Journal of Microbiology and Biotechnology

  • 제16권1호
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  • Pages.64-67
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  • 2006
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Enzymatic Synthesis of Ascorbic Acid Fructoside by Transfructosylation Using Levan Fructotransferase

  • LEE CHOONG YEUL (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • KIM KI HO (R&D Center, Bioland Ltd.,) ;
  • HUR SUN YEON (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • HEO JOO-HYUNG (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • CHOI MIN HO (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • RHEE SANG KI (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • KIM CHUL HO (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
  • 발행 : 2006.01.01
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초록

To enhance the stability of ascorbic acid, the glycosylation of ascorbic acid was studied using the transfructosylation activity of levan fructotransferase. When levan was used as glycosyl donor, a novel fructoside (ascorbic acid 2-ffuctoside) was formed by the transfructosylation activity of the levan fructotransferase. The production of ascorbic acid 2-fructoside was highly affected by the concentration of the fructosyl acceptor (ascorbic acid). When $35\%$ of ascorbic acid and $2\%$ of levan were incubated with LFTase of 0.5 unit/glevan at $37^{\circ}C$ for 85 h, a maximum 52 g/l of AA-2F was produced.

키워드

참고문헌

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