Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Optimum Condition of HPLC by HCI Program

HCI 프로그램을 이용한 HPLC의 최적화 조건

  • Jin, Chun Hua (Department of Chemical Engineering, Inha University) ;
  • Lee, Ju Weon (Department of Chemical Engineering, Inha University) ;
  • Row, Kyung Ho (Department of Chemical Engineering, Inha University)
  • Received : 2006.06.30
  • Accepted : 2006.08.30
  • Published : 2006.12.31
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Abstract

Recently, liquid chromatography (LC) has been used more frequently to separate drugs and natural substances. Especially, to selection of the solutes from the products, the operation condition of analytical chromatography should be necessarily determined. So accurate computer modeling and simulation of chromatographic performances has become a necessary part of the development and design of processes. High-Purity Separation Lab. Inha University developed the resulting HCI software for the purpose of the optimization of chromatographic performances. The HCI program was utilized to find the optimum operating condition more accurately and rapidly, reducing the number of many possible experiments. The elution profiles were calculated by the plate theory based on the three retention mechanism of capacity factor.

최근 액체크로마토그래피는 의학상이나 천연물질 분리에 많이 사용되고 있다. 특히 혼합물에서 유용성분을 분취하기 위해서는 우선 분석용 크로마토그래피의 조업조건이 결정되어야 한다. 때문에 정확한 수학적 모델링과 시뮬레이션은 크로마토그래피의 발전과 디자인 과정에서 필요한 부분이다. 인하대학교 고 순도분리연구실에서는 크로마토그래피의 최적화를 목적으로 HCI 프로그램을 개발하였다. 이 과정에서 최소한의 실험으로 보다 정확하고 빠른 최적 조업조건을 HCI 프로그램을 이용하여 구할 수 있다. 즉 이론단수를 이용하여 체류인자를 예측하는 세가지 모델식으로부터 용출곡선을 계산할 수 있으며 분리도와 분리시간을 기준으로 최적 이동상의 조건을 계산할 수 있다.

Keywords

References

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