오동나무 잎 추출물의 항염 효능에 관한 연구

Studies on the Anti-inflammatory Activity of Paulownia coreana Uyeki Leaf Extract

  • Kim, Nam-Kyoung (Radiant Inc., Bioindustry Innovation Center, Hi-Tech Venture Town) ;
  • Kim, Mi-Hwa (Radiant Inc., Bioindustry Innovation Center, Hi-Tech Venture Town) ;
  • Yoon, Chang-Soon (Radiant Inc., Bioindustry Innovation Center, Hi-Tech Venture Town) ;
  • Choi, Shin-Wook (Radiant Inc., Bioindustry Innovation Center, Hi-Tech Venture Town)
  • 발행 : 2006.12.30

초록

본 연구는 피부에 안전하고 부작용이 없는 식물성 원료를 이용한 항염 소재를 개발하여 피부 진정 및 여드름, 아토피 피부에 적용되어질 수 있는 피부 자극 완화제를 개발하고자 하였다. 이에 따라 전통적으로 염증 질환 치료에 사용되어진 오동나무(Paulownia coreana Uyeki)를 소재로 하여 오동나무 잎 추출물의 항산화 효능과 항염 효능을 알아봄으로써 화장품 원료로의 가능성을 확인하였다. EPR을 이용하여 오동나무 잎 추출물의 superoxide anion radical 소거능을 확인하였으며 대식세포주에서의 LPS 처리에 의해 유도되어지는 NO의 생성을 저해시키는 효능을 확인하였다. 그 결과 추출물의 우수한 NO 생성 저해능을 확인하여 오동나무 잎 추출물의 항염 효능 메카니즘을 규명하였다. ELISA 기법을 이용하여 $PGE_2$ 생성 억제 효능을 확인하였으며 NO와 $PGE_2$ 생성 단백질인 iNOS와 COX-2의 발현 여부를 확인한 결과 오동나무 잎 추출물 처리에 의해 두 단백질의 발현이 확연히 감소되는 것을 확인하였다. 또한 염증 반응의 초기 단계에 관여하는 $IkB-{\alpha}$ 단백질의 분해정도를 시간별로 확인한 결과 LPS 처리 30 min 후 $IkB-{\alpha}$ 단백질의 분해가 일어나게 되는데 오동나무 잎 추출물을 함께 처리하였을 경우 시간이 경과되어도 $IkB-{\alpha}$ 단백질의 분해가 일어나지 않는 것을 확인하였다. 이로써 오동나무 잎 추출물의 항염 효능은 염증 반응 신호 전달의 초기 단계에 해당하는 $IkB-{\alpha}$ 단백질의 분해를 억제함으로써 결과적으로 NO와 $PGE_2$의 생성을 감소시킴을 확인하였다. 또한 CCD-986sk와 Raw 264.7 세포에 대한 세포 독성이 존재하지 않음을 확인함으로써 오동나무 잎의 항염 효능을 이용한 피부 자극완화제로서의 개발 가능성을 확인하였다.

This work was carried out to investigate the anti-inflammatory effects of Paulownia coreana Uyeki on abirritant, atopy and acne skin. Paulownia coreana Uyeki has been used as a traditional medicine having anti-febrile, anti-inflammation effect in Korea, Paulownia coreana Uyeki loaves were extracted with 70% EtOH. Its superoxide anion radical scavenging activity and inhibitory effect on LPS-induced NO production were examined. The extract inhibitied the generation of NO and $PGE_2$ induced by LPS in the macrophage cell line (Raw 264.7). Consistent with the inhibitory effects on No and $PGE_2$ generation, the extract inhibited expression of iNOS and COX-2. In further study, it was found that the extract prevented $IkB-{\alpha}$ degradation, as demonstrated by western blot analysis of $IkB-{\alpha}$ protein level. However, the extract treatment did not affect cell viability at $100{\mu}g/mL$ concentration in both human skin fibroblast and Raw 264.7 cells in vitro. Thus, the present study suggests that Paulownia coreana Uyeki leaves extract have significant anti-inflammatory activity and potential as an anti-irritation material.

키워드

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