Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effect of Polymer Shielding on Elution of G3PDH Bound to Dye-ligand Adsorbent

  • Ling Tau Chuan (Biochemical Recovery Group, Centre for Formulation Engineering, Department of Chemical Engineering, University of Birmingham, Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia) ;
  • Lyddiatt Andrew (Biochemical Recovery Group, Centre for Formulation Engineering, Department of Chemical Engineering, University of Birmingham, Millipore Biopharmaceutical Division, Bioprocessing Ltd)
  • Published : 2006.01.01
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Abstract

Batch binding experiments were performed to assess the recovery performance of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) bound to the unshielded and polymer (polyvinyl pyrrolidone. PVP)-shielded dye-ligand (Cibacron Blue 3GA) adsorbent. The adoption of a polymer-shielded, dye-ligand technique facilitated the elution efficiency of bound G3PDH. It was demonstrated that the recovery of G3PDH using polymer-shielded dye-ligand adsorption yielded higher elution efficiency, at 60.5% and a specific activity of 42.3 IU/mg, after a low ionic strength elution (0.15 M NaCl). The unshielded dye-ligand yielded lower elution efficiency. at 6.5% and a specific activity of 10.2 IU/mg.

Keywords

References

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