Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
권호 표지

Histological Study of the Minute Tubercles on Larval Skin Surface of a Korean Endemic Bitterling, Acheilognathus koreensis (Pisces, Cyprinidae), with Its Larval Growth

칼납자루, Acheilognathus koreensis (어강, 잉어목) 자어의 표피돌기에 관한 조직학적 연구

  • Kim, Chi-Hong (Inland Fisheries Ecological Research Institute, NFRDI) ;
  • Park, Jong-Young (Faculty of Biological Sciences and Institute for Biodiversity Research, Chonbuk National University) ;
  • Park, Min-Kyong (Faculty of Biological Sciences and Institute for Biodiversity Research, Chonbuk National University) ;
  • Kang, Eon-Jong (Inland Fisheries Aquaculture Research Institute) ;
  • Kim, Jong-Hwa (Inland Fisheries Ecological Research Institute, NFRDI)
  • 김치홍 (국립수산과학원 내수면생태연구소) ;
  • 박종영 (전북대학교 생물과학부.전북대학교 부설 다양성연구소) ;
  • 박민경 (전북대학교 생물과학부.전북대학교 부설 다양성연구소) ;
  • 강언종 (국립수산과학원 내수면양식연구소) ;
  • 김종화 (국립수산과학원 내수면생태연구소)
  • Received : 2006.07.09
  • Accepted : 2006.08.30
  • Published : 2006.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Morphology and distribution of the minute tubercles projected on the skin surface of larvae with its development was observed in the Korean bitterling, Acheilognathus koreensis, known as an endemic freshwater fish. The epidermis of the larvae consisted of a thin single layer, having smaller basophilic flat or round-flattened basal cells. In between the single cell layer, two or three layers were added and they consisted mainly of large epidermal cells just above basal cells. These large unicellular epidermal cells were mainly scale-shaped and rarely cone-shaped, and do not give any histochemical tests for mucosubstances. They were present in anterior region and most region of yolk sac. Whereas, vestigial epidermal cells were distributed in the body region and the caudal fin-fold region. These two kinds of epidermal cells, called minute tubercles, increased in number and height from Just to 8 days after hatching, but as the larvae develop gradually, they became to reduce. At 31days after hatching of free swimming stage and absolute absorption stage of the yolk sac, the minute tubercles did not exist on the whole skin of the larvae.

한국 고유종인 칼납자루의 자어시기 표피에서 돌출되어 나타나는 미세돌기의 형태 및 분포를 자어의 발생단계에 따라 조직학적으로 조사한 결과 자어의 표피는 소형의 호염기성이면서 편평한 기저세포로 구성된 얇은 한 층의 세포층으로 구성되어 있다. 이러한 표피층은 발생이 진행되면서 기저세포 위에 커다란 상피세포)로 구성된 2층이 형성된다. 이러한 커다란 상피세포는 단세포로서 비늘모양으로 난황낭 앞부분과 난황 부분에서 출현하여, 탄수화물테스트에 어떠한 반응을 보이지 않았다. 또한 자어의 몸통과 미병부에서는 흔적적인 상피세포가 존재한다. 이러한 두 종류의 상피세들은 미세돌기로 알려져 있다. 이러한 세포들은 부화 직후부터 부화 8일까지 수와 크기가 증가하지만 자어가 발달함에 따라 점점 감소를 반복하다 유영시기와 난황이 완전 흡수되는 시기인 부화 31일에 더 이상 존재하지 않는다.

