IMMUNE NETWORK

  • 제6권1호
  • /
  • Pages.1-5
  • /
  • 2006
  • /
  • 1598-2629(pISSN)
  • /
  • 2092-6685(eISSN)
대한면역학회 (The Korean Association of Immunobiologists)
권호 표지

Differences in Their Proliferation and Differentiation between B-1 and B-2 Cell

  • Yeo, Seung-Geun (Department of Otolaryngology, College of Medicine, Kyung Hee University) ;
  • Cha, Chang-Il (Department of Otolaryngology, College of Medicine, Kyung Hee University) ;
  • Park, Dong-Choon (Department of Obstetric and Gynecology, St. Vincent's Hospital, The Catholic University of Korea)
  • 발행 : 2006.03.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Background: B cell subset has been divided into B-1 cells and B-2 cells. B-1 cells are found most prominently in the peritoneal cavity, as well as constituting a small pro portion of splenic B cells and they are larger and less dense than B-2 cells in morphology. This study was designed to compare the differences in their proliferation and differentiation between B-1 and B-2 cell. Methods: We obtained sorted B-1 cells from peritoneal fluid and B-2 cells from spleens of mice. Secreted IgM was measured by enzyme-linked immunosorbent assay. Entering of S phase in response to LPS-stimuli was measured by proliferative assay. Cell cycle analysis by propidium iodide was performed. p21 expression was assessed by real time PCR. Results: Cell proliferation and cell cycle progression in B-1 and B-2 cells, which did not occur in the absence of LPS, required LPS stimulation. After LPS stimulation, B-1 and B-2 cells were shifted to Sand G2/M phases. p21 expression by resting B-1 cells was higher than that of resting B-2 cells. Conclusion: B-1 cells differ from conventional B-2 cells in proliferation, differentiation and cell cycle.

