Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Distinct Effects of Lysophospholipids on Membrane Potential in C6 Glioma Cells

  • Lee Yun-Kyung (Laboratory of Pharmacology, College of Pharmacy and Research Institute for Drug Development, Pusan National University) ;
  • Im Dong-Soon (Laboratory of Pharmacology, College of Pharmacy and Research Institute for Drug Development, Pusan National University)
  • Published : 2006.03.01
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Abstract

We tested effects of bioactive lysophospholipids including lysophosphatidic acid (LPA), lysophosphatidylcholine (LPC), sphingosylphosphorylcholine (SPC), and sphingosine I-phosphate (S1P) on membrane potential in C6 glioma cells to understand action mechanism of the lysophospholipids. Membrane potential was estimated by measuring fluorescence change of DiBAC-loaded glioma cells. LPA largely increased membrane potential and the increase was gradually diminished. LPC also increased the membrane potential, however, the increase sustained. SPC induced smaller increase of membrane potential than LPC. SIP was not able to change the membrane potential. We tested effects of suramin and pertussis toxin on lysophospholipid-induced membrane potential increase. However, there wasn't any effect. The membrane potential increase was partially diminished in $Na^+$-free media, suggesting $Na^+$ influx as a component of membrane potential changes. Thus, involvement of $Na^+$ influx in the increase of membrane potential by lysophospholipids and independence of suramin-sensitive GPCRs and pertussis toxin-sensitive G proteins are found in this study.

Keywords

References

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