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Oxidative Stability of Lipids from Eel (Anguilla japonica) Fed Conjugated Linoleic Acid

Conjugated Linoleic Acid급여 뱀장어(Anguilla japonica) 지질의 산화안정성

  • Choi, Byeong-Dae (Division of Marine Bioscience/Institute of Marine Industry, Gyeongsang National University) ;
  • Kang, Seok-Joong (Division of Marine Bioscience/Institute of Marine Industry, Gyeongsang National University) ;
  • Ha, Young-Lae (Division of Applied Life Science/Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, So-Young (School of Food Science, Jinju International University) ;
  • Lee, Jae-Jun (Dept. of Food & Nutrition, Chosun University)
  • 최병대 (경상대학교 해양생물이용학부/해양산업연구소) ;
  • 강석중 (경상대학교 해양생물이용학부/해양산업연구소) ;
  • 하영래 (경상대학교 환경생명식품공학부) ;
  • 김소영 (진주국제대학교 식품과학부) ;
  • 이재준 (조선대학교 식품영양학과)
  • Published : 2006.01.01

Abstract

The oxidative stability of lipids from eel (Anguilla japonica) fed diets containing different concentrations of conjugated linoleic acid (CLA) was studied. Eels, 3 weeks of age, with an average weight of 160 g, were randomly divided into 5 groups (5 fishes/group) by body weight, and assigned to one of the five CLA-supplemented diets at the following concentrations: 0, 0.5, 1.0, 2.5, and $5.0\%$ CLA. After 8 weeks of feeding, eels were sacrificed and the total lipid contents were extracted. The lipids from each treatment groups were stored at $37^{\circ}C$ for 5 weeks. Changes in the fatty acid profile, lipid class, weight gained, peroxide value (POV) and carbonyl value (COV) of the lipid from each treatment groups were analyzed weekly. The composition of CLA in the lipids of eels fed with 0.5, 1.0, 2.5, and $5.0\%$ CLA-supplemented diets were 0.5, 1.7, 3.3, and $6.2\%$, respectively After 4 weeks of storage, the proportion of polyunsaturated fatty acids (PUFAs) in the lipid of eels fed diets containing 1.0 and $2.5\%$ CLA were 15.3 and $14.8\%$, respectively. Whereas, lipid extracted from eels fed with 0.5 and $5.0\%$ CLA-supplemented diets contain 11.8 and $7.4\%$ PUFAs, respectively. Lipid from the control sample contained $9.0\%$ PUFAs. POV and COV were found to be the lowest in the lipids samples from 1.0 and $2.5\%$ CLA diets. These results indicate that lipids from diets containing 1.0 or $2.5\%$ CLA were more stable against oxidative rancidity relative to other concentrations, suggesting that these are the appropriate CLA concentrations for the production of stable eel lipids.

