Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Analysis of Mono-, Di- and Tri-glycerides by high-performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD)

HPLC/ELSD에 의한 Mono-, Di- 및 Tri-glycerides류 분석

  • Lee, Man-Ho (Department of Applied Chemistry, Kyungpook National University) ;
  • Park, Heai-Ku (Department of Chemical System Engineering, Keimyung University) ;
  • Kim, In-Whan (Department of Chemistry Education, Daegu University)
  • 이만호 (경북대학교 응용화학과) ;
  • 박희구 (계명대학교 시스템화학과) ;
  • 김인환 (대구대학교 화학교육과)
  • Received : 2006.03.13
  • Accepted : 2006.06.01
  • Published : 2006.06.27
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Abstract

Chromatographic separation of glycerol monostearate, glycerol distearate and glycerol tristearate (GMS, GDS, and GTS) has been performed by normal phase HPLC method utilizing a Zorbax silica ($250{\times}4.6mm$, $5{\mu}m$) column and hexane-hexane, IPA and ethyl acetate mixtures as the eluent within 20 min. The observed reproducibility was less than 5% RSD, Suggesting that ELSD was an effective tool for detection of the glycerol stearates of low volatility without chromophore. The detection limits were in the concentration range of 0.3~2 mg/L, and the calibration curves (the log-log plots) were linear in the range of 4~1000 mg/L (with the slopes of 1.06~1.32). The application of the analytical procedure without pretreatment demonstrated that the proposed chromatographic method would be practical for a routine analysis of commercial products.

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References

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