Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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The Effect of Various Methods of Cross-linking in Type I Collagen Scaffold on Cartilage Regeneration

I형 콜라겐지지체의 다양한 가교처리 방법이 연골막성 연골재생에 미치는 영향

  • Son, Dae Gu (Department of Plastic and Reconstructive Surgery, Keimyung University, Dongsan Medical Center) ;
  • Lim, Joong Jae (Duo Plastic Surgery Clinic) ;
  • Sohn, Kyounghee (Department of Plastic and Reconstructive Surgery, Keimyung University, Dongsan Medical Center) ;
  • Yang, Eun Kyung (Bioland)
  • 손대구 (계명대학교 의과대학 성형외과학교실) ;
  • 임중재 (듀오 성형외과) ;
  • 손경희 (계명대학교 의과대학 성형외과학교실) ;
  • 양은경 (바이오랜드)
  • Received : 2006.04.27
  • Published : 2006.11.10
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Abstract

Purpose: Collagen is the principal structural biomolecule in cartilage extracellular matrix, which makes it a logical target for cartilage engineering. In this study, porous type I collagen scaffolds were cross-linked using dehydrothermal(DHT) treatment and/or 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide(EDC), in the presence and absence of chondroitin-6-sulfate(CS) for cartilage regeneration. Methods: Cartilage defects were created in the proximal part of the ear of New Zealand rabbits. Four types of scaffolds(n=4) were inserted. The types included DHT cross-linked(Group 1), DHT and EDC cross- linked(Group 2), CS added DHT cross-linked(Group 3), and CS added DHT and EDC cross-linked(Group 4). Histomorphometric analysis and cartilage-specific gene expression of the reconstructed tissues were evaluated respectively 4, 8, and 12 weeks after implantation. Results: The largest quantity of regenerated cartilage was found in DHT cross-linked groups 1 and 3 in the 8th week and then decreased in the 12th week, while calcification increased. Calcification was observed from the 8th week and the area increased in the 12th week. Group 4 was treated with EDC cross-linking and CS, and the matrix did not degrade in the 12th week. Cartilage-specific type II collagen mRNA expression increased with time in all groups. Conclusion: CS did not increase chondrogenesis in all groups. EDC cross-linking may prevent chondrocyte infiltration from the perichondrium into the collagen scaffold.

Keywords

References

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