Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Effect of Light-emitting Diodes (LEDs) and Ventilation on the in vitro Shoot Growth of Eucalyptus pellita

Eucalyptus pellita의 기내(器內) 줄기생장에 미치는 LEDs (Light-emitting diodes) 및 환기처리(換氣處理) 효과

  • Kim, Ji-Ah (Biotechnology Div., Korea Forest Research Institute) ;
  • Moon, Heung Kyu (Biotechnology Div., Korea Forest Research Institute)
  • 김지아 (국립산림과학원 생물공학과) ;
  • 문흥규 (국립산림과학원 생물공학과)
  • Received : 2006.08.30
  • Accepted : 2006.10.09
  • Published : 2006.12.30
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Abstract

Various light sources including LEDs (Light emitting diodes) affecting on shoot growth was examined using in vitro shoots of E. pellita. Generally, it appeared that ventilation treatment was the most important factor affecting on normal shoot growth, irrespective of irradiation sources. Ventilation resulted in better performance of the cultures under 100% blue LED radiation. These include better shoot growth, more number of leaves, more number of internodes, more number of axillary buds, and heavier dry matters. The highest total chlorophyll content was obtained under both cool-white fluorescent lamps and R5B5 (50% red LED + 50% blue LED). The value was $24.5{\mu}g/g$ and $20.1{\mu}g/g$, respectively. In addition, ventilation resulted in higher carotenoid content in all irradiation sources except 100% red LED radiation. In conclusion, shoot growth of E. pellita could be reached maximum by ventilation under R5B5 (50% red LED + 50% blue LED).

Eucalyptus pellita 기내줄기를 재료로 광질에 따른 생육 특성을 조사하였다. 전반적으로 줄기의 생장은 광질보다는 환기처리가 더 주요한 요인으로 나타났다. 특히 환기처리 한 청색광(blue LED) 하에서 묘고, 잎수, 절간수 및 액아수가 양호하였고, 잎에 캘러스가 형성되지 않았다. 식물체의 충실정도를 나타내는 건물율 역시 환기처리 하에서 높게 나타났으며, 청색광 하에서 가장 높은 건물율을 나타냈다. 광질별 총엽록소 함량은 형광등과 혼합광 R5B5(50% red LED + 50% blue LED)에서 $24.5{\mu}g/g$ 및 $20.1{\mu}g/g$으로 가장 높게 나타났다. 광합성에 관여하는 카로티노이드 함량 역시 적색광(red LED)을 제외한 모든 광질에서 환기 처리 시 양호하게 나타났다. 결론적으로 E. pellita의 기내 줄기생장은 환기처리가 중요하고, 총 카로티노이드 함량을 기준으로 볼 때 혼합광 R5B5에서 건전한 줄기생장이 가능한 것으로 나타났다.

Keywords

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