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Bacillus stearothermophilus DL-3을 사용하여 제조한 미생물 제재가 닭과 돼지의 성장에 미치는 영향

Effect of Microbial Products Made of Bacillus stearothermophilus DL-3 on Growth of Chickens and Pigs

  • 김순희 (동아대학교 생명자원과학대학 응용생물학) ;
  • 조강익 (동아대학교 생명자원과학대학 생명공학) ;
  • 이유정 (동아대학교 생명자원과학대학 생명공학) ;
  • 오주성 (동아대학교 생명자원과학대학 원예식물생명공학) ;
  • 정순재 (동아대학교 생명자원과학대학v원예식물생명공학) ;
  • 문병주 (동아대학교 생명자원과학대학 응용생물학) ;
  • 강경희 (농촌진흥청 원예연구소 시설원예시험장) ;
  • 이진우 (동아대학교 생명자원과학대학 생명공학)
  • Kim Soon-Hee (Department of Applied Biology, College of Nature Resources and Life Science, Dong-A University) ;
  • Jo Kang-Ik (Department of Biotechnology, College of Nature Resources and Life Science, Dong-A University) ;
  • Lee You-Jung (Department of Biotechnology, College of Nature Resources and Life Science, Dong-A University) ;
  • Oh Ju-Sung (Horticultural Plant Biotechnology, College of Nature Resources and Life Science, Dong-A University) ;
  • Jung Soon-Jae (Horticultural Plant Biotechnology, College of Nature Resources and Life Science, Dong-A University) ;
  • Moon Byung-Ju (Department of Biotechnology, College of Nature Resources and Life Science, Dong-A University) ;
  • Kang Kyung-Hee (National Horticultural Research Institute, Protected Horticulture Experiment Station) ;
  • Lee Jin-Woo (Department of Biotechnology, College of Nature Resources and Life Science, Dong-A University)
  • 발행 : 2006.02.01

초록

유기물이 풍부한 토양에서 분리하여 동정한 Bucillus stearothermophilus DL-3균주의 배양액과 사과박, 간장박 및 미강 등을 사용하여 2종류의 미생물 제재를 제조하고 닭과 돼지의 생육에 미치는 영향을 조사하였다. 사과박의 주성분은 탄수화물이며 간장박의 주성분은 단백질이다. B. stearothermophilus DL-3 균주의 배양액과 미강을 사용하여 제조한 미생물 제제를 미생물 제제 A로 명명하고 B. stearothermophilus DL-3균주의 배양액, 사과박, 간장박 및 미강을 사용하여 제조한 미생물 제제를 미생물 제제 B로 명명하였다. 미생물 제재가 닭 및 돼지의 생육에 미치는 영향을 검토하기 위하여 일반사료를 급여한 구, 일반사료의 $10\%$를 미생물 제재 A로 대체하여 급여한 구 및 일반사료의 $10\%$를 미생물 제재 B로 대체하여 급여한 구로 나누어 효과 검정실험을 수행하였다. 실험에 사용한 각 구의 평균 병아리 무게는 각각 $41.1{\pm}2.5g,\;41.6{\pm}3.2g$$42.3{\pm}2.9g$이었다. 급여를 시작하지 28일 후, 각 구에 속한 병아리들의 평균 무게는 각각 $547.7{\pm}91.7g,\;560.1{\pm}17.2g$$562.2{\pm}32.5g$이었으며 각 구에 속한 병아리들의 평균 무게 증가는 각각 506.6 g, 518.5 g 및 519.9 g이었다. 즉, 일반사료의 $10\%$를 미생물 제재 A로 대체하여 급여한 군과 일반사료의 $10\%$를 미생물 제재 B로 대체하여 급여한 군은 일반사료를 급여한 군에 비하여 각각 $2.4\%$$2.6\%$의 무게 증가를 나타났다. 각 구의 평균 돼지 무게는 각각 $9.3{\pm}1.0kg,\;9.4{\pm}1.1kg$$9.6{\pm}1.0kg$이었다. 급여를 시작한지 35일 후, 각 구에 속한 돼지들의 평균 무게는 각각 $19.3{\pm}4.1kg,\;20.2{\pm}3.9kg$$20.8{\pm}4.2kg$이었으며 각 구에 속한 돼지들의 평균 무게 증가는 각각 10.7 kg, 10.8 kg 및 11.2 kg이었다. 닭의 사료 효과 검정 결과와 같이 일반사료의 $10\%$를 미생물 제재 A로 대체하여 급여한 군과 일반사료의 $10\%$를 미생물 제재 B로 대체하여 급여한 군은 일반사료를 급여한 군에 비하여 각각 $1.6\%$$5.2\%$의 무게 증가를 나타났다.

