Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Comparative Analysis of Growth-Phase-Dependent Gene Expression in Virulent and Avirulent Streptococcus pneumoniae Using a High-Density DNA Microarray

  • Ko, Kwan Soo (Asian-Pacific Research Foundation for Infectious Diseases (ARFID)) ;
  • Park, Sulhee (Asian-Pacific Research Foundation for Infectious Diseases (ARFID)) ;
  • Oh, Won Sup (Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Suh, Ji-Yoeun (Asian-Pacific Research Foundation for Infectious Diseases (ARFID)) ;
  • Oh, TaeJeong (GenomicTree, Inc.) ;
  • Ahn, Sungwhan (GenomicTree, Inc.) ;
  • Chun, Jongsik (School of Life Sciences, Seoul National University) ;
  • Song, Jae-Hoon (Asian-Pacific Research Foundation for Infectious Diseases (ARFID))
  • Received : 2005.08.23
  • Accepted : 2005.11.25
  • Published : 2006.02.28
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Abstract

The global pattern of growth-dependent gene expression in Streptococcus pneumoniae strains was evaluated using a high-density DNA microarray. Total RNAs obtained from an avirulent S. pneumoniae strain R6 and a virulent strain AMC96-6 were used to compare the expression patterns at seven time points (2.5, 3.5, 4.5, 5.5, 6.0, 6.5, and 8.0 h). The expression profile of strain R6 changed between log and stationary growth (the Log-Stat switch). There were clear differences between the growth-dependent gene expression profiles of the virulent and avirulent pneumococcal strains in 367 of 1,112 genes. Transcripts of genes associated with bacterial competence and capsular polysaccharide formation, as well as clpP and cbpA, were higher in the virulent strain. Our data suggest that late log or early stationary phase may be the most virulent phase of S. pneumoniae.

Keywords

Acknowledgement

Supported by : Ministry of Commerce, Industry and Energy

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