식물분류학회지 (Korean Journal of Plant Taxonomy)

  • 제36권4호
  • /
  • Pages.263-277
  • /
  • 2006
  • /
  • 1225-8318(pISSN)
  • /
  • 2466-1546(eISSN)
한국식물분류학회 (The Korean Society of Plant Taxonomists)
권호 표지

태백바람꽃(Anemone pendulisepala, Ranunculaceae)의 분자계통학적 검토

Molecular Phylogenetic Study of Anemone pendulisepala (Ranunculaceae)

  • 이창숙 (이화여자대학교 분자생명과학부) ;
  • 이남숙 (이화여자대학교 분자생명과학부) ;
  • 여성희 (이화여자대학교 과학교육과)
  • 투고 : 2006.11.10
  • 심사 : 2006.11.29
  • 발행 : 2006.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

태백바람꽃(Anemone pendulisepala)은 태백산에서 처음 보고된 후, 백두산에서도 발견된 적이 있으며, 회리바람꽃(A. reflexa), 들바람꽃(A. amurensis) 및 꿩의바람꽃(A. raddeana)과 혼생하여 분포하고 있다. 태백바람꽃은 총포엽의 중앙열편의 모양, 총포엽병의 길이, 꽃받침의 정단부, 줄기 내 중심주의 모양에 있어서 이들 세 분류군과 구별된다. 본 연구는 과거 제기되었던 태백바람꽃의 잡종 여부를 확인하고 분류학적 실체를 판단하기 위하여 형태적으로 유사한 회리바람꽃, 들바람꽃과 꿩의바람꽃과 함께 DNA 염기서열(ITS, psba-trnH, rps16, trnLF)을 분석하였다. 분석결과 태백바람꽃은 핵 DNA인 ITS구간에서 회리바람꽃과 동일한 염기서열을 가지며, 들바람꽃, 꿩의바람꽃 순으로 유집되었다. 태백바람꽃은 엽록체 DNA의 rps16구간에서 4개의 염기의 삽입, trnLF구간에서 2개 염기의 차이 및 6개 염기의 삽입에 의해 근연종들로부터 구분되었다. 또한 태백바람꽃은 형태적 특징에 의해 양친종으로 추정되었던 분류군들과 공유하는 염기서열이 없었고, 유전자다형성도 나타내지 않았다. 따라서 태백바람꽃은 독립된 종으로 처리되는 것이 타당하며, 유사종간의 교배종은 아닌 것으로 추정되었다.

Anemone pendulisepala, recently described as a new species, is distributed in sympatry with A. reflexa, A. amurensis, and A. raddeana at Mt. taebeark and Mt. Baekdu. Anemone pendulisepala was previously proposed to be a hybrid species between A. reflexa and A. amurensis becaue it displavs overlapping features with them in involucre shape, petiole length, sepal apex and xylem shape, To verify the taxonomic status and to examine the hybridity of A. pendulisepala, sequences of ITS region of nuclear ribosomal DNA and the psba-trnH, rps16 and trnLF region of cpDNA from 36 accessions of 5 taxa including outgroup were analyzed. In maximum parsimony tree based on ITS sequences, A. pendulisepala had the same sequences of A. reflexa and was clustered with monophyletic A. amurensis, and then A. raddeana. Anemone pendulisepala was distinguished from the other taxa by having four base insertion in rps16 region, two species-specific bases and insertion in trnLF region. In the phylogenetic trees of combined cpDNA, A. pendulisepala showed monophyly with the bootstrap 100%. Anemone pendulisepala exhibited no polymorphism and shared no sequences with putative parental or related taxa examined in this study. Molecular data suggest that A. pendulisepala should be a distinct species, and no evidence of the hybridization was detectcd.

