Asian Journal of Turfgrass Science (아시안잔디학회지)

Turfgrass Society of Korea (한국잔디학회)
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Development of Antagonistic Microorganism for Biological Control of Dollar Spot of Turfgrass

잔디 동전마름병의 생물학적 방제를 위한 길항 미생물의 선발과 효력 검정

  • 신택수 ((주)그린바이오텍 부설 생명공학연구소) ;
  • 정우철 ((주)그린바이오텍 부설 생명공학연구소) ;
  • 도기석 ((주)그린바이오텍 부설 생명공학연구소) ;
  • 심규열 (한국잔디연구소) ;
  • 이재호 ((주)그린바이오텍 부설 생명공학연구소) ;
  • 최기현 ((주)그린바이오텍 부설 생명공학연구소)
  • Published : 2006.12.30
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Abstract

Dollar spot caused by Sclerotinia homeocarpa is one of major diseases in putting greens. Microorganisms antagonistic to S. homeocarpa, a pathogen of dollar spot, were primarily screened through in vitro tests, including dual culture method and triple layer agar diffusion method. In vivo tests were also conducted to select the best candidate for a biocontrol microorganism, using pot experiment. Bacillus subtilis EW42-1 and Trichoderma harziaum GBF-0208 were finally selected as biocontrol agents against dollar spot. Relative Performance Index(RPI) was used as a criterion of selecting potential biocontrol agents. B. subtilis EW42-1 and T. harzianum GBF-0208 showed resistance to several agrochemicals mainly used in a golf course. B. subtilis EW42-1 and T. harzianum GBF-0208 suppressed effectively the disease progress of dollar spot like synthetic fungicide tebuconazole in the nursery where dollar spot had seriously occurred. B. subtilis EW42-1 and T. harzianum GBF-0208 have a potential to be biocontrol agents for the control of dollar spot.

퍼팅 그린의 한지형 잔디에 많은 문제가 되고 있는 동전마름병은 Sclerotinia homeocarpa에 의해 발생한다. 본 연구에서는 미생물 제제를 이용한 생물학적 방제법을 통하여 동전마름병을 방제하고자 전국의 골프장과 산, 밭 토양에서 길항 미생물을 분리하였다. 길항 미생물은 동전마름병의 병원균인 S. homeocarpa에 대한 항균활성 및 균사 생장 억제력, 배양성 등을 고려하여 선발하였고, 폿트와 퍼팅 그린의 예비 묘포지에서의 생물 검정 실험을 통하여 효력을 검정하였다. 선발된 미생물은 Bacillus subtilis EW42-1과 Trichoderma harzianum GBF-0208이었고, 생물 검정 실험 결과 화학 농약 대조구인 터부코나졸 유제와 대비하여 높은 방제 효과를 보였다.

Keywords

References

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