Effect of microbial products made of Bacillus stearothermophilus DL-3 on growth of chickens and pigs was investigated. Two types of microbial product were made in this study. One is the microbial product made of culture broth of B. stearothemophilus DL-3 and rice bran which named as the microbial product A. The other is the microbial product made of culture broth of B. stearothermophilus DL-3, apple pomace, soybean pomace and rice bran which named as the microbial product B. Chickens were divided into three groups and each group was fed with $100\%$ general feed, $90\%$ general feed supplemented with $10\%$ microbial product A or $90\%$ general feed supplemented with $10\%$ microbial product B. The average chicken weight of each group was $41.1{\pm}2.5g,\;41.6{\pm}3.2g\;and\;42.3{\pm}2.9g$ and those after 28 days was $547.7{\pm}91.7g,\;560.1{\pm}17.2g\;and\;562.2{\pm}32.5g$, respectively. The average weight gain for each group was 506.6 g, 518.6 g and 519.9 g, respectively, and weight increases of groups fed with $90\%$ general feeder and $10\%$ microbial product A and B were $2.4\%\;and\;2.6\%$ higher than the group fed with $100\%$ general feed. Pigs were also divided into three groups and each group was fed like chickens. The average weight of each group was $9.3{\pm}1.0kg,\;9.4{\pm}1.1kg\;and\;9.6{\pm}1.0kg$ and those after 37 days was $19.3{\pm}4.1kg,\;20.2{\pm}3.9kg\;and\;20.8{\pm}4.2kg$, respectively. The average Weight gain for each group was 10.65 kg, 10.82 kg and 11.20 kg, respectively, and weight increases of groups fed with $90\%$ general feeder and $10\%$ microbial product A and B were $1.6\%$ and $5.2\%$ higher than the group fed with $100\%$ general feed.