키워드

과제정보

연구 과제 주관 기관 : 환경부

참고문헌

  1. Baldwin, B. G,, M. J. Sanderson, J. M. Porter, M. F. Wojciechowski, C. S. Camoell, and M. J. Donoghue. 1995. The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Ann. Missouri Bot. Gard. 82: 247-277 https://doi.org/10.2307/2399880
  2. Compton, J. A. and A. Culham. 2002. Phylogeny and circumscription of tribe Actaeae Ranunculaceae. Sys, Bot. 27: 502-511
  3. Cronn, R. and J. F. Wendel. 2003. Cryptic trysts, genomic mergers, and plant speciation. New Phytologist 161: 133-142 https://doi.org/10.1111/j.1469-8137.2004.00947.x
  4. Corriveau, J. L. and A. W. Coleman. 1988. Rapid screening method to detect potential biparental inheritance of plastid DNA and results for over 200 angiosperm species. Amer. J. Bot. 75: 1443-1458 https://doi.org/10.2307/2444695
  5. Chung, T. H. 1956. Korean Flora II. Shinzisa, Seoul. Pp. 1009-1010. [In Korean]
  6. Downie, S. R. and D. S. Katz-Downie. 1996. A molecular phylogeny of Apiaceae subfamily Apioideae evidence from nuclear ribosomal DNA internal transcribed spacer sequences. Amer. J. Bot. 83: 234-251 https://doi.org/10.2307/2445943
  7. Downie, S. R., D. S. Katz-Downie. and K. Spalik, 2000. A phylogeny of Apiaceae tribe Scandiceae. evidence from nuclear ribosomal DNA internal transcribed spacer sequences. Arner. J. Bot. 87: 76-95 https://doi.org/10.2307/2656687
  8. Farris, J. S., M. Kallersjo, A. G. Kluge and C. Bult. 1995. Testing significance of incongruence, Cladistics 10: 315-319 https://doi.org/10.1111/j.1096-0031.1994.tb00181.x
  9. Felsenstein, J. 1985. Confidence lirnits on phylogenies: An approach using the bootstrap. Evolution 39: 783-791 https://doi.org/10.2307/2408678
  10. Fuse, S., N. S. Lee and M. N. Tamura. 2004. Biosystematic studies of the genus Heloniopsis (Melanthiaceae) II. Two new species from Korea based on morphological and molecular evidence. Taxon 53: 949-958 https://doi.org/10.2307/4135562
  11. Gibson, T., D. Higgins and J. Thompson. 1994. Clustal X Program. EIvIBL, Heidelberg, Germany
  12. Hoot, S. B., A. A. Reznick, and J. D. Palmer. 1994. Phylogenetic Relationships in Anemone (Ranunculaceae) based on Morphology and chloroplast DNA. Syst. Bot. 19: 169-200 https://doi.org/10.2307/2419720
  13. Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitution through comparative studies of nucleotide sequences. J. Mol. Evol.: 11-120
  14. Lee, C. S., N. S. Lee and S. H. Yeau. 2004. Molecular phylogenetic relationships and speciation of Ranunculus cantoniensis (Ranunculaceae), Korean J. PI. Taxon. 34: 335-358 (in Korean) https://doi.org/10.11110/kjpt.2004.34.4.335
  15. Lee, C. S., N. S. Lee and S. H. Yeau. 2006. Taxonomic status of Goodyera rosulacea Y Lee: Molecular evidence based on ITS and tmL sequences. Korean J. PI. Taxon. 36: 189-207 (in Korean) https://doi.org/10.11110/kjpt.2006.36.3.189
  16. Lee, C. S., and S. R. Downie. 2006. Phylogenetic relationships within Cicuta (Apiaceae tribe Oenantheae) inferred from nuclear rDNA ITS and cpDNA sequence data. Can. J. Bot. 83: 453-468
  17. Lee, T B. 1980. Illustrated flora of Korea. Hyang Mun-Sa, Seoul (in Korean)
  18. Lee, W. T 1996. Coloured Standard Illustrations of Korean Plants. Academy Publishing, Seoul (in Korean)
  19. Lee, Y N. 2003. I. A new taxon of Ranunculaceae. Bulletin of Korean Plant Research. 3: 2-4 (in Korean)