최근 건강기능식품에 대한 관심이 높아지면서 기능성을 나타내는 원료에 대한 평가가 이루어지고 있다. 한국인의 식습관 상 어류 섭취가 많고 이로부터 DHA, EPA와 같은 기능성 지질의 섭취도 증가하고 있는 추세에 있다. 본 논문에서는 개별인정 형으로 추진되고 있으며 항암성, 체지방 감소효과 등을 보이고 있는 CLA를 어류사료에 첨가하여 안정성이 높은 중성지질의 형태로 바꾸어 항산화효과를 평가하고자 하였다. 뱀장어 사료에 CLA 함량을 $0\%,\;0.5\%,\;1.0\%,\;2.5\%$, 및 $5.0\%$로 성형하여 순환여과식 사육장치에서 8주간 사육하였다. 사육 후 이들 시료로부터 지질을 추출하고 $37^{\circ}C$에 저장하면서 지방산조성의 변화, 지질 class의 변화, 산소 소비에 따른 중량증가, 과산화물가, 카아보닐가 등으로 항산화능을 측정하였다. 측정결과 CLA 축적량은 $0.5\%,\;1.7\%,\;3.3\%$$6.2\%$로 나타나 CLA 공급이 많을수록 축적되는 CLA량은 증가하였다. 대조구의 경우 저장 3주 후부터 불포화산의 함량이 감소하여 $13.7\%$에서 5주 후에는 $6.2\%$로 급감하였다. $0.5\%$ CLA 급이구는 저장 4주째 불포화산의 함량이 $11.8\%$, 5주째는 $7.4\%$로 감소하였으며, $1.0\%$$2.5\%$ CLA 급이구에서는 불포화산의 함량이 4주째 $15.3\%$$14.8\%$로 변화가 적었다. 중성지질의 class 조성은 $1.0\%$$2.5\%$ CLA 급이구는 저장 5주째 중성지질과 인지질의 비가 53.9:46.1 및 64.3:35.7로 낮아졌으며, 다른 구에서는 저장 4주째부터 산화가 진행되었다. 과산화물가와 카아보닐가의 변화에서도 $1.0\%$$2.5\%$ CLA 급이구가 산화에 가장 안정한 것으로 나타났다.

