한국균학회지 (The Korean Journal of Mycology)

  • 제34권2호
  • /
  • Pages.92-97
  • /
  • 2006
  • /
  • 0253-651X(pISSN)
  • /
  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
권호 표지
DOI QR코드

DOI QR Code

구름버섯 균사체 배양 추출물의 복합내성 세균에 대한 항균활성 및 활성물질의 정제

Antimicrobial Activity of the Coriolus versicolor Liquid Culture Extracts Against Antibiotic Resistant Bacteria and Purification of Active Substance

  • 이정선 (가톨릭대학교 생명공학과 & 생물소재공학연구센터) ;
  • 김택 (제주대학교 생명과학과) ;
  • 이윤희 (가톨릭대학교 생명공학과 & 생물소재공학연구센터) ;
  • 김성민 (가톨릭대학교 생명공학과 & 생물소재공학연구센터) ;
  • 김현걸 (가톨릭대학교 생명공학과 & 생물소재공학연구센터) ;
  • 김우중 (가톨릭대학교 생명공학과 & 생물소재공학연구센터) ;
  • 오덕철 (제주대학교 생명과학과) ;
  • 박용일 (가톨릭대학교 생명공학과 & 생물소재공학연구센터)
  • Lee, Jung-Sun (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea) ;
  • Kim, Taeg (Department of Life Science, College of Natural Science, Cheju National University) ;
  • Lee, Yoon-Hi (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea) ;
  • Jin, Cheng-Min (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea) ;
  • Kim, Hyun-Guell (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea) ;
  • Kim, Woo-Jung (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea) ;
  • Oh, Duek-Chul (Department of Life Science, College of Natural Science, Cheju National University) ;
  • Park, Yong-Il (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea)
  • 발행 : 2006.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

구름버섯 균사체를 12% 감귤 추출액에서 7일간 배양한 배양액을 열탕 추출한 후 균사체 잔사를 여과하여 제거하고, 동량의 ethyl acetate(1 : 1, v/v)로 재차 추출하였다. EA 추출물은 복합내성을 갖는 MRSA 균주인 S. aureus CCARM3230와 P. aeruginosa CCARM2171에 대해 현저한 생육저지 활성을 확인하였다. 본 추출물을 silica gel chromatography로 분획하였을 때 dichloromethane : methanol(9 : 1, v/v)의 용매조건으로 용출한 분획물의 항균활성이 가장 높았으며, 활성물질이 높은 순도의 단일 물질로 정제되었음을 HPLC 분석으로 확인하였다. 다제 내성 균주들에 대해 현저한 항균 활성을 보이고, 특히, 그람음성균인 P. aeruginosa CCARM2171과 그람양성균인 S. asures CCARM3230 균주 모두에서 현저한 항균활성을 나타낸 것은 본 추출물의 활성물질이 광범위 항균 스펙트럼을 갖는 신규의 항생물질로 개발될 수 있는 가능성을 보여주는 것으로 사료되었다.

The liquid culture extract of Coriolus versicolor was prepared by directly boiling the whole culture broth 7 days after incubation in 12% citrus extract medium. After removal of mycelial debris through filtration, this extract was further extracted with equal volume of ethyl acetate (1 : 1, v/v). The ethyl acetate extracts showed significant antibacterial activities against Stapylococcus aureus CCARM3230 and Psudomonas aeruginosa CCARM2171, which are resistant to several antibiotics. The most active fraction was eluted from a silica gel column with a mixture of dichloromethane and methanol (9 : 1, v/v) and the purity of this active substance was confirmed by HPLC analysis. The results suggest that the purified active substance could be a good source for the development of a new antimicrobial agent, especially for the treatment of antibiotic resistant bacteria.

