Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characteristics of Mediated Enzymatic Nitrate Reduction by Gallocyanine-Bound Nanoporous Electrode

  • Kim Seung-Hwan (Interdisciplinary Program for Biochemical Engineering and Biotechnology) ;
  • Song Seung-Hoon (Bio-MAX Institute) ;
  • Yoo Young-Je (Interdisciplinary Program for Biochemical Engineering and Biotechnology, School of Chemical Engineering, Seoul National University)
  • Published : 2006.04.01
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Abstract

A gallocyanine-bound nanoporous titanium dioxide electrode system was investigated to carry out a mediated enzyme reaction. Gallocyanine was bound either directly or through an aminopropylsilane linker to the film of nanoporous titanium dioxide and used as a mediator for nitrate reductase in the mediated enzymatic nitrate reduction. The electrode with the aminopropylsilane linker showed 20% higher efficiency of electron transfer at the same potential than that directly linked. The prepared electrodes showed $0.26{\mu}mol/h$ nitrate reduction at a $100mm^2$ surface of the electrode, and linear current response on nitrate ion concentration up to 1.0 mM, which is very useful as a biosensor of nitrate ion in water.

Keywords

References

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