Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Identification of Tricholoma matsutake in a Pine Root by ITS Region Amplification and RAPD Analysis with Different Mycorrhiza

ITS 영역 증폭에 의한 소나무 송이균 뿌리 감염 확인 및 RAPD에 의한 타 균근과의 비교

  • Kim, Myungkil (Div. Wood Chemistry & Microbiology, Korea Forest Research Institute) ;
  • Ryu, Sun-Hwa (Div. Wood Chemistry & Microbiology, Korea Forest Research Institute) ;
  • Bak, Wonchull (Div. Wood Chemistry & Microbiology, Korea Forest Research Institute) ;
  • Park, Hyun (Div. Wood Chemistry & Microbiology, Korea Forest Research Institute) ;
  • Ka, Kang-Hyeon (Div. Wood Chemistry & Microbiology, Korea Forest Research Institute) ;
  • Sohn, Hee-Kyung (Div. Wood Chemistry & Microbiology, Korea Forest Research Institute)
  • 김명길 (국립산림과학원 화학미생물과) ;
  • 유선화 (국립산림과학원 화학미생물과) ;
  • 박원철 (국립산림과학원 화학미생물과) ;
  • 박현 (국립산림과학원 화학미생물과) ;
  • 가강현 (국립산림과학원 화학미생물과) ;
  • 손희경 (국립산림과학원 화학미생물과)
  • Received : 2006.08.25
  • Accepted : 2006.10.23
  • Published : 2006.11.25
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Abstract

A simple method for identifying of ectomycorrhizal fungi was presented, using the polymerase chain reaction (PCR) to amplify the ITS (Internal transcribed spacer) regions of the nuclear ribosormal repeat. The sequences analyzed 6 species, Pisolithus tinctorius, Chroogomphus rutilus, Leucogyrophana pinastri, Suillus granulatus, Lactarius laeticolorus, and Suillus bovinus at hongreung forest, and analysed 10 species, Craterellus lutescens, Thelephoroid mycorrhizal, Lactarius quieticolor, Tricholoma matsutake, Lactarius chrysorrheus, Sarcodon aspratus, Russula versicolor, Suillus luteus, Tricholoma terreum, and Amanita vaginata at hongcheon forest. Finally, the amplification by PCR with ITS1-ITS4 primers offers good results over classical identification for ectomycorrhizal fungi species.

대량 배양된 송이 균사를 배양병 안에 있는 소나무에 접종한 접종묘를 실시하였는데, 접종묘 내 소나무 뿌리내로 송이 균사가 침투하여 감염이 되었는지를 판별하는 방법으로 ITS (Internal transcribed spacer) 영역에서 특이 primer set인 ITS1-ITS4 (TCCGTAGGTGAACCTGCGG/TCCTCCGCTTATGATATGC)를 선택하여 정하고, 그를 바탕으로 홍천 시험지(강원도 홍천군 동면 노천리)와 홍릉수목원(서울시 동대문구 청량리 2동) 내에서 수집한 다른 균근과 비교하고자 RAPD (Rapid Amplified Polymorphic DNA) 기법으로 각 6종, 10 종의 균근을 동정하였다.

Keywords

References

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