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High Pressure Freezing (HPF)을 이용한 조류 Ptilota filicina의 미세구조 관찰:HPF 고정법과 화학 고정법의 비교

Ultrastructures of Ptilota filicina (Rhodophyta) by High Pressure Freezing(HPF): Comparison of HPF Fixation and Chemical Fixation

  • 이상희 (한국기초과학지원연구원 전자현미경연구부) ;
  • 김윤중 (한국기초과학지원연구원 전자현미경연구부) ;
  • 정종만 (한국기초과학지원연구원 전자현미경연구부) ;
  • 김진규 (한국기초과학지원연구원 전자현미경연구부) ;
  • 김영민 (한국기초과학지원연구원 전자현미경연구부) ;
  • 권희석 (한국기초과학지원연구원 전자현미경연구부) ;
  • 문원진 (한국기초과학지원연구원 광주센터) ;
  • 이석훈 (한국기초과학지원연구원 전자현미경연구부)
  • Lee, Sang-Hee (Division of Electron Microscopic Research, Korea Basic Science Institute) ;
  • Kim, Youn-Joong (Division of Electron Microscopic Research, Korea Basic Science Institute) ;
  • Jeong, Jong-Man (Division of Electron Microscopic Research, Korea Basic Science Institute) ;
  • Kim, Jin-Gyu (Division of Electron Microscopic Research, Korea Basic Science Institute) ;
  • Kim, Young-Min (Division of Electron Microscopic Research, Korea Basic Science Institute) ;
  • Kweon, Hee-Seok (Division of Electron Microscopic Research, Korea Basic Science Institute) ;
  • Moon, Won-Jin (KBSI, Gwang-ju Center) ;
  • Lee, Seok-Hoon (Division of Electron Microscopic Research, Korea Basic Science Institute)
  • 발행 : 2006.12.31

초록

In preparation of the biological samples for electron microscopy, the chemical fixation by glutaraldehyde, paraformaldehyde, and OsO4 has been generally used for a long time. However, the chemical fixation method has some problems: the infiltration time is a little bit long and the ultrastructure of cell or tissue transforms before complete fixation of sample. So, recently, cryo-fixation is considered more often in biomedical field. In this study, we compared High Pressure Freezing (HPF) method with chemical fixation method using a algal sample (Ptilota filicina J. Agardh), which was difficult to fix using chemical fixation method. In chloroplast, the ultrastructure of thylakoid lamella and phycobilisome can not show clearly by chemical fixation. In this study we could observe the ultrastructure of thylakoid lamella and phycobilisome of chloroplast very clearly using HPF fixation. An improved images of ultrastructures of nucleus, mitochondrion and floridean starch could obtain. These results suggest that HPF method is very useful method in algal specimen for electron microscopy.

키워드

참고문헌

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피인용 문헌

  1. Ascophyllumand its symbionts. X. Ultrastructure of the interaction betweenA. nodosum(Phaeophyceae) andMycophycias ascophylli(Ascomycetes) vol.86, pp.2, 2008, https://doi.org/10.1139/B07-122