한국균학회지 (The Korean Journal of Mycology)

  • 제34권2호
  • /
  • Pages.112-118
  • /
  • 2006
  • /
  • 0253-651X(pISSN)
  • /
  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
권호 표지
DOI QR코드

DOI QR Code

복숭아혹진딧물 방제를 위한 Lecanicillium 균주 선발

Selection of Lecanicillium Strains for Aphid (Myzus persicae) Control

  • 정혜숙 (수원대학교 생명공학과) ;
  • 이향범 (전남대학교 응용생물공학부) ;
  • 김근 (수원대학교 생명공학과) ;
  • 이은영 (수원대학교 환경공학과)
  • Jung, Hye-Sook (Department of Bioscience & Biotechnology, The University of Suwon) ;
  • Lee, Hyang-Burm (Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Kim, Keun (Department of Bioscience & Biotechnology, The University of Suwon) ;
  • Lee, Eun-Young (Department of Environmental Engineering, The University of Suwon)
  • 발행 : 2006.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 채소류 생산 농가에서 막대한 피해를 주고 있는 해충인 진딧물에 대한 방제효과가 우수한 환경친화적 살충성 미생물 개발을 위한 기초연구로서 살충효과를 갖는 Lecanicilium(Verticillium)속의 우수 균주를 선발하고, 포자생산을 위한 배지를 선정하며, 분자 동정법을 확립하여 새로운 균주 탐색을 위한 기틀을 마련하고자 실시하였다. 새로 동정된 Lecanicillium속 5균주를 이용하여 복숭아혹진딧물(Myzus persicae)에 살충력 시험과 균주들의 여러 특성들을 조사한 결과, 4078 균주는 실온과 $30^{\circ}C$에서 우수한 살충력을 나타냈으며, $30^{\circ}C$에서 2일 만에 100%의 살충성을 보였다. 한편, 낮은 상대 습도에서 가장 우수한 균주는 RH 85%에서 5일 동안 90%의 살충성을 보인 6543 균주이었다. 이들 4078과 6543균주들의 ITS의 DNA 염기서열(accession no.: 순서대로 EF026004와 EF026005)에 의해 Lecanicillium 종으로 확인되었다. 한편, 미생물 농약 생산에 중요한 포자 생산에 있어서 6543 균주가 4078 균주보다 월등히 우수하였는데, 6543 균주의 살충력이 4078 균주보다는 다소 낮지만, 살충력이 여전히 우수하며 포자생산량이 많고, 회수에도 용이하기 때문에, 미생물농약으로서는 6543 균주가 가장 적합하다고 판단된다.

To select efficient entomopathogenic fungal strains of Lecanicillium for the biocontrol of aphid, Myzus persicae, conidial suspension ($1{\times}10\;conidia/ml$) was sprayed onto a detached Chinese cabbage leaf in a petri dish with a dampened filter paper that had 20 nymphs of aphid. Lecancillium strain 4078 and 6543 were the best strains for the biocontrol of aphid at high temperature of $30^{\circ}C$ and low relative humidity (RH) of 85%, respectively. The cumulative mortality of strain 4078 at $30^{\circ}C$ after 3 days was 100% and that of strain 6543 was 90% at 85% RH after 5 days. Strain 4078 also exhibited almost 100% germination ratio of conidia and high rate of mycelial growth at the broad temperature-range of $15{\sim}25^{\circ}C$. The strain 4078 and 6543 were all identified as Lecanicillium species based on the DNA sequences (accession no.: EF026004 and EF026005, respectively) of the ITS regions of the fungi. Excellent production of aerial conidia of strain 6543 was accomplished by using steamed polished rice as the solid culture medium.

