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사람 위 상피세포의 염증반응에 대한 무의 효과

Effect of Radish on Inflammatory Reaction in Human Epithelial Gastric Cell

  • 손윤희 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소) ;
  • 정유선 (동국대학교 의과대학 내과학교실) ;
  • 서정일 (동국대학교 의과대학 내과학교실) ;
  • 박인경 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소) ;
  • 남경수 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소)
  • Shon, Yun-Hee (Department of Pharmacology, College of Medicine and Intractable Disease Research Center) ;
  • Chung, Yoo-Sun (Department of Internal Medicine, College of Medicine Dongguk University) ;
  • Suh, Jeong-Ill (Department of Internal Medicine, College of Medicine Dongguk University) ;
  • Park, In-Kyung (Department of Pharmacology, College of Medicine and Intractable Disease Research Center) ;
  • Nam, Kyung-Soo (Department of Pharmacology, College of Medicine and Intractable Disease Research Center)
  • 발행 : 2006.02.01

초록

The effects of Korean and Japanese radishes on inflammatory reaction that involves arachidonic acid cascades were investigated in human epithelial gastric cell. The activities of type I (porcine pancreas) and type II (Crotalus atrox) phospholipase $A_2(PLA_2$) were inhibited by radish. Cyclooxygenase-2 (COX-2) activity was significantly suppressed by radish (p<0.05, p<0.01 and p<0.005). The nitric oxide production was also inhibited by radish. The Korean radish was more effective in inhibition of $PLA_2$ and COX-2 activities and nitric oxide production than Japanease radish. These results indicate that radish has a protective effect on gastric epithelial cell inflammation by suppressing the activities of $PLA_2$ and COX-2 activities and nitric oxide production from gastric epithelial cell.

