Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enzymatic Synthesis of Ascorbic Acid Fructoside by Transfructosylation Using Levan Fructotransferase

  • LEE CHOONG YEUL (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • KIM KI HO (R&D Center, Bioland Ltd.,) ;
  • HUR SUN YEON (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • HEO JOO-HYUNG (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • CHOI MIN HO (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • RHEE SANG KI (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • KIM CHUL HO (Laboratory of Metabolic Engineering, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
  • Published : 2006.01.01
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Abstract

To enhance the stability of ascorbic acid, the glycosylation of ascorbic acid was studied using the transfructosylation activity of levan fructotransferase. When levan was used as glycosyl donor, a novel fructoside (ascorbic acid 2-ffuctoside) was formed by the transfructosylation activity of the levan fructotransferase. The production of ascorbic acid 2-fructoside was highly affected by the concentration of the fructosyl acceptor (ascorbic acid). When $35\%$ of ascorbic acid and $2\%$ of levan were incubated with LFTase of 0.5 unit/glevan at $37^{\circ}C$ for 85 h, a maximum 52 g/l of AA-2F was produced.

Keywords

References

  1. Aga, H., M. Yoneyama, S. Sakai, and I. Yamamoto. 1991. Synthesis of 2-O-${\alpha}$-D-glucopyranosyl L-ascorbic acid by cyclomaltodextrin glucanotransferase from Bacillus stearothermophilus. Agric. Biol. Chem. 55: 1751-1756 https://doi.org/10.1271/bbb1961.55.1751
  2. Kharel, K. L., Kwang, J. K. Sohng, H. C. Lee, and M. Kumar. 2004. Production of dTDP-4-keto-6-deoxy- D-glucose by immobilization of dTDP-D-glucose 4,6- dehydratase. J. Microbiol. Biotechnol. 14: 297-302
  3. Kim, H. J., H. E. Park, M. J. Kim, H. G. Lee, J. Y. Yang, and J. H. Cha. 2003. Enzymatic characterization of a recombinant levansucrase from Rahnella aquatilis ATCC 15552. J. Microbiol. Biotechnol. 13: 230-236 https://doi.org/10.1159/000075836
  4. Kim, C. H., K. B. Song, S. K. Rhee, B. Y. Kim, and U. H. Chun. 1998. Viscosity of levan produced by levansucrase from Zymomonas mobilis. Food Eng. Prog. 2: 217-222
  5. Mandi, T., M. Yoneyama, and S. Sakai. 1995. Process for preparing high 2-O-${\alpha}$-D-glucopyranosyl-L-ascorbic acid content product. United States Patent 5,468,850
  6. Muto, N., S. Suga, K. Fujii, K. Goto, and I. Yamamoto. 1990. Formation of a stable ascorbic acid 2-glucoside by specific transglucosylation with rice seed ${\alpha}$-glucosidase. Agric. Biol. Chem. 54: 1697-1703 https://doi.org/10.1271/bbb1961.54.1697
  7. Park, P. J., H. K. Lee, and S. K. Kim. 2004. Preparation of hetero-chitooligo-saccharides and their antimicrobial activity on Vibrio parahaemolyticus. J. Microbiol. Biotechnol. 14: 41-48
  8. Ramalingam, G. and V. H. Mulimani. 2004. Purification and characterization of guar galactomannan degrading ${\alpha}$- galactosidase from Aspergillus oryzae DR-5. J. Microbiol. Biotechnol. 14: 863-865
  9. Rhee, S. K., K. B. Song, C. H. Kim, B. S. Park, E. K. Jang, and K. H. Jang. 2002. Levan, pp. 351-377. In: Vandamme, E. J., De Baets, S. and Steinbuchel, A., (eds.) Biopolymers, Vol. 5. Weinheim: Wiley-VCH Verlag GmbH
  10. Somogyi, M. 1952. Notes on sugar determination. J. Biol. Chem. 195: 19-23
  11. Song, K. B., K. S. Bae, Y. B. Lee, K. Y. Lee, and S. K. Rhee. 2000. Characteristics of levan fructotransferase from Arthrobacter ureafaciens K2032 and difructose anhydride IV formation from levan. Enzyme Microb. Technol. 27: 212-218 https://doi.org/10.1016/S0141-0229(00)00135-6
  12. Tanaka, M., N. Muto, and I. Yamamoto. 1991. Characterization of Bacillus stearothermophilus cyclodextrin glucanotransferase in ascorbic acid 2-O-${\alpha}$-glucoside formation. Biochim. Biophys. Acta. 1078: 127-132 https://doi.org/10.1016/0167-4838(91)99000-1
  13. Yamamoto, I., N. Muto, E. Nagata, T. Nakamura, and Y. Suzuki. 1990. Formation of a stable L-ascorbic acid ${\alpha}$-glucoside by mammalian ${\alpha}$-glucosidase-catalyzed transglucosylation. Biochim. Biophys. Acta. 1035: 44-50 https://doi.org/10.1016/0304-4165(90)90171-R