Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
권호 표지
DOI QR코드

DOI QR Code

Inhibition Activity Against Pathogenic Organism of Probiotic Bacteria and Characterization of Inhibition Activity of Isolated Bacteria from Calf Dejecta

Probiotic균주의 Pathogenic Organism에 대한 억제 활성과 송아지분변 분리균주의 억제활성 특성

  • Published : 2006.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate the inhibitory activity of Lactobacillus spp., Bacillus ssp., and calf fecal isolates against pathogenic Salmonella typhimurium, E. coli, Listeria monocytogenes, and Staphylococcus aureus. Among thirteen strains of Lactobacillus ssp. tested, Lactobacillus helveticus CU631 showed the highest inhibition against three pathogens, whereas Bacillus spp. showed a weak inhibitory activity. Four calf fecal isolates were identified as Lactobacillus pentosus CU13, CU05, Pediococcus pentosaceus CUR02, and Lactobacillus lactis ssp. lactis CUM14. The whole cell and cell wall components of L. rhamnosus CU02 and L. pentosus CU13 were active in the inhibition of L. monocytogenes. The medium components and levels, which affect on the inhibitory activity, were revealed as Tween 80 1.0%, peptone 3.0%, yeast extract 3.0%, glucose 3.0%, beef extract 3.0%, and NaCl 1.0～3.0%, respectively. Inhibitory activity of the supernatant culture medium was not affected by catalase and proteinase K treatment but affected by heat treatment at 80℃ and netralization, which implies that the inhibitory activity is due to the production of organic acids during the growth. L. pentosus CU13 and L. rhamnosus CU02 exhibited broad inhibition spectrum against 16 out of 21 strains including some pathogens. Oral administration of L. rhamnosus CU02 to the mice infected with E. coli O157:H7 was proven to be effective to recover their body weight during the experimental period.

본 실험은 Lactobacillus spp.와 Bacillus spp. 의 Salmonella typhimurium, E. coli 및 Listeria monocytogenes에 대한 억제활성과 송아지 분변 분리균주의 생장 및 억제활성의 특성을 알아보기 위해 실시되었다.Lactobacillus spp.와 Bacillus spp.의 병원성 세균 Salmonella typhimurium에 대한 억제활성은 Lactobacillus helveticus CU631이 가장 높았고, Bacillus spp.는 활성이 약하였다. 송아지 분변 분리균주를 동정한 결과 Lactobacillus pentosus CU13과 CU05, Pediococcus pentosaceus CUR02, Lactococcus lactis ssp lactis CUM14로 확인되었다. Lactobacillus rhamnosus CU02와 Lactobacillus pentosus CU13의 Listeria monocytogenes에 대한 억제활성에 영향을 미치는 배지성분과 첨가수준은 Tween 80 1.0%, peptone 3.0%, yeast extract 3.0%, glucose 3.0% beef extract 3.0%, NaCl 1.0～3.0%이며, whole cell과 세포벽 물질은 Listeria monocytogenes에 대하여 억제활성을 나타내었다. 억제 성향을 강하게 보인 균체 배양액을 80℃로 열처리한 결과 억제력은 나타나지 않았으나 catalase 및 Proteinase-K 처리는 억제활성에 영향을 미치지 않았다. 이 결과 억제활성 물질은 유기산에 의한 것으로 사료된다. Lactobacillus pentosus CU13과 Lactobacillus rhamnosus CU02의 21균주에 대한 억제 능력을 측정한 결과 병원성균주를 포함한 16균주에서 억제활성을 보였으나 5균주에서는 억제성향을 나타내지 않았다. Escherichia coli O157:H7을 감염시킨 mouse 중 Lactobacillus pentosus CU13을 접종한 경우 다소 체중 회복 현상이 나타났으나, Lactobacillus rhamnosus CU02을 접종한 경우 체중 회복이 빠르게 나타났다.

