Journal of Microbiology

  • 제44권1호
  • /
  • Pages.126-128
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  • 2006
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Removal of Contaminating TEM-la $\beta-Lactamase$ Gene from Commercial Taq DNA Polymerase

  • Song Jae Seok (Department of Biological Sciences, Myongji University) ;
  • Lee Jung Hun (Department of Biological Sciences, Myongji University, Marine Biotechnology Center, Korea Ocean Research & Development Institute) ;
  • Lee Jung-Hyun (Marine Biotechnology Center, Korea Ocean Research & Development Institute) ;
  • Jeong Byeong Chul (Department of Biological Sciences, Myongji University) ;
  • Lee Won-Keun (Department of Biological Sciences, Myongji University) ;
  • Lee Sang Hee (Department of Biological Sciences, Myongji University)
  • 발행 : 2006.02.01
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초록

This study confirms that Taq DNA polymerase could be contaminated with the $blaTEM-1_a$ gene. It also proposes two different methods that could be used to overcome DNA contamination: (i) DNase I treatment prior to PCR amplification; and (ii) the use of a highly purified Taq DNA polymerase which was devoid of detectable contamination.

키워드

참고문헌

  1. Bottger, E.C. 1990. Frequent contamination of Taq DNA polymerase with DNA. Clin. Chem. 36, 1258-1259
  2. Bradford, P.A. 2001. Extended-spectrum $\beta$-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin. Microbiol. Rev. 14, 933-951 https://doi.org/10.1128/CMR.14.4.933-951.2001
  3. Chiang, C.-S., C.-P. Liu, L.-C. Weng, N.-Y. Wang, and G.J. Liaw. 2005. Presence of$\beta$-lactamase gene TEM-1 DNA sequence in commercial Taq DNA polymerase. J. Clin. Microbiol. 43, 530-531 https://doi.org/10.1128/JCM.43.1.530-531.2005
  4. Corless, C.E., M. Guiver, R. Borrow, V. Edwards-Jones, E.B. Kaczmarski, and A. J. Fox. 2000. Contamination and sensitivity issues with a real-time universal 16S rRNA PCR. J. Clin. Microbiol. 38, 1747-1752
  5. Hughes, M.S., L.A. Beck, and R.A. Skuce. 1994. Identification and elimination of DNA sequences in Taq DNA polymerase. J. Clin. Microbiol. 32, 2007-2008
  6. Jeong, S.H., I.K. Bae, J.H. Lee, S.G. Sohn, G.H. Kang, G.J. Jeon, Y.H. Kim, B.C. Jeong, and S.H. Lee. 2004. Molecular characterization of extended-spectrum $\beta$-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli from a Korean national survey. J. Clin. Microbiol. 42, 2902-2906 https://doi.org/10.1128/JCM.42.7.2902-2906.2004
  7. Lee, S.M., S.Y. Yoo, H.-S. Kim, K.-W. Kim, Y.-J. Yoon, S.-H. Lim, H.-Y. Shin, and J.-K. Kook. 2005. Prevalence of putative periodontopathogens in subgingival dental plaques from gingivitis lesions in Korean orthodontic patients. J. Microbiol. 43, 260-265
  8. Livermore, D.M. 1995. $\beta$-lactamases in laboratory and clinical resistance. Clin. Microbiol. Rev. 8, 557-584
  9. Newsome, T., B.-J. Li, N. Zou, and S.-C. Lo. 2004. Presence of bacterial phage-like DNA sequences in commercial Taq DNA polymerase reagents. J. Clin. Microbiol. 42, 2264-2267 https://doi.org/10.1128/JCM.42.5.2264-2267.2004
  10. Paterson, D.L., W.-C. Ko, A. Von Gottberg, J.M. Casellas, L. Mulazimoglu, K.P. Klugman, R.A. Bonomo, L.B. Rice, J.G. McCormack, and V.L. Yu. 2001. Outcome of cephalosporin treatment for serious infections due to apparently susceptible organisms producing extended-spectrum $\beta$-lactamases: Implications for the clinical microbiology laboratory. J. Clin. Microbiol. 39,. 2206-2212 https://doi.org/10.1128/JCM.39.6.2206-2212.2001
  11. Rand, K.H. and H. Houck. 1990. Taq polymerase contains bacterial DNA of unknown origin. Mol. Cell. Probes 4, 445-450 https://doi.org/10.1016/0890-8508(90)90003-I
  12. Sanders, C.C. and W.E. Jr Sanders. 1992. $\beta$-lactamase in Gram-negative bacteria: global trends and clinical impact. Clin. Infect. Dis. 15, 824-839 https://doi.org/10.1093/clind/15.5.824
  13. Schmidt, T.M., B. Pace, and N.R. Pace. 1991. Detection of DNA contamination in Taq polymerase. BioTechniques 11, 176-177
  14. Song, J.S., J.H. Jeon, J.H. Lee, S.H. Jeong, B.C. Jeong, S.-J. Kim, J.-H. Lee, and S.H. Lee. 2005. Molecular characterization of TEM-type $\beta$-lactamases identified in cold-seep sediments of Edison seamount (south of Lihir Island, Papua New Guinea). J. Microbiol. 43, 172-178
  15. Sutcliffe, J.G. 1978. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc. Natl. Acad. Sci. USA 75, 3737-3741
  16. Tondeur, S., O. Agbulut, M.-L. Menot, J. Larghero, D. Paulin, P. Menasche, J.-L. Samuel, C. Chomienne, and B. Cassinat. 2004. Overcoming bacterial DNA contamination in real-time PCR and RT-PCR reactions for LacZ detection in cell therapy monitoring. Mol. Cell. Probes 18, 437-441 https://doi.org/10.1016/j.mcp.2004.08.002