Keywords

References

  1. Ha YL, Grimm NK, Pariza MW. 1987. Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. Carcinogenesis 8: 1881-1887 https://doi.org/10.1093/carcin/8.12.1881
  2. Ha YL, Storkson J, Pariza MW. 1990. Inhibition of ben-zo[a]pyrene-induced mouse forestomach neoplasia by con-jugated dienoic derivatives of linoleic acid. Cancer Res 50: 1097-1101
  3. Ip C, Chin SF, Scimeca JA, Pariza MW. 1991. Mammary cancer prevention by conjugated dienoic derivative of linoleic acid. Cancer Res 244: 678-6124
  4. Decker EA. 1995. The role of phenolics, conjugated linoleic acid, carnosine, and pyrroloquinoline quinone as nones-sential dietary antioxidants. Nutr Rev 53: 49-58 https://doi.org/10.1111/j.1753-4887.1995.tb01502.x
  5. Chen Z, Chan Y, Kwan KY, Zhang A. 1997. Reassessment of the antioxidant activity of conjugated linoleic acids. J Am Oil Chem Soc 74: 749-753 https://doi.org/10.1007/s11746-997-0213-y
  6. Yurawecz MP, Hood JK, Mossoba MM, Roach JA, Ku Y. 1995. Furan fatty acids determined as oxidation products of conjugated octadecadienoic acid. Lipids 30: 595-598 https://doi.org/10.1007/BF02536995
  7. Szymczyk B, Pisulewski P, Szczurek W, Hanczakowski P. 2000. The effects of feeding conjugated linoleic aicd (CLA) on rat growth performance, serum lipoproteins and sub-sequent lipid composition of selected rat tissues. J Sci Food Agric 80: 1553-1558 https://doi.org/10.1002/1097-0010(200008)80:10<1553::AID-JSFA650>3.0.CO;2-Z
  8. Lawson RE, MossAR, Givens DI. 2000. The role of dairy products in supplying conjugated linoleic acid to man's diet: a review. Nutr Res Rev 14: 153-172 https://doi.org/10.1079/095442201108729178
  9. 김무상. 2003. 어류의 생태. 아카데미서적, 서울. p 316-323
  10. Jeong BY, Choi BD, Moon SK, Lee JS. 1998. Fatty acid com-position of 72 species of Korean fish. J Fish Sci Tech 1: 129-146
  11. Kim YR, Lee YH, Park KA, Kim JO, Ha YL. 2000. A simple method for the preparation of highly pure conjugated li-noleic acid (CLA) synthesized from safflower seed oil. J Food Sci Nutr 5: 10-14
  12. Bligh EG, Dyer WJ. 1959. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37: 911- 917 https://doi.org/10.1139/o59-099
  13. Park SJ, Park CW, Kim SJ, Kim JK, Kim YR, Park KA, Kim JO, Ha YL. 2002. Methylation methods for the quan-titative analysis of conjugated linoleic acid (CLA) isomers in various lipid samples. J Agric Food Chem 50: 989-996 https://doi.org/10.1021/jf011185b
  14. Choi BD, Kang SJ, HA YL, Ackman RG. 1999. Accumu-lation of conjugated linoleic acid (CLA) in tissues of fish fed diets containing various levels od CLA. In Quality attributes of muscle foods. Xiong YL, Ho CT, Shahidi F, eds. Kluwer Academic/Plenum Publishers, New York. p 61-71
  15. Ohshima T, Ackman RG. 1991. New developments in chrom-arod (Iatroscan TLC/FID): Analysis of lipid class composition. J Planar Chromatogr 4: 27-34
  16. Olcott HS, Einset E. 1957. A weighting method for measuring the induction period of marine and other oils. J Am Oil Chem Soc 35: 161-165
  17. AOCS. 1998. Peroxide value, acetic acid-chloroform meth-od. AOCS official method Cd-853. AOCS 15th ed. Champain, Illinois
  18. Henick AS, Bence MF, Mitchell Jr JH. 1954. Estimating carbonyl compounds in rancid fat and foods. J Am Oil Chem Soc 31: 88-91 https://doi.org/10.1007/BF02612488
  19. JMP. 2002. Statistics and graphics guide. Version 5.0. SAS Institute, Cary, NC. p 179-209
  20. Takeuchi T, Watanabe T. 1982. Effects of various polyun-saturated fatty acids on growth and fatty acid compositions of rainbow trout Salmo gairdneri, Coho salmon On-corhynchus kisutch and chum salmon Oncorhynchus keta. Nippon Suisan Gakkaishi 48: 1745-1752 https://doi.org/10.2331/suisan.48.1745
  21. Yang L, Leung LK, Huang Y, Chen Z. 2000. Oxidative stability of conjugated linoleic acid isomers. J Agric Food Chem 48: 3072-3076 https://doi.org/10.1021/jf0003404
  22. Yang L, Ying C, Chen ZY. 2004. Stability of conjugated linoleic acid isomers in egg yolk lipids during frying. Food Chem 86: 531-535 https://doi.org/10.1016/j.foodchem.2003.09.006
  23. Kim HY, Shin JW, Sim GC, Park HO, Kim HS, Kim SM, Cho JS, Jang YM. 2000. Comparison of the taste compounds of wild and cultured eel, puffer and snake head. Korean J Food Sci Technol 32: 1058-1067
  24. Ackman RG. 1995. Composition and nutritional value of fish and shellfish lipids. In Fish and fishery products. Ruiter A, ed. CAB International, Wallingford, Oxon. p 117-156
  25. Yang L, Huang Y, James AE, Lam LW, Chen ZY. 2002. Different incorporation of conjugated linoleic acid isomers into egg yolk lipids. J Agric Food Chem 50: 4941-4946 https://doi.org/10.1021/jf020363d
  26. Chen ZY, Nawar WW. 1991. Prooxidative and antioxidative effects of phospholipids on milk fat. J Am Oil Chem Soc 68: 938-940 https://doi.org/10.1007/BF02657539
  27. Jeong BY, Ryu SN, Hur HS. 1997. Antioxidant effect of sesame lignins on $\alpha$-linoleic acid-concentrated perilla fatty acid esters. J Korean Soc Food Sci Nutr 26: 1033-1038
  28. Takeuchi M, Hara S, Totani Y, Hibino H, Tanaka Y. 1997. Autoxidative behavior polyunsaturated phospholipids. I. Oxidative stability of marine oil containing polyunsaturated phospholipids. Japan Oil Chem Soc 46: 175-181 https://doi.org/10.5650/jos1996.46.175

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