Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Monitoring of Aflatoxin $B_1$ in Livestock Feeds Using ELISA and HPLC

  • Han Eun-Mee (Division of Bioscience & Biotechnology, Konkuk University) ;
  • Park Hee-Ra (National Institute of Toxicological Research) ;
  • Hu Soo-Jung (National Institute of Toxicological Research) ;
  • Kwon Ki-Sung (National Institute of Toxicological Research) ;
  • Lee Hyo-Min (National Institute of Toxicological Research) ;
  • Ha Mi-Sun (Division of Bioscience & Biotechnology, Konkuk University) ;
  • Kim Kyung-Mi (Division of Bioscience & Biotechnology, Konkuk University) ;
  • Ko Eun-Jung (Division of Bioscience & Biotechnology, Konkuk University) ;
  • Ha Sang-Do (Department of Food Science and Technology, Chung-Ang University) ;
  • Chun Hyang-Sook (Korea Food Research Institute) ;
  • Chung Duck-Hwa (Department of Food Science & Technology, Gyeongsang National University) ;
  • Bae Dong-Ho (Division of Bioscience & Biotechnology, Konkuk University)
  • Published : 2006.04.01
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Abstract

Because of potential health hazards of aflatoxins for humans, the present study was conducted to monitor aflatoxin $B_1\;(AFB_1)$ in livestock feeds. A total of 249 samples of feeds collected in Korea were analyzed by DC-ELISA for qualitative analysis of $AFB_1$. Then, 27 samples that were verified to contain $AFB_1$ by DC-ELISA were quantitated by HPLC/FLD. HPLC/FLD analysis revealed that only one sample collected from a farm contained 11 ppb of $AFB_1$, whereas the other samples collected from feed companies did not contain $AFB_1$. The presence of $AFB_1$ was further confirmed by LC/MS analysis. TLC analysis indicated that the result of the DC-ELISA was most likely due to possible contamination of other mycotoxins rather than $AFB_1$. In conclusion, HPLC/FLD analysis following DC-ELISA is necessary for rapid and accurate detection of $AFB_1$.

Keywords

References

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