Journal of Microbiology and Biotechnology

  • 제16권1호
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  • Pages.32-36
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  • 2006
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Screening from the Genome Databases: Novel Epoxide Hydrolase from Caulobacter crescentus

  • HWANG SEUNGHA (School of Chemical and Biological Engineering, College of Engineering, Seoul National University) ;
  • HYUN HYEJIN (Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University) ;
  • LEE BYOUNGJU (Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University) ;
  • PARK YOUNGSEUB (Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University) ;
  • CHOI CHAYONG (School of Chemical and Biological Engineering, College of Engineering, Seoul National University, Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University) ;
  • HAN JIN (Department of Molecular Physiology and Biophysics, College of Medicine, Inje University) ;
  • JOO HYUN (Department of Molecular Physiology and Biophysics, College of Medicine, Inje University)
  • 발행 : 2006.01.01
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초록

The genome sequences from several microbes have led to the discovery of numerous open reading frames of unknown functionality. The putative bacterial epoxide hydrolase (EH) genes selected from the genome databases were examined for their activities toward various epoxides. Among the nine open reading frames (ORFs) from four microbial species, the ORF from Caulobacter crescentus showed an epoxide hydrolase activity. The kinetic resolution, using C. crescentus EH (CCEH) of the aryl epoxides such as styrene oxide, could be performed more efficiently than short aliphatic epoxides. The resolution of racemic indene oxide, which could previously be resolved only by fungal epoxide hydrolases, was effectively accomplished by CCEH.

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참고문헌

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