YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effects of Dipsacus asper on Free Radical Scavenging and Lowdensity Lipoprotein Oxidation

속단의 유리기소거활성 및 저밀도지방단백질 산화에 미치는 영향

  • Park Hae-Sun (College of Pharmacy, Sookmyung Women's University) ;
  • Yang Ki-Sook (College of Pharmacy, Sookmyung Women's University)
  • 박혜선 (숙명여자대학교 약학대학) ;
  • 양기숙 (숙명여자대학교 약학대학)
  • Published : 2006.02.01
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Abstract

The roots of Dipsacus asper are used in traditional Chinese medicine as an analgesic, enhancement of liver activity, an anti-inflammatory agent and the treatment of fractures. In order to investigate the antioxidative activity, Dipsaci Radix was extracted with MeOH and fractionated with $CHCl_3$, EtOAc, BuOH and water. MeOH ex. and its solvent fractions were determined on the free radical scavenging activity by DPPH method and antioxidative activity on low density lipoprotein oxidation. The EtOAc and BuOH fractions showed antioxidative activities and from their fractions, two compounds were isolated and identified as hederagenin 3-O-${alpha}$-L-arabino pyranoside (compound 1) and O-${\alpha}$-L-arabinopyranosyl hed-eragenin 28-O-${\beta}$-D-glucopyranosyl ($1{\to}6$)-${\beta}$-D-glucopyranosyl ester (compound 2). Saponin glycoside, compound I showed anti-oxidative activity.

