Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production of an Antihyperlipemial HMG-CoA Reductase Inhibitor from Bacillus cereus D-3

Bacillus cereus D-3로부터 항고지혈증 HMG-CoA Reductase 저해제의 생산

  • Lee Dae-Hyoung (Department of Life Science and Genetic Engineering, Paichai University) ;
  • Lee Jae-Won (Department of Life Science and Genetic Engineering, Paichai University) ;
  • Jeong Jae-Hong (Agriculture Venture Team. Chungnam Agriculture Techno-Park) ;
  • Lee Jong-Soo (Department of Life Science and Genetic Engineering, Paichai University)
  • 이대형 (배재대학교 생명유전공학과) ;
  • 이재원 (배재대학교 생명유전공학과) ;
  • 정재홍 (충남농업테크노파크 벤처농업육성팀) ;
  • 이종수 (배재대학교 생명유전공학과)
  • Published : 2006.03.01
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Abstract

For the purpose of production of a novel antihyperlipemial HMG-CoA reductase inhibitor from bacteria, a bacterium which showed the highest HMG-CoA reductase inhibitory activity was isolated from traditional Doenjang. This strain was identified as Bacillus cereus (D-3) based on its microbiological characteristics and 165 rRNA sequence analysis. The maximal HMG-CoA reductase inhibitor production from Bacillus cereus D-3 was obtained by cultivation in a Glucose-CSL broth containing 2% glucose, 0.6% corn steep liquor, $0.04%\;K_{2}HPO_4$ and $0.05%\;KH_{2}PO_4$ at $30^{\circ}C$ for 36 h. The final HMG-CoA reductase inhibitory activity under the above conditions was 39.4%.

새로운 고지혈증 예방 제품 개발을 위한 기초 자료를 얻고자 우선 전통 된장으로부터 HMG-CoA reductase 저해 물질을 강력하게 세포외로 생산하는 D-3 세균을 최종 분리 하였다. D-3 균의 미생물학적 특성을 조사한 결과 그람양성의 간균으로 운동성이 있었고 통성 혐기성이였다. 이들 특성과 16S rRNA 염기서열의 분석 결과 등을 종합하여 Bergey's manual로 동정한 결과 Bacillus cereus D-3로 동정되었다. HMG-CoA reductase 저해물질 생산 최적 조건을 조사한 결과 Bacillus cereus D-3를 glucose 2%, corn steep liquor $0.6%,\;K_{2}HPO_4\;0.04%,\;KH_{2}PO_4\;0.05%$를 함유한 Glucose-CSL 배지에 접종하여 $30^{\circ}C$에서 36시간 배양하여 얻은 상등액의 HMC-CoA reductase 저해활성이 39.4% 저해물질을 가장 많이 생성 하였다.

Keywords

References

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