Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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High-Level Production of Low-Branched Levan from Pseudomonas aurantiaca S-4380 for the Production of $di-\beta-D-Fructofuranose$ Dianhydride IV

  • JANG KI-HYO (Department of Food and Nutrition, Samcheok National University) ;
  • JANG EUN-KYUNG (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology) ;
  • KIM SEUNG-HWAN (Korea Food and Drug Administration) ;
  • KIM IN-HWAN (RealBioTech Co. Ltd.) ;
  • KANG SOON AH (Department of Fermented Food Science, Seoul University of Venture & Information) ;
  • KOH ISSAC (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology) ;
  • PARK YOUNG-IL (Division of Biotechnology, The Catholic University of Korea) ;
  • KIM YOUNG-JUN (Division of Biotechnology, The Catholic University of Korea) ;
  • HA SANG-DO (Department of Food Science and Technology, Chung-Ang University) ;
  • KIM CHUL HO (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2006.01.01
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Abstract

The IscA gene, encoding a levansucrase of 424 amino acids (aa) residues, was cloned from the genomic DNA of Pseudomonas aurantiaca S-4380, and overexpressed in Escherichia coli. The recombinant levansucrase overexpressed in E. coli was then used to produce levan from sucrose. Levan crystals with 98% purity could be obtained from the reaction mixture with $62\%$ yield using an alcohol precipitation method. The molecular weight of the levan was $7\times10^5$ daltons. Methylation studies showed that the levan was branched: main linkage C-2,6; branched linkage C-2,1; and degree of branching $6\%$. Three bacterial levans from different strains were incubated with levan fructotransferase (LFTase) from Arthrobacter ureafaciens K2032, which produced $di-\beta-D-fructofuranose$ dianhydride IV (DFA IV); final conversion yields from the levans to DFA IV were $39\%$ in Zymomonas mobilis, $53\%$ in Serratia levanicum, and $59\%$ in P. aurantiaca S-4380 levansucrase. The levan from P. aurantiaca S-4380 levansucrase gave the highest conversion yield of levan to DFAIV so far reported.

Keywords

References

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