키워드

참고문헌

  1. 김용두, 김경제, 조덕봉. 2003. 표고버섯(Lentinus edodes)추출물의 항균활성. 한국식품저장유통학회지 10: 89-93
  2. 박주웅, 김택, 임동중, 이향범, 주이석, 박용일. 2004. 가축질병 세균 및 항생제 내성 세균에 대한 버섯 균사체 배양 추출물의 항균활성. 한국균학회지 32: 145-147 https://doi.org/10.4489/KJM.2004.32.2.145
  3. 이성규. 2003. 가축질병 균주에 대한 소목의 항균활성. 한국미생물생명공학회지 31: 242-249
  4. 이세진, 문성훈, 김택, 김진용, 서정식, 김대선, 김율리아, 김영준, 박용일. 2003. 감귤 농축액에서 배양한 운지버섯 배양추출물의 항산화 및 항암활성. 한국미생물생명공학회지 31:
  5. 이지원, 지영주, 유미희, 임효권, 황보미향, 이인선. 2005. 감초추출물이 항생제 내성균주의 항균활성에 미치는 영향. 한국식품과학회지 37: 456-464
  6. 은재순, 양재헌, 조덕이, 이태규, 박인화. 1989. 한국산 고등균류에 관한 연구(제 2보). 양송이 중의 단백분해효소 활성. 약제학회지 19: 9-14
  7. 최일. 2003. 가축질병 균주에 대한 오배자 추출물의 항균활성. 한국식품영양과학회지 32: 1214-1220
  8. Anke, T., Kupka, J., Schramm, G. and Steglich, W. 1980. Antibiotics from Basidiomycetes X. Scorodonin, a new antibacterial and antifungal metabolites from Marasmius scorodonius (Fr.). J Antibiotics 33: 463-467 https://doi.org/10.7164/antibiotics.33.463
  9. Cambie, R. C., Hirschberg, A., Jones, E. R. J. and Lowe, G 1963. Chemistry of the Higher Fungi Part 14. Polyacetylenic metabolites from Aleurodiscus roseus. J Chem. Soc. 4120-4130 https://doi.org/10.1039/jr9630004120
  10. Cohen, S. H., Morita, M. M. and Bradford, M. 1991. A sevenyear experience with methicillin-resistant Staphylococcus aureus. Am. J Med. 91: 233-237 https://doi.org/10.1016/0002-9343(91)90121-D
  11. Crowford, J. J. 1995. New light on the transmissibility of viral hepatitis in dental practice and its control. JADA 91: 829-835
  12. Francis, A. W. 1995. Staphylococcus aureus (including toxic shock syndrome). Mandell Douglas and Bennetts Principles and Infections Disease. 4: 1754-1755
  13. Han, S. J., Jung, P. M., Kim, H. G., Hwang, E. H. and Seong, I. W. 1999. Multiple intestinal ulcerations and perforations secondary to methicillin-resistant Staphylococcus aureus enteritis in infants. J Pediatr. Surg. 34: 381-386 https://doi.org/10.1016/S0022-3468(99)90481-5
  14. Iinuma, H., Nakamura, H. H., Naganawa, H., Masuda, T, Takano, S., Takeuchi, T., Umezawa, H., Iitaka, Y. and Obayashi, A. 1983. Basidalin, a new antibiotic from Basidomycetes. J Antibiotics 36: 448-450 https://doi.org/10.7164/antibiotics.36.448
  15. Ikekawa, T, Uehara, N., Maeda, Y, Nakamishi, M. and Fukouka, E 1969. Antitumor activity of aqueous extracts of some edible mushroom. Cancer Res. 29: 734-738
  16. Impoolsup, A., Bhumiralana, A. and Flegel, T. 1982. Isolation of alkaline and neutral proteases from Aspergillus flavus var columnaris, a soysauce koji mold. Appl. Environ. Microbiol. 42: 619-625
  17. Kavanagh, F, Ilervey, A. and Robbins, W. J. 1949. Antibiotic substances from Basidomycetes IV. Marasmius conigenus. Proc. Natl. Acad. Sci. 35: 343-351
  18. Kida, T, Shibai, H. and Seto, H. 1986. Structure of new antibiotics, pereniporins A and B from a Basidomycete. J Antibiocics 39: 613-615 https://doi.org/10.7164/antibiotics.39.613
  19. Kielhofner, N., Atmar, R. L, Hanmill, R. J. and Musher, D. M. 1992. Life threatening Pseudomonas aeroginosa infections in patients with human immunodeficiency virus infection. Clin. Infect. Dis. 14: 403-411 https://doi.org/10.1093/clinids/14.2.403
  20. Kupka, J., Anke, T., Oberwinklcr, F, Sohramm, G. and Steglich, W. 1979. Antibiotics from Basidomycetes VII Crinipellin, a new antibiotic from the basidomycetes fungus Crinpellis stipitaria (Fr.). Pat. J Antibiotics. 32: 130-135 https://doi.org/10.7164/antibiotics.32.130
  21. Lockley, R. M., Cohen, M, L., Quinn, T. C., Tompkins, L, S., Coyle, M. B., Kirigara, J. M. and Counts, G. W. 1982. Multiple antibiotic-resistant Staphylococcus aureus: Introduction Transmission and evolution of nosocomial infection. Ann. Interm. Med. 97: 317-324 https://doi.org/10.7326/0003-4819-97-3-317
  22. Midland, S. L., Izac, R. R., Wing, R. M., Zaki, A. I., Munnecke, D. E. and Sims, J. J. 1982. Melleolide, a new antibiotic from Armillaria mella. Tetrahedron Lett. 23: 2515-2518 https://doi.org/10.1016/S0040-4039(00)87383-9
  23. Okamoto, K. A., Shimada, R., Shirai, H., Sakamito, S., Yoshida, F., Olima, Y., Ishiguro, T., Sakai and Kawagishi, H. 1993. Antimicrobial chlorinated orcinol derivatives from mycelia of Hericium erinaceum. Phytochem. 34: 1445-1446 https://doi.org/10.1016/0031-9422(91)80050-B
  24. Park, C. G., Bang, K. H., Lee, S. E., Cha, M. S., Seong, J. S., Park, S. U. and Seong, N. S. 2001. Antimicrobial effect of various medicinal herb on Staphylococcus aureus. Kor. J Medicinal Crop. Sci. 9: 251-258
  25. Piddock, L. J. 1990. Techniques used for the determination of antimicrobial resistance and sensitivity in bacteria. J Appl. Bactriol. 68: 307-318 https://doi.org/10.1111/j.1365-2672.1990.tb02880.x
  26. Troillet, N., Samore, M. H. and Carmeli, Y. 1997. Imipenemresistant Pseudomonas aeroginosa: risk factors and antibiotic susceptibility patterns. Clin. Infect. Dis. 25: 1094-1098 https://doi.org/10.1086/516092
  27. Vogel, F. S., Mcgarry, S. J., Kemyer, L. A. K. and Graham, D. G. 1974. Bacteriological properties of a class of quinoid compound related to sporulation in the mushroom. Agaricus bisporum. Am. J Pathol. 76: 165-174

피인용 문헌

  1. Evaluation of Antioxidant and Antimicrobial Activities of Solvent Extracts from Coriolus versicolor vol.44, pp.12, 2015, https://doi.org/10.3746/jkfn.2015.44.12.1793
  2. Antioxidant Activities and Antimicrobial Effects of Solvent Extracts from Lentinus edodes vol.44, pp.8, 2015, https://doi.org/10.3746/jkfn.2015.44.8.1144
  3. Antimicrobial Effect of the Submerged Culture of Sparassis crispa in Soybean Curd Whey vol.20, pp.1, 2013, https://doi.org/10.11002/kjfp.2013.20.1.111