Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Classification of Listeria monocytogenes Isolates from Korean Domestic Foods Using Random Amplification of Polymorphic DNA and Serotyping Analysis

Random Amplification of Polymorphic DNA와 혈청학적 분석을 이용한 국내식품에서 분리한 Listeria monocytogenes의 분류

  • Kim Hyun-Joong (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Park Si-Hong (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Kim Hae-Yeong (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University)
  • 김현중 (경희대학교 생명자원과학연구소 및 생명과학대학 식품생명공학과) ;
  • 박시홍 (경희대학교 생명자원과학연구소 및 생명과학대학 식품생명공학과) ;
  • 김해영 (경희대학교 생명자원과학연구소 및 생명과학대학 식품생명공학과)
  • Published : 2006.03.01
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Abstract

Molecular subtyping of Listeria monocytogenes, including type strains and isolates from Korean foods, were performed using random amplification of polymorphic DNA (RAPD). Each Listeria species showed specific RAPD band patterns, and L. monocytogenes serotypes and isolates were divided into two clusters. RAPD results showed that L. monocytogenes isolates from Korean foods were divided into two groups. Group I contained L. monocytogenes serotypes 1/2b and 4b, whereas Group II contained serotypes 1/2a and 1/2c. These results suggested RAPD as possible subtyping methods for Listeria species. Also, RAPD Results showed significant correlation between molecular subtyping and serotyping of L. monocytogenes, and classified two different groups of L. monocytogenes isolated from Korean foods.

본 연구에서는 국내시장에서 유통되는 육류, 냉동식품, 생우유, 조개류 등과 같은 식품으로부터 L. monocytogenes들을 분리하고 혈청형을 결정하였다. RAPD 결과를 통해서 L. monocytogenes는 Listeria 속의 다른 균주들과 전체 밴드의 수와 크기에서 구별이 되었다. 또한, L. monocytogenes는 2개 그룹으로 구별되었으며, 그룹 I은 L. monocytogenes 1/2b, 4e, 4b, 그룹 II는 L. monocytogenes 1/2a, 1/2c, 3 혈청형 그룹별로 분류되었다. 결론적으로 RAPD 방법과 serotyping은 L. monocytogenes를 분류하는 데에 있어서 기존의 방법의 단점을 보완한 새로운 가능성을 제시하였다.

Keywords

References

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