키워드

참고문헌

  1. Azizah, A. H. and Y. S. Luan. 2000. Functional properties of dietary fibre prepared from defatted rice bran. Kor. J. Food Chem. 68, 15-19 https://doi.org/10.1016/S0308-8146(99)00145-4
  2. Elad, Y., I. Chet and Y. Henis. 1982. Degradation of plant pathogenic fungi by Tricoderma harzianum. Can. J. Microbiol. 28, 719-725 https://doi.org/10.1139/m82-110
  3. Gimeno-Garcia, E., V. Andreu and R. Boluda. 1996. Heavy metals incidence in the application of inorganic fertilizers and pesticides to rice farming soils. Environ. Pollu. 92, 19-25 https://doi.org/10.1016/0269-7491(95)00090-9
  4. Hepola, H. 2003. Milk feeding systems for dairy calves in groups: effects of feed intake, growth and health. App. Animal Behav. 80, 233-243 https://doi.org/10.1016/S0168-1591(02)00214-9
  5. Hong, J. W., I. H. Kim, O. S. Kwon, S. H. Lee and C. S. Kim. 2001. Development of finishing pig diet for friendly environment by using slaughter poultry waste product. Kor. J. Animal Sci. & Technol. 43, 75-84
  6. Jo, I. H., B. S. Hwang, J. H. Ahn, and J. S. Lee, 1999. Use of apple pomace and rice bran of agricultural by-products for the development of diets of Korean native growing goats. Kor. J. Animal Nutr. Feed. 23, 327-334
  7. Joshi, V. K. and D. K. Sandhu. 1996. Preparation and evaluation of an animal feed byproduct produced by solid-state fermentation of apple pomace. Biores. Technol. 56, 351-255
  8. Kim, J. M., C. S. Kim, H. J. Kim, B. J. Moon, J. H. Lee, D. S. Lee and J. w. Lee. 2002. Effect of microbial product on microorganisms in soli and growth of cabbage and tomato. Kor. J. Life Sci. 12, 515-522 https://doi.org/10.5352/JLS.2002.12.5.515
  9. Kim, S. H., K. S. Bae, J. K. Yang, Y. J. Lee, J. S. Oh, S. J. Jung, B. J. Moon, J. W. Lee. 2004. Effect of microbial product made of Bacillus stearothermophilus DL-3 on microorganisms in soil and growth of lettuce and Chinese cabbage. Kor. J. Life Sci. 14, 778-787 https://doi.org/10.5352/JLS.2004.14.5.778
  10. Kim, S. J. and S. K. Shin. 1997. Effects of bioremediation products on the oil degradability. Kor. J. Microbiol. 33, 157-162
  11. Lapdot, A., A. Mechaly, and Y. Shoham. 1996. Overexpression and single-step purification of a thermostable xylanase fron Bacillus stearothermophilus T-6. J. Biotechnol. 51, 259-264 https://doi.org/10.1016/S0168-1656(96)01604-5
  12. Lee, H. S., S. G. Cho and Y. J. Choi. 1996. Pentose utilization by the xylanolytic bacterium Bacillus stearothermophilus. Kor. J. Appl. Microbiol. Biotechnol. 24, 385-92
  13. Lee, J. H., Y. C. Kim, M. Y. Kim, H. S. Chung and S. K. Chung. 2000. Antioxidative activity and related compounds of apple pomace. Kor. J. Food Sci. Technol. 32(4), 908-913
  14. Lee, K. B., Y. W. Kim and K. S. Kim. 1988. Effects of pesticides on microorganisms related to the nitrogen cycle in the submerged soil. Kor. J. Soil Fer. 21, 149-159
  15. Park, S. K. and S. H. Baek. 2000. Effect of yeast extracts concentration on cell growth and products of Bacillus stearothermophilus. Kor. J. Sci. & Nat. Res. 22, 41-54
  16. Raghukumar, C., V. Vipparty, J. J. David and D. Chandramohan. 2001. Degradation of crude oil by marine cyanobacteria. Appl. Microbiol. Biotechnol. 57, 433-436 https://doi.org/10.1007/s002530100784
  17. Simoes, D. C., M. D. McNeil, B. Kristiansen and M. Mattey. 1997. Purification and partial characterization of a 1.57 kDa thermostable esterase from Bacillus stearothermophilus. FEMS Microbiol. Lett. 147, 151-156 https://doi.org/10.1111/j.1574-6968.1997.tb10235.x
  18. Song, H. S. and Y. J. Choi. 1989. Production of xylanase by Bacillus stearothemophilus. Kor. J. Appl. Microbial. Bioeng. 17, 289-294
  19. Sookkheo, B., S. Sinchaikul, S. Phutrakul and S-T. Chen. 2000. Purification and characterization of the highly thermostable proteases from Bacillus stearothermophilus TLS33. Protein Exp. Puri. 20, 142-151 https://doi.org/10.1006/prep.2000.1282
  20. Suh, J. S. and J. S. Shin. Soil microbial diversity of paddy fields in Korea. Kor. J. Soil. Fert. 30, 200-207
  21. Trewavas, A. 2001. Urban myths of organic farming. Nature 410, 409-410 https://doi.org/10.1038/35068639