  20. Lee, Y N. 2006. Flora of Korea. Gyohaksa, Seoul (in Korean)
  21. Nakai, T 1911. Flora Koreana II. J. ColI. Sci. Imp. Univ. 31: 226-227
  22. Ohwi, J. 1965. Flora of Japan. Shibundo, Tokyo (in Japanese)
  23. Oxelman, B., M. Liden and D. Berglund. 1997. Chloroplast rps16 intron phylogeny of the tribe Sileneae (Caryophyllaceae), PI. Syst. Evol. 206: 393-410 https://doi.org/10.1007/BF00987959
  24. Qiu, Y-L., C. R. Parks and M. W. Chase. 1995. Molecular divergence in the Eastern Asia-Eastern North America disjunct section Rytidospermum of Magnolia (Magnoliaceae). Amer. J. Bot. 82: 1589-1598 https://doi.org/10.2307/2446188
  25. Rieseberg, L.H. 1997. Hybrid origins of plant species. Ann. Rev. Eco. Syst. 28: 359-389 https://doi.org/10.1146/annurev.ecolsys.28.1.359
  26. Saitou, N. and M. Nei. 1987. The Neighbour-joining method: A new method for reconstructing phylogenetic trees. Molec. Biol. Evol. 4: 406-425
  27. Sang, T, D. J. Crawford and R. F. Stuessy. 1997. Chloroplast DNA phylogeny, reticulate evolution and biogeography of Paeonia (Paeoniaceae). Amer. J. Bot. 84: 1120-1136 https://doi.org/10.2307/2446155
  28. Schuettpelz. E., S. B. Hoot, R. Samuel, and F. Ehrendorfer. 2002. Multiple origins of Southern Hemisphere Anemone (Ranunculaceae) based on plastid and nuclear sequence data. Plant Syst. Evol. 231: 143-151 https://doi.org/10.1007/s006060200016
  29. Sim, J.-K, H.-D. Park and S.-J. Park. 2002. Phylogenetic study of Korean Iris (Iridaceae) based on nrDNA ITS sequences. Korean J. PI. Taxon. 32: 33-53 https://doi.org/10.11110/kjpt.2002.32.1.033
  30. Shin, K-S., Y. K Shin, J.-W. Kim and K-H. Tae. 2003. Phylogeny of the genus Goodyera (Orchidaceae: Cranichideae) in Korea based on nuclear ribosomal DNA ITS region sequences. J. Plant BioI. 45: 182-187
  31. Simmons, M. P. and H. Ochoterena. 2000. Gaps as characters in sequence-based phylogenetic analyses. Syst. Bot. 49: 369-381
  32. Swofford, D. L. 2002. PAUP*, Phylogenetic analysis using parsimony (*and other methods), Version 4,0b10, Sinauer Associates, Sunderland, Massachusetts
  33. Taberlet, P., L. Gielly, G. Pautou and J. Bouvet. 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA PI. Molec. BioI. 17: 1105-1109 https://doi.org/10.1007/BF00037152
  34. Tsai, C. 2003. Molecular phylogeny, biogeography, and evolutionary trends of the genus Phalaenopsis (Orchidaceae). Ph. D. thesis, Univ. of National Sun Yat-sen, Kaohsiung, Taiwan, Pp. 224
  35. Wallander, E. and V. A Albert. 2000. Phylogeny and classification of Oleaceae based on rps16 and tmLF sequence data. Amer. J. Bot. 87: 1827 - 1841 https://doi.org/10.2307/2656836
  36. White, T. J, T. Bruns, S. Lee, and J Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp. 315-322. In PCR Protocols: A guide to methods and applications. Innis, M. A, D. H. Gelfand, J J Sninsky and T. J White (eds.), Academic Press, San Diego
  37. Yeau, S. H. C. S. Lee and N. S. Lee, 2004, A taxonomic review of section Acris of Ranunculus L based on ITS sequences. Korean J. PI. Taxon, 34: 173-190 (in Korean) https://doi.org/10.11110/kjpt.2004.34.2.173
  38. Yoo, K., S. Jang and W. Lee, 2005. Phylogeny of Korean Viola based on ITS sequences. Korean J. PI. Taxon. 35: 7-23 (in Korean) https://doi.org/10.11110/kjpt.2005.35.1.007