Analysis of Fragrance Volatiles of Korean Rosa hybrida Using Gas Chromatography-Mass Spectrometry

  • Cho, Yong-Sik (Division of Agriproduct Processing, Rural Resources Development Institute, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Cho, In-Hee (Department of Food Science and Technology, College of Engineering, Ehwa Womans University) ;
  • Park, Hong-Ju (Division of Agriproduct Processing, Rural Resources Development Institute, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Chun, Hye-Kyung (Division of Agriproduct Processing, Rural Resources Development Institute, National Institute of Agricultural Science and Technology, Rural Development Administration)
  • Published : 2006.12.31

Abstract

Two different extraction procedures, SDE and SPME, were employed to determine a comprehensive and efficient fragrance profile of Korean Rosa hybrida. Both extraction methods could compensate for each other, covering compounds with diverse boiling point, polarities, and chemical properties. A total of 46 compounds were identified in Mi-hyang. The identified compounds were composed of 17 alcohols, 14 carbonyls, 7 aliphatic hydrocarbons, 2 terpene hydrocarbons, 4 benzenes, 1 ester, and 1 miscellaneous compound. Quantitatively, carbonyls($12.96{\sim}21.79%$ in essential oils of SDE and $2.89{\sim}8.44%$ in SPME headspace) and alcohols($7.98{\sim}11.73%$ in essential oils of SDE and $3.39{\sim}17.35%$ in SPME headspace) were dominant in Mi-hyang's volatiles.

Keywords

References

  1. Rees, J. D. (1991) Reverse enzyme synthesis in microemulsion- based organogels. Biochim. Biophys. Acta 1073, 493-501 https://doi.org/10.1016/0304-4165(91)90221-2
  2. Rajeswara Rao, B. R., Kaul, P. N., Mallavarapu, G. R. and Ramesh, S. (2000) First observation of little leaf disease and its impact on the yield and composition of essential oil of rose-scented geranium (Pelargonium sp.) Flavour Fragr. J. 15, 137-140 https://doi.org/10.1002/1099-1026(200005/06)15:3<137::AID-FFJ880>3.0.CO;2-A
  3. Kim, H. J., Kim, K., Kim, N. S. and Lee, D. S. (2000) Determination of floral fragrances of Rosa hybrida using solid- phase trapping-solvent extraction and gas chromatography- mass spectrometry. J. Chromatogr. A. 902, 389-404 https://doi.org/10.1016/S0021-9673(00)00863-3
  4. Sood, R. P., Singh, B. and Singh, V. J. (1992) Constituents of rose oil from Kangra Valley, H.P. (India). Essent. Oil Res. 4, 425-426 https://doi.org/10.1080/10412905.1992.9698098
  5. Kaul, V. K., Gujral, R. K. and Singh, B. (1999) Volatile constituents of the essential oil flowers of Rosa brunonii Lind. Flavour Fragr. J. 14, 9-11 https://doi.org/10.1002/(SICI)1099-1026(199901/02)14:1<9::AID-FFJ761>3.0.CO;2-C
  6. Knudsen, J. T., Tollsten, L. and Bergstrom, G. (1993) Floral scents-a checklist volatile compounds isolated by headspace techniques. Phytochemistry 33, 253-280 https://doi.org/10.1016/0031-9422(93)85502-I
  7. Dobson, H. E. M., Bergstrom, J., Bergstrom, G. and Groth, I. (1987) Pollen and flower volatiles in two Rosa species. Phytochemistry 26, 3171-3173 https://doi.org/10.1016/S0031-9422(00)82464-4
  8. Cho, I. H., Kim, T. H., Cho, S. K., Lee, H. J. and Kim, Y. S. (2002) Analysis of volatile compounds in bulgogi. Food Sci. Biotechnol. 11, 303-309
  9. van den Dool, H. and Kratz, P. D. (1963) A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. J. Chromatogr. A. 11, 463-471 https://doi.org/10.1016/S0021-9673(01)80947-X
  10. Kondjoyan, N. and Berdague, J. L. (1996) Compilation of relative retention indices for the analysis of aromatic compounds: Laboratoire Faveur, Station de Recherches sur la Viande. Clermont-Ferrand, France
  11. Adams, R. P. (2001) In Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy. Allured Publishing Co., Carol Stream, USA
  12. Yang, X. and Peppard, T. (1999) Food and flavor application, In Solid Phase Microextraction. A Practical Guide, Wercinski SAS. (ed.) pp. 177-201, Marcel Dekker, New York, USA
  13. Siegmund, B. and Pfannhauser, W. Z. (1999) Changes of the volatile fraction of cooked chicken meat during chill storing: Results obtained by the electronic nose in comparison to GC-MS and GC olfactometry. Z. Lebensm. Unters. F. A. 208, 336-341 https://doi.org/10.1007/s002170050426
  14. Kataoka, H., Lord, H. L. and Pawliszyn, J. (2000) Application of solid-phase micro extraction in food analysis. J. Chromatogr. A. 880, 35-62 https://doi.org/10.1016/S0021-9673(00)00309-5
  15. Aydinli, M. and Tutas, M. (2003) Production of rose absolute from rose concrete. Flavour Fragr. J. 18, 26-31 https://doi.org/10.1002/ffj.1138
  16. Rohloff, J. (2002) Volatiles from rhizomes of Rhodiola rosea L. Phytochemistry. 59, 655-661 https://doi.org/10.1016/S0031-9422(02)00004-3