Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enzymatic Activities in Petroleum Wastewater Purification System by an Activated Sludge Process

  • Li Yin (College of Environmental Science & Engineering, Dong Hua University) ;
  • Chrost Ryszard J. (Department of Microbial Ecology, Institute of Microbiology, Warsaw University)
  • Published : 2006.02.01
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Abstract

The enzymology of an activated sludge system for a petroleum wastewater purification process was investigated. Leucine-aminopeptidase (L-AMP), ${\beta}$-glucosidase (${\beta}-GLC$), and lipase (LIP) were selected for the study. It was found that more than 81.7% of enzymatic activity was associated with microbial cells in the activated sludge floc. The metabolic response of a mixed microbial population to increased phenol concentration showed that L-AMP activity increased in the activated sludge, whereas activities of ${\beta}-GLC$ and LIP decreased, due to the inhibitory effect of the phenol which varied from 100 mg/l to 500 mg/l.

Keywords

References

  1. APHA, 1998. Standard Methods for Examination of Water and Wastewater, 20th Ed. American Public Health Association, Washington, D.C., U.S.A
  2. Avci, A., M. Kacmaz, and I. Durak. 2005. Peroxidation in muscle and liver tissues from fish in a contaminated river due to a petroleum refinery industry. Ecotox. Environ. Safe. 60: 101-105 https://doi.org/10.1016/j.ecoenv.2003.10.003
  3. Barjenbruch, M. and O. Kopplow. 2003. Enzymatic, mechanical and thermal pre-treatment of surplus sludge. Adv. Environ. Res. 7: 715-720 https://doi.org/10.1016/S1093-0191(02)00032-1
  4. Boszczyk-Maleszak, H., M. Chorazy, E. Bieszkiewicz, and J. Kacieszczenko. 2002. Phenol utilisation by fungi isolated from activated sludge. Acta Microb. Pol. 51: 183-191
  5. Cadoret, A., A. Conrad, and J.-C. Block. 2002. Availability of low and high molecular weight substrates to extracellular enzymes in whole and dispersed activated sludges. Enzyme Microb. Tech. 31: 179-186 https://doi.org/10.1016/S0141-0229(02)00097-2
  6. Czerska, B., K. Miksch, N. Matsche, and A. Franz. 1997. Application of enzymatic activity measurements to biological phosphate removal from wastewater. Water Sci. Tech. 36: 87-95
  7. Gessesse, A., T. Dueholm, S. B. Petersen, and P. H. Nielsen. 2003. Lipase and protease extraction from activated sludge. Water Res. 37: 3652-3657 https://doi.org/10.1016/S0043-1354(03)00241-0
  8. Gianfreda, L., F. Sannino, M. A. Rao, and J.-M. Bollag. 2003. Oxidative transformation of phenols in aqueous mixtures. Water Res. 37: 3205-3215 https://doi.org/10.1016/S0043-1354(03)00154-4
  9. Guellil, A., F. Thomas, J.-C. Block. J.-L. Bersillon, and P. Ginertet. 2001. Transfer of organic matter between wastewater and activated sludge flocs. Water Res. 35: 143- 150 https://doi.org/10.1016/S0043-1354(00)00240-2
  10. Kibret, M., W. Somitsch, and K.-H. Robra. 2000. Characterization of a phenol degrading mixed population by enzyme assay. Water Res. 34: 1127-1134 https://doi.org/10.1016/S0043-1354(99)00248-1
  11. Lopez-Molina, D., A. N. P. Hiner, J. Tudela, F. Garcia- Canovas, and J. N. Rodriguez-Lopez. 2003. Enzymatic removal of phenols from aqueous solution by artichoke (Cynara scolymus L.) extracts. Enzyme Microb. Tech. 33: 738-742 https://doi.org/10.1016/S0141-0229(03)00208-4
  12. Nguyen, V. T. and W. K. Shieh. 1995. Anoxic and oxic biological fluidized bed treatment of amines and phenol. Water Sci. Tech. 31: 185-193
  13. Ou, S., J. Zheng, J. Zheng, B. J. Richardson, and P. K. S. Lam. 2004. Petroleum hydrocarbons and polycyclic aromatic hydrocarbons in the surficial sediments of Xiamen Harbour and Yuan Dan Lake, China. Chemosphere 56: 107-112 https://doi.org/10.1016/j.chemosphere.2004.02.022
  14. Raunkjaer, K., T. Hvitved-Jacobsen, and P. H. Nielsen. 1994. Measurement of pools of protein, carbohydrate and lipid in domestic wastewater. Water Res. 28: 251-262 https://doi.org/10.1016/0043-1354(94)90261-5
  15. Seetharam, G. B. and B. A. Saville. 2003. Degradation of phenol using tyrosinase immobilized on siliceous supports. Water Res. 37: 436-440 https://doi.org/10.1016/S0043-1354(02)00290-7
  16. Whiteley, C. G., J. E. Burgess, X. Melamane, B. Pletschke, and P. D. Rose. 2003. The enzymology of sludge solubilisation utilising sulphate-reducing systems: The properties of lipases. Water Res. 37: 289-296 https://doi.org/10.1016/S0043-1354(02)00281-6
  17. Wingender, J., K.-E. Jaeger, and H.-C. Flemming. 1999. Interaction between extracellular polysaccharides and enzymes, pp. 231-251. In Wingender, J., T. R. Neu, and H.-C. Flemming (eds.). Microbial Extracellular Substrances: Characterization, Substructure and Function. Springer, New York, U.S.A