Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
권호 표지

Usefulness of the recombinant liver stage antigen-3 for an early serodiagnosis of Plasmodium falciparum infection

  • LEE Hyeong-Woo (Division of Tropical and Endemic Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention) ;
  • MOON Sung-Ung (Division of Tropical and Endemic Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention) ;
  • RYU Hye-Sun (Division of Tropical and Endemic Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention) ;
  • KIM Yeon-Joo (Division of Tropical and Endemic Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention) ;
  • CHO Shin-Hyeong (Division of Tropical and Endemic Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention) ;
  • CHUNG Gyung-Tae (Division of Tropical and Endemic Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention) ;
  • LIN Khin (Parasitology Division, Department of Medical Research (Upper Myanmar)) ;
  • NA Byoung-Kuk (Department of Molecular Parasitology, Sungkyunkwan University School of Medicine) ;
  • KONG Yoon (Department of Molecular Parasitology, Sungkyunkwan University School of Medicine) ;
  • CHUNG Kyung-Suk (Department of Clinical Pathology, Ansan College) ;
  • KIM Tong-Soo (Division of Tropical and Endemic Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention)
  • Published : 2006.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

In order to develop tools for an early serodiagnosis of Plasmodium falciparum infection, we evaluated the usefulness of P. falciparum liver stage antigen-3 (LSA-3) as a serodiagnostic antigen. A portion of LSA-3 gene was cloned, and its recombinant protein (rLSA-3) was expressed in Escherichia coli and purified by column chromatography. The purified rLSA-3 and 120 test blood/serum samples collected from inhabitants in malaria-endemic areas of Mandalay, Myanmar were used for this study. In microscopic examinations of blood samples, P. falciparum positive rate was $39.1\%$ (47/120) in thin smear trials, and $33.3\%$ (40/120) in thick smear trials. Although the positive rate associated with the rLSA-3 $(30.8\%)$ was lower than that of the blood stage antigens $(70.8\%)$, rLSA-3 based enzyme-linked immunosorbent assay could detect 12 seropositive cases $(10.0\%),$ in which blood stage antigens were not detected. These results indicate that the LSA-3 is a useful antigen for an early serodiagnosis of P. falciparum infection.

Keywords

References

  1. Aidoo M, Lalvani A, Gilbert SC, Hu JT, Daubersies P, Hurt N, Whittle HC, Druihle P, Hill A V (2000) Cytotoxic Tlymphocyte epitopes for HLA-B-53 and other HLA types in the malaria vaccine candidate liver stage antigen 3. Infect Immun 68: 227-232 https://doi.org/10.1128/IAI.68.1.227-232.2000
  2. Baird JK (2004) Chloroquine resistance in Plasmodium vivax. Antimicrob Agents Chemother 48: 4075-4083 https://doi.org/10.1128/AAC.48.11.4075-4083.2004
  3. Breman JG (2001) The ears of the hippopotamus: manifestations, determinants, and estimates of the malaria burden. Am J Trop Med Hyg 64: 1-11
  4. Chai IH, Lim GI, Yoon SN, Oh WI, Kim SJ, Chai JY (1994) Occurrence of tertian malaria in a male patient who has never been abroad. Korean J Parasitol 32: 195-200 https://doi.org/10.3347/kjp.1994.32.3.195
  5. Cho SY, Kong Y, Park SM, Lee JS, Lim YA, Chae SL, Kho WG, Lee JS, Shim JC, Shin HK (1994) Two vivax malaria cases detected in Korea. Korean J Parasitol 32: 281-284 https://doi.org/10.3347/kjp.1994.32.4.281
  6. Daubersies P, Thomas AW, Millet P, Brahimi K, Langermans JA, Ollomo B, BenMohamed L, Slierendregt B, Eling W, Van Belkum A, Dubreuil G, Meis JF, Guerin-Marchand C, Cayphas S, Cohen J, GrasMasse H, Druilhe P (2000) Protection against Plasmodium falciparum malaria in chimpanzees by immunization with the conserved pre-erythrocytic liverstage antigen 3. Nature Med 6: 1258-1263 https://doi.org/10.1038/81366
  7. Gregson A, Plowe CV (2005) Mechanisms of resistance of malaria parasites to antifolates. Pharmacol Rev 57: 117-145 https://doi.org/10.1124/pr.57.1.4
  8. Guerin-Marchand C, Druilhe P, Galey B, Londono A, Patarapotikul J, Beaudoin RL, Dubeaux C, Tartar A, Mercereau-Puijalon O, LangsleyG (1987) A liver-stagespecific antigen of Plasmodium falciparum characterized by gene cloning. Nature 329: 164-167 https://doi.org/10.1038/329164a0
  9. Joshi SK, Bharadwaj A, Chatterjee S, Chauhan VS (2000) Analysis of immune response against T-and B-cell epitopes from Plasmodium falciparum liver stage antigen 1 in rodent malaria models and malaria exposed human subject in India. Infect Immun 68: 141-150 https://doi.org/10.1128/IAI.68.1.141-150.2000
  10. Kurtis JD, Hollingdale MR, Luty AJ, Lanar DE, Krzych U, Duffy PE (2001) Pre-erythrocytic immunity to Plasmodium falciparum: the case for an LSA-1 vaccine. Trends Parasitol 17: 219-223 https://doi.org/10.1016/S0169-4758(00)01862-7
  11. Perlaza BL, Sauzet JP, Balde AT, Brahimi K, Tall A, Corradin G, Druilhe P (2001) Long synthetic peptides encompassing the Plasmodium falciparum LSA3 are the target of human B and T cells and are potent inducers of B helper, T helper and cytolytic T cell responses in mice. Eur J Immunol 31: 2200-2209 https://doi.org/10.1002/1521-4141(200107)31:7<2200::AID-IMMU2200>3.0.CO;2-L
  12. Roper C, Pearce R, Bredenkamp B, Gumede J, Drakeley C, Mosha F, Chandramohan D, Sharp B (2003) Antifolate antimalarial resistance in southeast Africa: a population-based analysis. Lancet 361: 1174-1181 https://doi.org/10.1016/S0140-6736(03)12951-0
  13. Sauzet JP, Perlaza BL, Brahimi K, Daubersies P, Druilhe P. (2001) DNA immunization by Plasmodium falciparum liver-stage antigen 3 induces protection against Plasmodium yoelii sporozoite challenge. Infect Immun 69: 1202-1206 https://doi.org/10.1128/IAI.69.2.1202-1206.2001
  14. Snow RW, Trape JF, Marsh K (2001) The past, present and future of childhood malaria mortality in Africa. Trends Parasitol 17: 593-597 https://doi.org/10.1016/S1471-4922(01)02031-1
  15. Taylor-Robinson AW (2003) Immunity to liver stage malaria: considerations for vaccine design. Immunol Res 27: 53-70 https://doi.org/10.1385/IR:27:1:53
  16. Wellems TE, Plowe CV (2001) Chloroquine-resistant malaria. J Infect Dis 184: 770-776 https://doi.org/10.1086/322858
  17. Yeom JS, Ryu SH, Oh S, Lee WJ, Kim TS, Kim KH, Kim YA, Ahn SY, Cha JE, Park JW (2005) Status of Plasmodium vivax malaria in the Republic of Korea during 2001-2003. Am J Trap Med Hyg 73: 604-608