DOI QR코드

DOI QR Code

Variation in Biochemical Composition among Indian Isolates of Sclerotinia scle-rotiorum

  • Basha S. Ameer (Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University) ;
  • Sarma B.K. (Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University) ;
  • Singh K.P. (College of Forestry & Hill Agriculture, G. B. Pant University of Agriculture and Technology) ;
  • Singh U.P. (Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University)
  • 발행 : 2006.09.30

초록

Biochemical variability among 20 Indian isolates of Sclerotinia sclerotiorum collected from different hosts/soil samples from different localities in India is reported. High Performance Liquid Chromatographic (HPLC) analysis of ethyl acetate fraction of culture filtrate, mycelia, sclerotia and sclerotial exudate showed $15{\sim}23$ peaks but only 11 could be identified. They were tannic, gallic, oxalic, caffeic, vanillic, ferulic, O-coumeric, chlorogenic, cinnamic, salicylic and gentisic acids. The amount of phenolic compounds varied among the culture filtrates, mycelia, sclerotia and sclerotial exudates of S. sclerotiorum.

키워드

참고문헌

  1. Corsini, D. L. and Letourneau, D. 1973. Organic acid metabolism in Sclerotinia sclerotiorum. Arch. Microbiol. 90: 59-64
  2. Chet, I. and Henis, Y. 1975. Sclerotial morphogenesis in fungi. Ann. Rev. Phytopathol. 13: 169-192 https://doi.org/10.1146/annurev.py.13.090175.001125
  3. Cruikshank, R. H. 1983. Distinction between Sclerotinia species by their pectic zymograms. Trans. Brit. