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The Korean Association of Immunobiologists (대한면역학회)
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Effect of Dexamethasone on the Surface Expression of Marker Molecules and Differentiation of Murine B Cells

덱사메타손이 생쥐 B세포의 세포 표면 인식자와 분화에 미치는 영향

  • Yeo, Seung-Geun (Department of Otorhinolaryngology, Head and Neck Surgery, College of Medicine, Kyung Hee University) ;
  • Cha, Chang-Il (Department of Otorhinolaryngology, Head and Neck Surgery, College of Medicine, Kyung Hee University) ;
  • Park, Dong-Choon (Department of Obstetric and Gynecology, College of Medicine, The Catholic University of Korea)
  • 여승근 (경희대학교 의과대학 이비인후과학교실) ;
  • 차창일 (경희대학교 의과대학 이비인후과학교실) ;
  • 박동춘 (가톨릭대학교 의과대학 산부인과학교실)
  • Published : 2006.09.30
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Abstract

Background: There are at least two different subsets of B cells, B-1 and B-2. The characteristic features and function of B-2 cells in addition to the effect of steroids on B-2 cells are well-known. Although B-1 cells have different features and functions from B-2 cells, the effect of steroids on B-1 cells is not completely understood. Therefore, this study examined the effects of dexamethasone on peritoneal (or B-1 cells) and splenic B cells (or B-2 cells). Methods: Purified B cells were obtained from the peritoneal fluid and the spleens of mice. The isolated B cells were cultured in a medium and after adding different concentrations of dexamaethasone. The cell survival rate was measured by flow cytometry using propidium iodide. The expression level of the B cell surface marker was analyzed by flow cytometry. During the culture of these cells, immunoglobulin secreted into the culture supernatants was evaluated by an enzyme-linked immunosorbent assay. Results: The survival rate of peritoneal and splenic B cells decreased with increasing dexamethasone concentration. However, the rate of peritofieal B cell apoptosis was lower than that of splenic B cells. CDS and B7.1 expression in peritoneal B cells and CD23 and sIgM expression in splenic B cells after the dexamethasone treatment were reduced. When B cells were treated with dexamethasone, the spontaneous IgM secretion decreased with increasing dexamethasone concentration. Conclusion: Dexamethasone induces apoptosis in peritoneal and splenic B cells. However, peritoneal B cells are less sensitive to dexamethasone. The dexamethasone suppressed expression of the surface markers in peritoneal B cells is different from those in splenic B cells.

Keywords

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