키워드

참고문헌

  1. Askary, H., Carriere, Y, Belanger, R. R. and Brodeur, J. 1998. Pathogenecity of the fungus Verticillium lecanii to aphids and powdery mildew. Biocont. Sci. Technol 8: 23-32 https://doi.org/10.1080/09583159830405
  2. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z, Miller, W. and Lipman. D. J. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402 https://doi.org/10.1093/nar/25.17.3389
  3. Feng, M. G, Johnson, J. B. and Kish, L. F. 1990. Virulence of V lecanii and an aphid-derived isolate of Beauveria bassiana (Fungi: Hyphomycetes) for six species of cereal-infesting aphids (Homoptera: Aphididae). Environ. Entomol. 19: 815-820 https://doi.org/10.1093/ee/19.3.815
  4. Gardner, W. A. and McCoy, C. W. 1992. Insecticides and herbicides. In: David B. Finkelstein and Christopher Ball. Biotechnology of Filamentous Fungi. USA: Butterworth-Heinemann. Pp 335-343
  5. Gelemter, W. D. and Evans, H. F. 1999. Overview: Factors in the success and failure of microbial insecticides. Integrated Pest Management Reviews 4: 279 https://doi.org/10.1023/A:1009685908541
  6. Hall, R. A. 1981. The fungus Verticillium lecanii as a microbial insecticide against aphids and scales. Pp 438-498. In: Burges, H. D. Ed. Microbial control of pests and plant diseases 1970-1980. Academic press, London
  7. Hall, R. A. 1984. Epizootic potential for aphids of different isolates of the fungus Verticillium lecanii. Entomophaga 29: 311-321 https://doi.org/10.1007/BF02372119
  8. Hall, R. A. and Burges. H. D. 1979. Control of aphids in the greenhouse with the fungus Verticillium lecanii. Annal. Appl. Biol. 93: 235-246 https://doi.org/10.1111/j.1744-7348.1979.tb06538.x
  9. Jackson, C. W., Heale, J. B. and Hall, R. A. 1985. Traits associated with virulence to the aphid Macrosiphoniella sanborni in eighteen isolates of Verticillium lecanii. Annal. Appl. Biol. 106: 39-48 https://doi.org/10.1111/j.1744-7348.1985.tb03092.x
  10. Kim, J. J. 2004., Pathological studies of Verticillium lecanii on the cotton aphid control. Ph. D. dissertation. Chonnam National University, Korea
  11. Lee, M. H., Yoon, C. S., Yun, T. Y., Kim, H. S. and Yoo, J. K. 2002. Selection of a highly virulent Verticillium lecanii strain against Trialeurodes vaporariorum at various temperatures. J Microbiol. Biotechnol. 12: 145-148
  12. Milner, R. J. 1997, Prospects for biopesticides for aphid control. Entomophaga 42: 227-239 https://doi.org/10.1007/BF02769900
  13. Samuels, K. D. Z., Heale, J. B. and Llewellyn, M. 1989. Characteristics relating to the pathogenicity of Metarhizium anisopliae toward Nilaparvata lugens. J Invert. Pathol. 53: 25-31 https://doi.org/10.1016/0022-2011(89)90070-0
  14. Song, S. S., Oh, H. K. and Motoyama, N. 1993. Insecticide susceptibility of field-collected populations of the spiraea aphids, Aphis citricola (van der Goot) (Homoptera : Aphididae) in apple orchards. Kor. J Appl. Entomol. 32: 259-264
  15. Shah, P. A. and Pell, J. K. 2003. Entomopathogenic fungi as biological control agents. Appl. Microbiol. Biotechnol. 61: 413-423 https://doi.org/10.1007/s00253-003-1240-8
  16. Thompson, J. D., Gibson, T J., Plewniak, F., Jeanmougin, F. and Higgins. D. G 1997. The CLUSTALX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25: 4876-4882 https://doi.org/10.1093/nar/25.24.4876
  17. White, T., Bums, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp 315-322. In: Innis, M. A., Gelfand, D. H., Sninsky, J. J. and White, T. J. Eds. PCR Protocols. Academic press, Sandiego
  18. Yokorni, R. K. and Gottwald, T R. 1988. Virulence of Verticillium lecanii isolates in aphids determined by detached-leaf bioassay. J Invert Pathol. 51: 250-258 https://doi.org/10.1016/0022-2011(88)90032-8
  19. Zoberi, M. H. 1995. Metarhizium anisopliae, fungal pathogen of Reticulitermes flavipes. Mycologia 87: 354-359 https://doi.org/10.2307/3760831

피인용 문헌

  1. Lethal and pathogenic effects ofBeauveria bassianaandLecanicillium lecaniion the adult and nymph ofAphis craccivoraKoch vol.44, pp.1, 2011, https://doi.org/10.1080/03235400902927188
  2. Interaction Between Entomopathogenic Fungi and Some Aphid Species in Thailand vol.65, pp.3, 2013, https://doi.org/10.1007/s10343-013-0302-9
  3. Management of the major piercing sucking pests infesting cucumber under plastic house conditions vol.46, pp.2, 2013, https://doi.org/10.1080/03235408.2012.735543
  4. Selection of Entomopathogenic Fungi for Aphid Control vol.104, pp.6, 2007, https://doi.org/10.1263/jbb.104.498
  5. Effect of entomopathogenic Aspergillus strains against the pea aphid, Acyrthosiphon pisum (Hemiptera: Aphididae) vol.49, pp.3, 2014, https://doi.org/10.1007/s13355-014-0273-z
  6. Pest Control Effect and Optimal dose by Pesticide Dispersion Spray Method in the Paprika Cultivation vol.18, pp.4, 2014, https://doi.org/10.7585/kjps.2014.18.4.350
  7. The Entomopathogenic Fungal Endophytes Purpureocillium lilacinum (Formerly Paecilomyces lilacinus) and Beauveria bassiana Negatively Affect Cotton Aphid Reproduction under Both Greenhouse and Field Conditions vol.9, pp.8, 2014, https://doi.org/10.1371/journal.pone.0103891
  8. Selection of entomopathogenic fungi for aphid control vol.136, 2008, https://doi.org/10.1016/j.jbiotec.2008.07.1955