Keywords

Acknowledgement

Supported by : NFRDI

References

  1. Aldridge, D.C. 1999. Development of European bitterling in the gills of freshwater mussels. J. Fish Biol., 54 : 138-151 https://doi.org/10.1111/j.1095-8649.1999.tb00618.x
  2. Arai, R., S.R. Jeon and T. Ueda. 2001. Rhodeus pseudosericeus sp. nov., a new bitterling from South Korea (Cyprinidae, Acheilognathinae). Ichthyol. Res., 48 : 275-282 https://doi.org/10.1007/s10228-001-8146-1
  3. Arai, R. and Y. Akai. 1988. Acheilognathus melanogaster, a senior synonym of A. moriokae, with a revision of the genera of the subfamily Acheilognathinae (Cypriniformes, Cyprinidae). Bulletin of the National Science Museum, Series A, 14 : 199-213
  4. Berra, T.M. 2001. Freshwater fish distribution. Academic press. New York, 604 pp
  5. Candolin, U. and J.D. Reynolds. 2001. Sexual signaling in the European bitterling: females learn the truth by direct inspection of the resource. Behav. Ecol., 12 : 407-411 https://doi.org/10.1093/beheco/12.4.407
  6. Fukuhara, S., Y. Nagata and W. Maekawa, 1982. Minute scaly tubercles on the yolksac of rhodeine cyprinid fishes in prolarval stages. Japan. J. Ichthyol., 29 : 232-236. (in Japanese)
  7. Heschl, A. 1989. Integration of 'innate' and 'learned' components within the IRME for mussel recognition in the European bitterling Rhodeus amarus (Bloch). Ethology, 81 : 193-208
  8. Kawamura, K. and K. Uehara. 2005. Effects of temperature on free embryonic diapause in the autumn spawning bitterling Acheilognathus rhombeus (Teleostei: Cyprinidae). J. Fish Biol., 67 : 684-695 https://doi.org/10.1111/j.0022-1112.2005.00769.x
  9. Kim I.S. and C.H. Kim. 1990. A new acheilognathine fish Acheilognathus koreensis (Pisces: Cyprinidae) from Korea. Korean J. Ichthyol., 7 : 47-52
  10. Kim, I.S., Y. Choi, C.L. Lee, Y.J. Lee, B.Y. Kim and J.H. Kim. 2005. Illustrated book of Korean fishes. Kyo-Hak Publishing Co. Ltd., 613 pp (in Korean)
  11. Kim, I.S. and J.Y. Park. 2002. Freshwater fishes of Korea. Kyo-Hak Publishing Co. Ltd., 463 pp (in Korean)
  12. Laale, H.W. 1980. The perivitelline space and egg envelopes of bony fishes; a reviews. Copeia, 1980 : 210-226 https://doi.org/10.2307/1443999
  13. Lev, R. and S.S. Spicer. 1964. Specific staining of sulphated groups with alcian blue at low pH. J. Histochem. Cytochem., 12 : 309
  14. Lillie, R.D. and J. Greco. 1947. Malt diastase and ptylin in place of saliva in the identification of glycogen. Stain Techn., 22 : 67-70
  15. Mills, S.C. and J.D. Reynolds. 2003. Operational sex ratio and alternative reproductive behaviours in the European bitterling, Rhodeus sericeus. Behav. Ecol. Sociobiol., 54 : 98-104
  16. Nakamura, M. 1969. Cyprinid fishes of Japan. Special publications of the Research Institute for Natural Resources, No. 4. Tokyo: Research Institute for Natural Resources
  17. Nelson, J.S. 1984. Fishes of the world. A Willey-Interscience Publication, New York, 523 pp
  18. Reynolds, J.D. and H.P. Guillaume. 1998. Effects of phosphate on the reproductive symbiosis between bitterling and freshwater mussels: implications for conservation. J. Appl. Ecol., 35 : 575-581 https://doi.org/10.1046/j.1365-2664.1998.3540575.x
  19. Smith, C., M. Reichard, P. Jurajda and M. Przybylski. 2004. The reproductive ecology of the European bitterling (Rhodeus sericeus). J. Zool., 262 : 107-124 https://doi.org/10.1017/S0952836903004497
  20. Steedman, H.F. 1950. Alcian blue 8G: a new stain for mucin. Quart. J. Micr. Sci., 91 : 477-479
  21. Suzuki, N. and T. Hibia. 1984. Development of eggs and larvae of two bitterlings, Rhodeus atremius and R. suigensis (Cyprinidae). Japan. J. Ichthyol., 31: 287-296
  22. Suzuki, N. and S.R. Jeon, 1987. Development of the bitterling, Acheilognathus yamatsutae (Cyprinidae), with notes on minute tubercles on the skin surface and paryngeal apparatus. Korean. J. Limnol., 20: 229-241. (in Korean with English abstract)
  23. Suzuki, N. and S.R. Jeon, 1988a. Development of the bitterling, Acheilognathus signifer (Cyprinidae), with note on minute tubercles on the skin surface. Korean J. Limnol., 21 : 165-179
  24. Suzuki, N. and S.R. Jeon, 1988b. Development of eggs, larvae and juveniles of Rhodeus ocellatus from Ansungriver, Korea (Pisecs: Cyprinidae), with notes on minute tubercles on the skin surface. Korean J. Limnol., 21 : 1-15. (in Korean with English abstract)
  25. Suzuki, N. and S.R. Jeon, 1988c. Development of the bitterling, Acheilognathus limbata (Cyprinidae) from Korea and Japan, with notes on minute tubercles on the skin surface and on the genetic implication in hybrid embryos. Korean J. Limnol., 21 : 211-229
  26. Suzuki, N. and S.R. Jeon, 1988d. Development of the bitterling, Acheilognathus suigensis (Cyprinidae) from Korea, with note on minute tubercles on the skin surface. Korean J. Limnol., 21 : 231-242. (in Korean with English abstract)
  27. Suzuki, N. and S.R. Jeon, 1989. Development of the bitterling, Acanthorhodeus asmussi (Cyprinidae) with note on minute tubercles on the skin surface. Korean J. Ichthyol., 1 : 73-82 (in Korean with English abstract)
  28. Suzuki, N. and S.R. Jeon, 1990. Development of the bitterling, Acanthorhodeus geacilis (Cyprinidae), with note on minute tubercles on the skin surface. Korean. J. Ichthyol., 2 : 169-181