동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제20권1호
  • /
  • Pages.20-24
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  • 2006
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

혈관내피세포에서 cisplatin에 의한 세포고사에 대한 산약보정방암탕 에탄을 추출물의 방어효과

Protective Effects of Sanyakbojungbangam-tang Ethanol Extracts on Cisplatin-induced Apoptosis in ECV304 Cells

  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 김은경 (원광대학교 한의과대학 생리학교실) ;
  • 이영래 (전북대학교 의과대학 생화학교실) ;
  • 주성민 (원광대학교 한의과대학 병리학교실) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실) ;
  • 김성훈 (경희대학교 한의과대학 병리학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실)
  • Kwon Kang-Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Eun-Kyung (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Lee Young-Rae (Department of Biochemistry, Chonbuk National University Medical School.) ;
  • Ju Sung-Min (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Ryu Do-Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Sung-Hoon (Department of Pathology, College of Oriental Medicine, KyungHee University) ;
  • Jeon Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University)
  • 발행 : 2006.02.01
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초록

This study was designed to investigate the protective effect of Sanyakbojungbangam-tang Ethanol Extracts (SB Et-OH) on the cisplatin-induced apoptosis of human endothelial cell line ECV304 cells. After cells were treated with cisplatin, MTT assay was performed for cell viability test. To explore the mechanism of cytotoxicity, we used the several measures of apoptosis to determine whether this processes was involved in cisplatin-induced cell damage in ECV304 cells. Also, cells were treated with SB Et-OH and then, followed by the addition of cisplatin. Cisplatin decreased the viability of ECV304 cells in a dose-dependent manner and increased the caspase-3 enzyme activity ECV304 cells treated cisplatin were revealed as apoptosis characterized by nuclear staining. SB Et-OH protected ECV304 cells from cisplatin-induced nuclear fragmentation and chromatin condensation. Also, SB Et-OH inhibited the activation of caspase-3 pretense and the cleavage of poly(ADP-ribose) polymerase (PARP) in cisplatin-treated ECV304 cells. According to above results, SB Et-OH may protect ECV304 cells from the apoptosis induced by cisplatin.

키워드

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