Keywords

References

  1. Axelsson, L. and Lindgren, S. 1987. Characterization and DNA homology of Lactobacillus strains isolated from pig interstine. J. Appl. Bacteriol. 62:433-440 https://doi.org/10.1111/j.1365-2672.1987.tb02673.x
  2. Barrow, P. A., Fuller, R. and Newport, M. J. 1977. Changes in the microflora and physiology of the anterior tract of pigs weaned at 2 days, with special reference to the pathogenesis of diarrhea. Infect. Immun. 18:586-595
  3. Biswas, S. R., Ray, P., Johnson, M. C. and Ray, B. 1991. Influence of growth conditions on the production of a bacteriocin, pediocin ACH, by Pediococcus acidilactic H. Appl. Environ. Microbiol. 57:1265-1267
  4. Fuller, R. 1992. Probiotics: The scientific basis. champman and Hall. p.1-3
  5. Joerger, M. C. and Klaenhammer, T. R. 1986. Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481. J. Bacteriol. 167:439-446 https://doi.org/10.1128/jb.167.2.439-446.1986
  6. Jonsson, E. and Conway, P. 1992. Probiotics for pigs. In Probiotics- the science basis, ed R. Fuller, Champman and Hall, 260-316
  7. Ogunbanwo, S. T., Sanni, A. I. and Onilude, A. A. 2003. Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis. Afri. J. of Biotechnol. 2(8):219-227 https://doi.org/10.5897/AJB2003.000-1045
  8. Ogunbanwo, S. T., Sanni, A. I. and Onilude, A. A. 2003. Influence of cultural conditions on the production of bacteriocin by Lactobacillus brevis OG1. Afri. J. Biotechnol. 2(7):179-184 https://doi.org/10.5897/AJB2003.000-1037
  9. Piard, J. C., Delorme, F., Giraffa, G., Commissaire, J. and Desmazeaud, M. 1990. Evidence for a bacteriocin produced by Lactococcus lactis CNRZ 481. J. Neth. Milk Dairy. 44:143-158
  10. Raibaud, P., Caulet, M. and Galpin, J. V. 1961. Studies on the bacterial flora of the alimentary tract of pigs. II. Streptococci: selective enumer- ation and differentiation of the dominant group. J. Appl. Bacteriol. 24:285-306 https://doi.org/10.1111/j.1365-2672.1961.tb00262.x
  11. Robert, I. L., Michael, R., Sylvia, S. S., Harwig, L., Richard, J. and Patricia, E. 1991. Ultrasensitive assay for endogenous antimicrobial polypeptides. J. Immunologycal Method, 137:167-173 https://doi.org/10.1016/0022-1759(91)90021-7
  12. Robinson, I. M., Stromley, H. M. and Varel, V. H. 1988. Streptococcus intestinalis, a new species from the colons and feces of pigs. Int. J. Syst. Bacteriol. 38:245-248 https://doi.org/10.1099/00207713-38-3-245
  13. Robinson, I. M., Whipp, S. C. and Bucklin, J. A. 1984. Characterization of predominant bacteria from the colons of normal and dysenteric pigs. Appl. Environ. Microbiol. 24:285-306
  14. Schillinger, U. and Lucke, F. K. 1989. Antimicrobial activity of Lactobacillus sake isolated from meat. Appl. Environ. Microbiol. 55:1901-1906
  15. Steel, R. G. D. and Torrie, J. H. 1981. Principles and procedures of statistics, 2nd ed. McGraw-Hill, New York
  16. Venkitanarayanan, K. S., Lin, C.-M., Bailey, J. and Doyle, M. P. 2002. Inactivation of Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes on apples, oranges, and tomatoes by lactic acid with hydrogen peroxide. J. Food Protection. 65(1):100-105 https://doi.org/10.4315/0362-028X-65.1.100
  17. Zani, G., Biavati, B. and Crociani, F. 1974. Bifidobacteria from the feces of piglets. J. Appl. Bacteriol. 3:537-547