Keywords

References

  1. 이상인, 안덕균, 신민교, 노승현, 이영종, 김선희 : 한약임상응용, 성보사, 서울 p. 346 (1986)
  2. 진존인 : 도설 한방의약대사전 (중국약학대전), 동도문화사, 서울 p.376 (1984)
  3. Kouno, I., Tsuboi, A., Nanri, M. and Kawano, M. : Arylated triterpene glycoside from the roots of Dipsacus asper. Phytochem. 29, 338 (1990) https://doi.org/10.1016/0031-9422(90)89068-K
  4. Jung, K. Y., Do, J. C. and Son, K. H. : Triterpene glycosides from the roots of Dipsacus asper. J. Nat. Prod. 56, 1912 (1993) https://doi.org/10.1021/np50101a007
  5. Tomita, H. and Mouri, Y. : An iridoid glucoside from Dipsacus asperoides. Phytochem. 42, 239 (1996) https://doi.org/10.1016/0031-9422(95)00904-3
  6. Jung, K. Y. : Studies on the terpenoid constituents from the roots of Dipsacus asper. Ph.D. Thesis, Yeungnam University (1995)
  7. Oh, S. R., Jung, K. Y., Son, K. H., Park, S. H., Lee, I. S., Ahn, K. S. and Lee, H. K. : In vitro anticomplementary activity of hederagenin saponins isolated from root of Dipsacus asper. Arch. Pharm. Res. 22, 317 (1999) https://doi.org/10.1007/BF02976371
  8. Suh, H. W., Song, D. K., Son, K. H., Wie, M. B., Lee, K. H., Jung, K. Y., Do, J. C. and Kim, Y. H. : Antinociceptive mechanisms of Dipsacus saponin C administered intracerebroventricularly in the mouse. Gen. Pharmac. 27, 1167 (1996) https://doi.org/10.1016/S0306-3623(96)00052-3
  9. Suh, H. W., Song, D. K., Huh, S. O., Son, K. H. and Kim, Y. H. : Antinociceptive mechanisms of Dipsacus saponin C administered intrathecally in the mouse. J. Ethnopharmacol. 71, 211 (2000) https://doi.org/10.1016/S0378-8741(99)00204-4
  10. Hung, T. M., Jin, W. Y., Thuong, P. T., Song, K. S., Seong, Y. H. and Bae, K. H. : Cytotoxic saponins from the root of Dipsacus asper Wall. Arch. Pharm. Res. 28, 1053 (2005) https://doi.org/10.1007/BF02977401
  11. Halliwell, B. : Drug antioxidant effects, A basis for drug selection. Drugs 42, 569 (1991) https://doi.org/10.2165/00003495-199142040-00003
  12. Fukujawa, K. and Takaishi, Y. : Antioxidants. J. Act. Oxyg. Free Rad. 1, 55 (1990)
  13. 皆川信子 : 活性酸素か關與する代表的疾患. フアルマシア 29, 1029 (1993)
  14. Luc, G. and Fruchart, J. C. : Oxidation of lipoproteins and artherosclerosis. Am. J. Clin. Nutr. 53, 206 (1991) https://doi.org/10.1093/ajcn/53.1.206S
  15. Inkeda, N. and Fukuzume, K. : Tocopherols as antioxidants in oxidation of methyl linolate. J. Japan Oil Chem. Soc. 26, 343 (1977) https://doi.org/10.5650/jos1956.26.343
  16. Converse, C. A. and Skinmer, E. R. : Lipoprotein analysis, A practical approach. Oxford University, New York p. 113 (1992)
  17. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. : Protein measurement with the Folin-Phenol reagent. J. Biol. Chem. 193, 265 (1951)
  18. Choi, J. H., Park, Y. J., Son, H. S., Yang, K. S. and Kim, T. W. : Functional properties of modified low density lipoprotein and degradation of modified LDL by human monocyte macrophage. J. Kor. Soc. Food Nutr. 24, 362 (1995)
  19. Chiu, H. C., Jeng, J. R. and Shieh, S. M. : Increase of oxidizability of plasma low density lipoproteinfrom patients with coronary artery disease. Biochim. Biophy. Acta 12, 200 (1994)
  20. Kim, S. S., Park, R. Y., Jeon, H. J., Kwon, Y. S. and Chun, W. : Neuroprotective effects of 3,5-dicaffeoylquinic acid on hydrogen peroxide-induced cell death in SH-SY5Y cells. Phytother. Res. 19, 243 (2005) https://doi.org/10.1002/ptr.1652
  21. Ide, N., Nelson, A. B. and Lau, B. H. : Aged galic extract and its constituents inhibit $Cu^{2+}$-induced oxidative modification of low density lipoprotein. Planta Medica 63, 263 (1997) https://doi.org/10.1055/s-2006-957668
  22. Palinski, W., Rosenfeld, M. E., Yla, H. S., Gurtner, G. C., Socher, S. S., Butler, S. W., Parthasarathy, S., Carew, T. E., Steinberg, D. and Witzum J. L. : Low density lipoprotein undergoes oxidative modification in vivo. Proc. Natl, Acad. Sci. 86, 1372 (1989)
  23. Hodis, H. N., Kramsch, D. M., Avogaro, P., Bittolo-Bon, G., Cazzolato, G., Hwang, J., Peterson, H. and Sevanian, A. : Biochemical and cytotoxic characteristics of an in vivo circulating oxidized low density lipoprotein (LDL). J. Lipid Res. 35, 669 (1994)
  24. Yan, L. J., Droy-Lefaix, M. T. and Packer, L. : Ginkgo biloba extract (EGb 761) protects human low density lipoproteins against oxidative modification mediated by copper. Biochem. Biophys. Res. Commun. 212, 360 (1995) https://doi.org/10.1006/bbrc.1995.1978
  25. Jialal, I., Norkus, E. P., Cristol, L. and Grundy, S. M. : $\beta$- Carotene inhibits the oxidative modification of low-density lipoprotein. Biochem. Biophy. Acta 1086, 134 (1991) https://doi.org/10.1016/0005-2760(91)90164-D
  26. Korolainen, M. A., Goldsteins, G., Nyman, T. A., Alafuzoff, I., Koistinaho, J. and Pirttila, T. : Oxidative modification of proteins in the frontal cortex of Alzheimer's disease brain. Neurobiol. Aging. 27, 42 (2005)
  27. Zhang, Z. J., Qian, Y. H., Hu, H. T., Yang, J. and Yang, G. D. : The herbal medicine Dipsacus asper Wall extract reduces the cognitive deficits and overexpression of beta-amyloid protein induced by aluminum exposure. Life Sci. 73, 2443 (2003) https://doi.org/10.1016/S0024-3205(03)00649-0
  28. Mahato, S. B., Ganguly, A. N. and Sahu, N. P. : Steroid saponins. Phytochem. 21, 959 (1982) https://doi.org/10.1016/S0031-9422(00)82400-0
  29. Seo, S., Tori, Y. and Yoshimura, Y. : Detection of the absolute configuration of a secondary hydroxyl group in a chiral secondary alcoholusing glycosidation shifts in carbon-13 nuclear magnetic resornance spectroscopy. J. Am. Chem. Soc. 100, 3331 (1978) https://doi.org/10.1021/ja00479a014
  30. Chandel, R. S. and Rastogi, R. P. : Triterpenoidal sapogenins: 1973-1978. Phytochem. 19, 1889 (1980) https://doi.org/10.1016/0031-9422(80)83001-9
  31. Mahato, S. B., Sarkar, S. K. and Poddar, F. : Terpenoid saponins. Phytochem. 27, 3037 (1988) https://doi.org/10.1016/0031-9422(88)80001-3
  32. Higuchi, R. and Kawaski, T. : Seed saponins of Akebia quinata Decne. II. Hederagenin 3,28-O-bisglycosides. Chem. Pharm. Bull. 20, 2143 (1972) https://doi.org/10.1248/cpb.20.2143