키워드

참고문헌

  1. Ambs, S., W. G. Merriam, W. P. Bennett, E. Felley-Bosco, M. O. Ogunfusika, S. M. Oser, S. Klein, P. G. Shields, T. R. Billiar and C. C. Harris. 1998. Frequent nitric oxide synthase-2 expression in human colon adenomas: implication for tumor angiogenesis and colon cancer progression. Cancer Res. 58, 334-341
  2. Bajt, M. L., T. R. Knight, A. Farhood and H. Jaeschke. 2003. Scavenging peroxynitrite with glutathione promotes regeneration and enhances survival during acetaminophen-induced liver injury in mice. J. Pharmacol. Exp. Ther. 307, 67-73 https://doi.org/10.1124/jpet.103.052506
  3. Bing, R. J., M. Miyataka, K. A. Rich, N. Hanson, X. Wang, H. D. Slosser and S. R. Shi. 2001. Nitric oxide, prostanoids, cyclooxygenase, and angiogenesis in colon and breast cancer. Clin. Cancer Res. 7, 3385-3392
  4. Dennis, E. A. 1987. Phospholipase $A_2$ mechanism: Inhibition and role in arachidonic acid release. Drug Development Research 10, 205-220 https://doi.org/10.1002/ddr.430100404
  5. Dole, V. P. and H. Meiner. 1960. Microdetermination of long chain fatty acids in plasma and tissues. J. Biol. Chem. 235, 2595-2599
  6. Farinati, F., F. Valiante, B. Germana, G. Della Libera, R. Baffa, M. Rugge, M. Plebani, F. Vianello, F. Di Mario and R. Naccarato. 1993. Prevalence of Helicobacter pylori infection in patients with precancerous changes and gastric cancer. Eur. J. Cancer Prev. 2, 321-326 https://doi.org/10.1097/00008469-199307000-00006
  7. Kawasaki, K., R. S. Jr. Smith, C. M. Hsieh, J. Sun, J. Chao and J. K. Liao. 2003. Activation of the phosphatidylinositol 3-kinase/protein kinase Akt pathway mediates nitric oxide-induced endothelial cell migration and angiogenesis. Mol. Cell. Biol. 23, 5726-5737 https://doi.org/10.1128/MCB.23.16.5726-5737.2003
  8. Kim, J. S., J. M. Kim, H. C. Jung and I. S. Song. 2001. Expression of cyclooxygenase-2 in human neutrophils activated by Helicobacter pylori water-soluble proteins: possible involvement of $NF-_{\kappa}B$ and MAP kinase signaling pathway. Dig. Dis. Sci. 46, 2277-2284 https://doi.org/10.1023/A:1011939704802
  9. Kim, J. M., J. S. Kim, H. C. Jung, I. S. Song and C. Y. Kim. 2000. Apoptosis of human gastric epithelial cells via caspase-3 activation in response to Helicobacter pylori infection: possible involvement of neutrophiles through tumor necrosis factor-alpha and soluble Fas ligands. Scand. J. Gastroenterol. 35, 40-48 https://doi.org/10.1080/003655200750024515
  10. Konturek, S. J., P. C. Kontruek, M. Plonka, A. Duda, E. Sito, M. Zuchowicz and E. G. Hahn. 2001. Implication of gastrin in cyclooxygenase expression in Helicobacter pylori-infected gastric ulceration. Prostaglandins other Lipid Mediators 66, 39-51 https://doi.org/10.1016/S0090-6980(01)00142-3
  11. Konturek, P. C., K. Rembiasz, S. J. Konturek, J. Stachura, W. Jielanski, K. Galuschka, D. Karcz and E. G. Hahn. 2003. Gene expression of ornithine decarboxylase, cyclooxygenase-2 and gastrin in atrophic gastric mucosa infected with Helicobacter pylori before and after eradication therapy. Dig. Dis. Sci. 48, 36-46 https://doi.org/10.1023/A:1021774029089
  12. Mukherjee, A. B., L. Miele and N. Pattabiraman. 1994. Phospholipase $A_2$ enzymes : regulation and physiological role. Biochem. Pharmacol. 48, 1-10 https://doi.org/10.1016/0006-2952(94)90216-X
  13. Nomura, A., G. N. Stemmermann, P. H. Chyou, G. I. Perez-Perez and M. J. Blaser. 1991. Helicobacter pyroli in fection and gastric carcinoma in a population of Japanese-Americans in Hawaii. N. Engl. J. Med. 325, 1132-1136 https://doi.org/10.1056/NEJM199110173251604
  14. Nomura, A., G. N. Stemmermann, P. H. Chyou, I. Kato, G. I. Perez-Perez and M. J. Blaser. 1994. Helicobacter pylori infection and the risk for duodenal and gastric ulceration. Ann. Intern. Med. 120, 977-981 https://doi.org/10.7326/0003-4819-120-12-199406150-00001
  15. Pruzanski, W. and P. Vadas. 1991. Phospholipase $A_2-a$ mediator between proximal and distal effectors of inflammation. Immunol. Today 12, 143-146
  16. Ristimaki, A., N. Honkanen, H. Jankala, P. Sipponen and M. Harkonen. 2002. Expression of cyclooxgenase-2 in human gastric carcinoma. Cancer Res. 57, 1276-1280
  17. Shon, Y. H., J. I. Surh, Y. J. Chung, I. K. Park, H. C. Kim, C. W. Hwang, C. H. Kim and K. S. Nam. 2004. Effect of radish on HeLa cell vacuolation induced by Helicobacter pylori cytotoxin. Kor. J. Pharmacogn. 35, 250-254
  18. Shon, Y. H., J. I. Surh, I. K. Park, H. C. Kim, C. W. Hwang, C. H. Kim and K. S. Nam. 2005. Inhibitory effect of radish on gastric cell toxicity and interleukin-8 production induced by Helicobacter pylori. J. Life Science 15, 595-599 https://doi.org/10.5352/JLS.2005.15.4.595
  19. Surh, Y. J., K. S. Chun, H. H. Cha, S. S. Han, Y. S. Keum, K. K. Park and S. S. Lee. 2001. Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kappa B activation. Mutat. Res. 480/481, 243-268 https://doi.org/10.1016/S0027-5107(01)00183-X

피인용 문헌

  1. Anti-inflammatory Effect and Antioxidative Activities of Ingredients used in Bibimbab vol.23, pp.2, 2013, https://doi.org/10.5352/JLS.2013.23.2.213