Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
권호 표지

Identification of novel Leishmania major antigens that elicit IgG2a response in resistant and susceptible mice

  • MOHAMMADI Mohammad Reza (Institute of Biochemistry and Biophysics, University of Tehran) ;
  • ZEINALI Majid (Institute of Biochemistry and Biophysics, University of Tehran) ;
  • ARDESTANI Sussan K. (Institute of Biochemistry and Biophysics, University of Tehran) ;
  • KARIMINIA Amina (Department of Immunology, Pasteur Institute of Iran)
  • Published : 2006.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Experimental murine models with high, intermediate and low levels of genetically based susceptibility to Leishmania major infection reproduce almost entire spectrum of clinical manifestations of the human disease. There are increasing non-comparative studies on immune responses against isolated antigens of L. major in different murine strains. The aim of the present study was to find out whether there is an antigen that can induce protective immune response in resistant and susceptible murine strains. To do that, crude antigenic extract of procyclic and metacyclic promastigotes of L. major was prepared and subjected to SDS-PAGE electrophoresis. Western-blotting was used to search for antigen(s) capable of raising high antibody level of IgG2a versus IgG1 in the sera of both infected resistant and susceptible strains. Two novel antigens from metacyclic promastigotes of L. major (140 and 152 kDa) were potentially able to induce specific dominant IgG2a responses in BALB/c and C57BU6 mice. The 2 antigens also reacted with IgG antibody of cutaneous leishmaniasis patients. We confirm that 140 and 152 kDa proteins of L. major promastigotes are inducing IgG production in mice and humans.

Keywords

References

  1. Afrin F, Anam K, Ali N (2000) Induction of partial protection against Leishmania donovani by promastigote antigens in negatively charged liposomes. J Parasitol 86: 730-735 https://doi.org/10.1645/0022-3395(2000)086[0730:IOPPAL]2.0.CO;2
  2. Aragort De Rossell R, Calcagno M, Rossell 0, Maizo De Segnini Z, Rodriguez AM (2001) Intralesional isotype profiles in human localized cutaneous leishmaniasis lesions. Int J Exp Pathol 82: 129-134 https://doi.org/10.1111/j.1365-2613.2001.iep184.x
  3. Brito ME, Mendonca MG, Gomes YM, Jardim ML, Abath FG (2001) Dynamics of the antibody response in patients with therapeutic or spontaneous cure of American cutaneous leishmaniasis. Trans R Soc Trap Med Hyg 95: 203-206 https://doi.org/10.1016/S0035-9203(01)90168-3
  4. Carmelo E, Martinez E, Gonzalez AC, Pinero JE, Patarroyo ME, Del Castillo A, Valladares B (2002) Antigenicity of Leishmania braziliensis histone H1 during cutaneous leishmaniasis: localization of antigenic determinants. Clin Diagn Lab Immunol 9: 808-811 https://doi.org/10.1128/CDLI.9.1.1-7.2002
  5. Chang KP, Reed SG, McGwire BS, Soong L (2003) Leishmania model for microbial virulence: the relevance of parasite multiplication and pathoantigenicity. Acta Trop 85: 375-390 https://doi.org/10.1016/S0001-706X(02)00238-3
  6. de Oliveira CI, Teixeira MJ, Gomes R, Barral A, Brodskyn C (2004) Animal models for infectious diseases caused by parasites: Leishmaniasis. Drug Discovery Today: Diseases models 1: 81-86 https://doi.org/10.1016/j.ddmod.2004.07.005
  7. Goncalves CC, Reiche EM, De Abreu Filho BA, Silveira TG, Felizardo TC, Maia KR, Costacurta R, Padovesi EJ, Dias Filho BP, Jankevicius SI, Jankevicius JV (2002) Evaluation of antigens from various Leishmania species in a Western blot for diagnosis of American tegumentary leishmaniasis. Am J Trop Med Hyg 66: 91-102 https://doi.org/10.4269/ajtmh.2002.66.91
  8. Gumy A, Louis JA, Launois P (2004) The murine model of infection with Leishmania major and its importance for the deciphering of mechanisms underlying differences in the cell differentiation in mice from different genetic background. Int J Parasitol 34: 433-444 https://doi.org/10.1016/j.ijpara.2003.11.021
  9. Iborra S, Soto M, Carrion J, Alonso C, Requena JM (2004) Vaccination with a plasmid DNA cocktail encoding the nucleosomal histones of Leishmania confers protection against murine cutaneous leishmaniasis. Vaccine 22: 3865-3876 https://doi.org/10.1016/j.vaccine.2004.04.015
  10. Kelly BL, Locksley RM (2004) The Leishmania major LACK antigen with an immunodominant epitope at amino acids 156 to 173 is not required for early Th2 development in BALB/c mice. Infect Immun 72: 6924-6931 https://doi.org/10.1128/IAI.72.12.6924-6931.2004
  11. Lange UG, Mastroeni P, Blackwell JM, Stober CB (2004) DNA-Salmonella enterica serovar Typhimurium primerbooster vaccination biases towards T helper 1 responses and enhances protection against Leishmania major infection in mice. Infect Immun 72: 4924-4928 https://doi.org/10.1128/IAI.72.8.4924-4928.2004
  12. Rafati S, Salmanian AH, Hashemi K, Schaff C, Belli S, Fasel N (2001) Identification of Leishmania major cysteine proteinases as targets of the immune response in humans. Mol Biochem Parasitol 113: 35-43 https://doi.org/10.1016/S0166-6851(00)00377-7
  13. Sacks D, Anderson C (2004) Re-examination of the immunosuppressive mechanisms mediating non-cure of Leishmania infection in mice. Immunol Rev 201: 225-238 https://doi.org/10.1111/j.0105-2896.2004.00185.x
  14. Shimizu Y, Yamakami K, Gomi T, Nakata M, Asanuma H, Tadakuma T, Kojima N (2003) Protection against Leishmania major infection by oligomannose-coated liposomes. Bioorg Med Chem 11: 1191-1195 https://doi.org/10.1016/S0968-0896(02)00644-2
  15. Skeiky YA, Kennedy M, Kaufman D, Borges MM, Guderian JA, Scholler JK, Ovendale PJ, Picha KS, Morrissey PJ, Grabstein KH, Campos-Nero A, Reed SG (1998) LeIF: a recombinant Leishmania protein that induces an IL-12mediated Thl cytokine profile. J Immunol 161: 6171-6179
  16. Tewary P, Sukumaran B, Saxena S, Madhubala R (2004) Immunostimulatory oligodeoxynucleotides are potent enhancers of protective immunity in mice immunized with recombinant ORFF leishmanial antigen. Vaccine 22: 3053-3060 https://doi.org/10.1016/j.vaccine.2004.02.007
  17. Trujillo C, Ramirez R, Velez ID, Berberich C (1999) The humoral immune response to the kinetoplastid membrane protein-ll in patients with American leishmaniasis and Chagas disease: prevalence of IgG subclasses and mapping of epitopes. Immunol Lett 70: 203-209 https://doi.org/10.1016/S0165-2478(99)00146-7