키워드

참고문헌

  1. Rothstein TL: Cutting Edge: commentary. Two B-1 or not to be one. J of Immunology 168;4257-4261, 2002 https://doi.org/10.4049/jimmunol.168.9.4257
  2. Hamilton AM, Lehuen A, Kearney JF: Immunofluorescence analysis of B-1 cell ontogeny in the mouse. Int Immunol 6;355-361, 1994 https://doi.org/10.1093/intimm/6.3.355
  3. Morris DL, Rothstein TL: Decreased surface IgM receptor mediated activation of phospholipase C gamma 2 in B-1 lymphocytes. Int Immunol 6;1011-1016, 1994 https://doi.org/10.1093/intimm/6.7.1011
  4. Bikah G, Carey J, Ciallella JR, Tarakhovsky A, Bondada S: CD5-mediated negative regulation of antigen receptor-induced growth signals in B-1 B cells. Science 274;1906-1909, 1996 https://doi.org/10.1126/science.274.5294.1906
  5. Sen G, Bikah K, Venkataraman C, Bondada S: Negative regulation of antigen receptor-mediated signaling by constitutive association of CD5 with the SHP-1 protein tyrosine phosphatase in B-1 B cells. Eur J Immunol 29;3319-3328, 1999 https://doi.org/10.1002/(SICI)1521-4141(199910)29:10<3319::AID-IMMU3319>3.0.CO;2-9
  6. Rothstein TL, Kolber DL: Peritoneal B cells respond to phorbol esters in the absence of co-mitogen. J Immunol 140; 2880-2885, 1988
  7. Tanguay DA, Colarusso TP, Pavlovic S, Cirigoyen M, Howard RG, Bartek J, Chiles TC, Rothstein TL: Early induction of cyclin D2 expression in phorbol ester-responsive B-1 lymphocytes. J Exp Med 189;1685-1690, 1999 https://doi.org/10.1084/jem.189.11.1685
  8. Missero C, Di Cunto F, Kiyokawa H, Koff A, Dotto GP. The absence of p21Cip1/WAF1 alters keratinocyte growth and differentiation and promotes ras-tumor progression. Genes Dev 10;3065-3075, 1996 https://doi.org/10.1101/gad.10.23.3065
  9. Fotedar R, Brickner H, Saadatmandi N, Rousselle T, Diederich L , Munshi A: Effect of p21 (Waf1/Cip1) transgene on radiation induced apoptosis in T cells. Oncogene 18;3652- 3658, 1999 https://doi.org/10.1038/sj.onc.1202693
  10. Boulaire J, Fotedar A, Fotedar R: The functions of the cdkcyclin kinase inhibitor p21WAF1. Patho Biol 48;190-202, 2000
  11. Yother J, Forman C, Gray BM, Briles DE: Protection of mice from infection with streptococcus pneumoniae by anti-phosphocholine antibody. Infec Immun 36;184-188, 1982
  12. Angelin-Duclos C, Cattoretti G, Lin KI, Calame K: Commitment of B lymphocytes to a plasma cell fate is associated with Blimp-1 expression in vivo. J Immunol 165;5462-5471, 2000 https://doi.org/10.4049/jimmunol.165.10.5462
  13. Lin KI, Tunyaplin C, Calame K: Transcriptional regulatory cascades controlling plasma cell differentiation. Immunolo Rev 194;19-28, 2003 https://doi.org/10.1034/j.1600-065X.2003.00040.x
  14. Calame KL, Lin KI, Tunyaplin C: Regulatory mechanisms that determine the development and function of plasma cells. Annu Rev Immunol 21;205-230, 2003 https://doi.org/10.1146/annurev.immunol.21.120601.141138
  15. Tumang JR, Frances R, Yeo SG, Rothstein TL: Cutting edge: Spontaneously Ig-secreting B-1 cells violate the accepted paradigm for expression of differentiation-Associated Transcription Factors. J Immunol 174;3173-3177, 2005 https://doi.org/10.4049/jimmunol.174.6.3173
  16. Huisman MA, De Heer ED, Grote JJ: Cholesteatoma epithelium is characterized by increased expression of Ki-67, p53 and p21, with minimal apoptosis. Acta Otolaryngol 123;377-382, 2003 https://doi.org/10.1080/00016480310001376
  17. Lalmanach-Girard AC, Chiles TC, Parker DC, Rothstein TL: T cell-dependent induction of NF-$\kappa$B in B cells. J Exp Med 177;1215-1219, 993 https://doi.org/10.1084/jem.177.4.1215
  18. Ferlin WG, Severinson E, Strom L, Heath AW, Coffman RL, Ferrick DA, Howard MC: CD40 signaling induces interleukin- 4 independent IgE switching in vivo. Eur J Immunol 26;2911-2915, 1996 https://doi.org/10.1002/eji.1830261216
  19. Davey EJ, Thyberg J, Conrad D, Severinson E: Regulation of cell morphology in B ymphocytes by interleukin 4. Evidence for induced cytoskeletal changes. J Immunol 160;5366- 5373, 1998
  20. Kawahara T, Ohdan H, Zhao G, Yang YG, Sykes M. Peritoneal cavity B cells are precursors of splenic IgM natural antibody- producing cells. J Immunol 171;5406-5414, 2003 https://doi.org/10.4049/jimmunol.171.10.5406
  21. Zezula J, Casaccia-Bonnefil P, Ezhevsky SA, Osterhout DJ, Levine JM, Dowdy SF: p21cipl is required for the differentiation of oligodendrocytes independently of cell cycle withdrawal. EMBO Reports 2;27-34, 2001 https://doi.org/10.1093/embo-reports/kve008
  22. Tourigny MR, Ursini-Siegel J, Lee H, Toellner KM, Cunningham AF, Franklin DS, Ely S: CDK inhibitor p18 (INK4c) is required for the generation of functional plasma cells. Immunity 17;179-189, 2002 https://doi.org/10.1016/S1074-7613(02)00364-3
  23. Lawson BR, Baccala R, Song J, Croft M, Kono MH, Theofilopoulos AN: Deficiency of the cyclin kinase inhibitor p21 (WAF-1/CIP-1) promotes apoptosis of activated/memory T cells and inhibits spontaneous systemic autoimmunity. J of Exp Med 199;547-7, 2004 https://doi.org/10.1084/jem.20031685
  24. Missero C, Di Cunto, Kiyokawa H, Koff A, Dotto GP: The absence of p21Cip1/WAF1 alters keratinocyte growth and differentiation and promotes ras-tumor progression. Genes Dec 1;3065-75, 1996
  25. Gartel AL, Tyner AL: The role of the cyclin-dependent kinase inhibitor p21 in Apoptosis. Mol Cancer Ther 1;639-649, 2002
  26. Piatelli MJ, Tanguay D, Rothstein TL, Chiles TC: Cell cycle control mechanisms in B-1 and B-2 lymphoid subsets. Immunol Res 27;31-52, 2003 https://doi.org/10.1385/IR:27:1:31
  27. Tanguay DA, Colarusso TP, Doughty C, Pavlovic-Ewers S, Rothstein TL, Chiles TC: Cutting edge: differential signaling requirements for activation of assembled cyclin D3-cdk4 complexes in B-1 and B-2 lymphocyte subsets. J Immunol 166;4273-4277, 2001 https://doi.org/10.4049/jimmunol.166.7.4273