Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
권호 표지

Survey of Genes Responsive to Long-Term Heat Stress Using a cDNA Microarray Analysis in Mud Loach (Misgurnus mizolepis) Liver

장기 고온 스트레스에 대한 미꾸라지(Misgurnus mizolepis) 간 조직 내 유전자 발현 반응의 cDNA microarray 분석

  • Cho, Young Sun (Department of Aquaculture, Pukyong National University) ;
  • Lee, Sang Yoon (Department of Aquaculture, Pukyong National University) ;
  • Noh, Choong Hwan (Marine Resources and Research Department, Korea Ocean Research and Development Institute) ;
  • Nam, Yoon Kwon (Institute of Marine Living Modified Organisms (iMLMO) Pukyong National University) ;
  • Kim, Dong Soo (Institute of Marine Living Modified Organisms (iMLMO) Pukyong National University)
  • 조영선 (부경대학교 양식학과) ;
  • 이상윤 (부경대학교 양식학과) ;
  • 노충환 (한국해양연구원 생물자원본부) ;
  • 남윤권 (부경대학교 해양수산형질전환생물연구소) ;
  • 김동수 (부경대학교 해양수산형질전환생물연구소)
  • Received : 2006.03.25
  • Accepted : 2006.05.15
  • Published : 2006.06.30
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Abstract

Gene transcripts potentially responsive to the heat stress were surveyed by cDNA microarray analysis in mud loach (Misgurnus mizolepis). Transcriptional profiles of hepatic tissue in the fish exposed to either $23^{\circ}C$ or $32^{\circ}C$ for 4 weeks were compared each other by 3 replicated hybridization assays using 1,124 unigene clones selected from mud loach liver expressed sequence tags (ESTs). A total of 93 clones showed the substantially increased mRNA levels (>2-fold) in $32^{\circ}C$-exposed group when compared in $23^{\circ}C$control group. It includes various enzymes and proteins involved in energy pathway, protease/protein metabolisms, immune/antioxidant functions, cytoskeleton/cell structure, transport and/or signal transduction. Maximum level of increase was up to 15-fold relative to $23^{\circ}C$ treatment. Heat exposure also resulted in the significant decrease (less than 50% relative to $23^{\circ}C$-exposed fish) of the transcriptional activities in 85 genes. Besides the above categories, yolk protein (vitellogenin) and ribosomal proteins were notably down regulated in the fish exposed to heat stress. A number of novel gene transcripts were also detected in both up-regulated and down-regulated groups.

우리나라 주요 담수 어종인 미꾸라지(Misgurnus mizolepis)를 실험 모델로 이용하여 실험적으로 설정한 고온 노출($32^{\circ}C$)에 특이적으로 반응하는 유전자들을 cDNA microarray 분석을 통해 탐색하였다. 미꾸라지 간조직 expressed sequence tag (EST) 데이터베이스 분석을 통해 1,124개의 unigene들을 선발하여 제작한 cDNA microarray을 이용하여 $23^{\circ}C$ 및 $32^{\circ}C$에 4주간 노출된 실험어의 간(liver)조직의 전사 발현 양상을 3반복 분석하였다. 다양한 유전자군이 $32^{\circ}C$ 고온 노출에 전사 발현의 증감 또는 감소 양상을 보였으며 $23^{\circ}C$에 비해 $32^{\circ}C$군에서 2배 이상의 발현 증가를 보인 클론들은 총 93종류로서 에너지 대사, 단백질 대사, 면역/항산화 기능, 세포골격 및 구조, 물질수송 및 세포 신호전달등에 관여하는 단백질들을 암호화하는 유전자들이었고 최대 15배 이상의 전사발현이 관찰되었다. 반면 고온 노출군에서 유의적인 발현 감소(50% 이하)를 보인 유전자들(n=85) 역시 탐색되어 상기 단백질 분류군외에 vitellogenin 전구체들 및 리보좀 단백질류에서 특이적인 전사활성의 저하가 관찰되었고, vitellogenin 유전자에서 가장 많은 mRNA 수준의 감소가 관찰되었다.

Keywords

Acknowledgement

Supported by : 한국학술진흥재단

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