Journal of Mechanical Science and Technology

The Korean Society of Mechanical Engineers (대한기계학회)
권호 표지

Contractile Force Measurements of Cardiac Myocytes Using a Micro-manipulation System

  • Park Suk-Ho (Microsystem Research Center, Korea Institute of Science and Technology) ;
  • Ryu Seok-Kyu (Microsystem Research Center, Korea Institute of Science and Technology) ;
  • Ryu Seok-Chang (Microsystem Research Center, Korea Institute of Science and Technology) ;
  • Kim Deok-Ho (Microsystem Research Center, Korea Institute of Science and Technology) ;
  • Kim Byung-Kyu (School of Aerospace & Mechanical Engineering, Hankuk Aviation University)
  • Published : 2006.05.01
Download PDF
⟨ Previous Next ⟩

Abstract

In order to develop a cell based robot, we present a micro-mechanical force measurement system for the biological muscle actuators, which utilize glucose as a power source. The proposed measurement system is composed of a micro-manipulator, a force transducer with a glass probe, a signal processor, an inverted microscope and video recording system. Using this measurement system, the contractile force and frequency of the cardiac myocytes were measured in real time and the magnitudes of the contractile force of each cardiac myocyte under different conditions were compared. From the quantitative experimental results, we could estimate that the force of cardiac myocytes is about $20\sim40{\mu}N$, and show that there are differences between the control cells and the micro-patterned cells.

Keywords

References

  1. Balaban, N. et al., 2001, 'Force and Focal Adhesion Assembly : a Close Relationship Studied Using Elastic Micro Patterned Substrates,' Nature Cell Biology, Vol. 3, pp. 466-472 https://doi.org/10.1038/35074532
  2. Jon, S., Seong, J., Khademhosseini, A., Tran, T. T., Laibinis, P. and Langer, R., 2003, 'Construction of Nonbiofouling Surfaces by Polymeric Self-Assembled Monolayers,' Langmuir, Vol. 19, pp. 9989-9993 https://doi.org/10.1021/la034839eS0743-7463(03)04839-X
  3. Jung, J., Kim, B., Tak, Y. and Park, J., 2003, 'Undulatory Tadpole Robot (TadRob) Using Ionic Polymer Metal Composite (IPMC) Actuator,' Int. Conf. Intelligent Robots and Systems, Vol. 3, pp. 2133-2138 https://doi.org/10.1109/IROS.2003.1249186
  4. Jungyul Park, et aI., 2005, 'Real-Time Measurement of the Contractile Forces of Self-Organized Cardiomyocytes on Hybrid Biopolymer Microcantilevers,' Analytical Chemistry, Vol. 10, ？No. 5, pp. 601-606
  5. Khademhosseini, A., Jon, S., Suh, K. Y., Tran, T. T., Eng, G., Yeh, J., Seong, J. and Langer, R., 2003, 'Direct Patterning of Protein- and Cell-Resistant Polymeric Monolayers and Microstructures,' Advanced Materials, Vol. 15, pp. 1995-2000 https://doi.org/10.1002/adma.200305433
  6. Lin, G., Palmer, E., Pister, K. and Roos, K., 2001, 'Miniature Heart Cell Force Transducer System Implemented in MEMS Technology,' IEEE Trans. Biomedical Engineering, Vol. 48, No. 9, pp. 996-1006 https://doi.org/10.1109/10.942589
  7. Phee, L., Accoto, D., Menciassi, A., Stefanini, C., Carrozza, M. C. and Dario, P., 2002, 'Analysis and Development of Locomotion Devices for the Gastrointestinal Tract,' IEEE Trans. Biomedical Engineering, Vol. 49, No. 6, pp. 613-616 https://doi.org/10.1109/TBME.2002.1001976
  8. Wojcikiewicz, E., Zhang, X. and Moy, V., 2004, 'Force and Compliance Measurements on Living Cells Using Atomic Force Microscopy (AFM),' Biological Procedures Online, Vol. 6, No. 1, pp. 1-9 https://doi.org/10.1251/bpo67
  9. Xi, J., Schmidt, J. and Montemagno, C., 2004, 'First Self-Assembled Micro-Robots Powered by Muscle,' SPIE Nanotechnology e-bulletin, 6
  10. Yin, S., Zhang, X., Zhan, C., Wu, J. and Cheung, J., 2004, 'Measuring Single Cardiac Myocyte Contractile Force Via Moving a Magnetic Bead,' Biophys J. BioFAST https://doi.org/10.1529/biophysj.104.048157
  11. Yu Sun and Bradley J. Nelson, 2004, 'MEMS ？for Cellular Force Measurements and Molecular Detection,' International Journal of Information Acquisition, Vol. 1, No. 1, pp. 23-32 https://doi.org/10.1142/S0219878904000136
  12. Zhao, Y., Yu, H. and Zhang, X., 2005, 'Creating Polymer-based Microstructures with Various Aspect Ratios from a Single Template for Cellular Force Measurements,' Proceeding of the 18th IEEE International Conference on Micro Electro Mechanical Systems (MEMS '05), Miami Beach, FL, USA, January 30-February 3, in press https://doi.org/10.1109/MEMSYS.2005.1453975
  13. http://www.foresight.org, Foresight Institute, USA
  14. http://www.nanotechnik.com
  15. http://www.sensorone.com
  16. http://www.transducertechniques.com
  17. http://www.vishay.com
  18. http://www.dspaceinc.com
  19. http://www.invitrogen.com
  20. http://www.headwayresearch.com
  21. http://www.sigmaaldrich.com
  22. http://www.zeiss.com
  23. http://www.nikon.com
  24. van Vilet, J., Bao, G. and Suresh, S., 2003, 'The Biomechanics Toolbox ; Experimental Approaches for Living Cells and Biomolecules,' Acta Materialia, 51, pp. 5881-5905 https://doi.org/10.1016/j.actamat.2003.09.001
  25. van der Velden, J. et al., 1998, 'Force Production in Mechanically Isolated Cardiac Myocytes from Human Ventricular Muscle Tissue,' Cardiovascular Research, 38, pp. 414-423 https://doi.org/10.1016/S0008-6363(98)00019-4