doi.org/10.7231/JON.2012.23.1.129