Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Effect of Protease on the Extraction and Properties of the Protein from Silkworm pupa

Protease 처리가 누에번데기 단백질 추출 및 기능성에 미치는 영향

  • 권효정 (상주대학교 식품공학과) ;
  • 이경환 (상주대학교 식품공학과) ;
  • 김정환 (엔아이피 바이오텍) ;
  • 천성숙 (영남대학교 식품가공학과) ;
  • 조영제 (상주대학교 식품공학과) ;
  • 차원섭 (상주대학교 식품공학과)
  • Published : 2006.12.31
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Abstract

To extract insoluble proteins from silkworm pupa meal, the meal was treated with pretense produced by Bacillus sp. JH-209. The extraction of insoluble silkworm pupa protein was enhanced at alkaline pHs ranged from 7 to 11 by treatment with the protease. The optimum extraction temperature was $40^{\circ}C$ for in soluble protein treated with pretense. The optimum protease treatment time for extraction of protein was 11 hrs and optimum amount of enzyme treated for extraction of protein was 60 Unit, respectively. The treatment of enzyme extracted more protein than ordinary extraction method without pretense. The foaming capacity, foaming stability, emulsion capacity, and emulsion stability of silkworm pupa meal protein extracted by the treatment of the enzymes increased at all pH ranges. Further more oil absorption as well as water absorption capacities of the protein extracted by the treatment of the enzymes were also increased.

누에번데기에 함유되어 있는 불용성 단백질을 가용성 단백질로 추출시키기 위하여 누에번데기에 Bacillus sp. JH-209 균주로부터 생산된 protease를 작용시켰다. 이때 누에번데기 단백질의 추출을 위한 적정 pH는 pH $7{\sim}11$까지의 알칼리 영역에서 추출율의 증가를 보였다. 최적 온도는 $40^{\circ}C$였고, 최적 작용시간은 11시간이었고, 효소의 최적 첨가량은 60 unit 정도였다. 효소처리 된 누에번데기 단백질은 효소처리하지 않은 대조구에 비해 기포력, 기포안정성, 유화력과 유화안정성이 증가하였고, 유지흡착력과 수분흡착력도 대조구에 비해서 높은값을 나타내었다.

Keywords

References

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