Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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In vivo Functional Analysis of γ-butyrolactone Autoregulator Receptor Gene (scaR) in Streptomyces clavuligerus

Streptomyces clavuligerus의 γ-butyrolactone autoregulator receptor 유전자에 대한 in vivo 기능 분석

  • Kang Su-Jin (Division of Food Science and Biotechnology, Kyungnam University) ;
  • Lee Chang-Kwon (Department of Physiology, College of Medicine, Institute of Biomedical Science and Technology, Konkuk University) ;
  • Choi Sun-Uk (Division of Food Science and Biotechnology, Kyungnam University) ;
  • Kim Hyun-Soo (Department of Microbiology, Keimyung University) ;
  • Hwang Yong-Il (Division of Food Science and Biotechnology, Kyungnam University)
  • 강수진 (경남대학교 식품생명공학부) ;
  • 이창권 (건국대학교 의학전문대학원 생리학교실) ;
  • 최선욱 (경남대학교 식품생명공학부) ;
  • 김현수 (계명대학교 미생물학과) ;
  • 황용일 (경남대학교 식품생명공학부)
  • Published : 2006.02.01
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Abstract

A $\gamma-butyrolactone$ autoregulator receptor has a common activity as DNA-binding transcriptional repressors controlling secondary metabolism and/or morphological differentiation in Streptomyces. A gene (scaR) encoding it was cloned from Streptomyces cravuligerus, a clavulanic acid producer, and was in vitro characterized in a previous report. In this study to clarify the in vivo function of ScaR, a $\gamma-butyrolactone$ autoregulator receptor of Streptomyces clavuligerus, we constructed a scaR-deleted strain by means of homologous recombination. No difference in morphology was found between the wild-type strain and the scaR-disruptant, but the scaR-disruptant showed higher clavulanic acid production. This indicates that the ScaR in S. clavuligerus acts as a negative regulator of the biosynthesis of clavulanic acid, but plays no role in morphological differentiation.

방선균에서 DNA 전사 억제인자로써 작용하여 이차대사산물의 생산뿐만 아니라 형태분화를 조절하는 $\gamma-butyrolactone$ autoregulator receptor를 암호화하는 유전자(scaR)를 clavulanic arid 생산 균주, Streptomyces clavuligerus로부터 클로닝하고 in vitro에서 ScaR의 특징을 연구하여 보고한 바 있다. 따라서 본 연구에서는 ScaR의 in vivo 기능을 분석하기위해 상동재조합방법을 이용하여 scaR이 제거된 변이주를 제작하고 야생균주와 표현형을 비교해 보았다. 그 결과, Streptomyces clavuligerus의 형태분화에서는 큰 차이를 나타내지 않았지만 clavulanic acid의 생산에 있어서는 scaR 파괴 변이주가 야생균주에 비해 생산이 증가된 경향을 보였다. 그러므로 ScaR이 S. clavuligerus의 형태분화에는 영향을 미치지 않지만 clavulanic acid 생합성에는 negative regulator로 작용한다는 것을 본 연구를 통해 명확하게 확인할 수 있었다.

Keywords

References

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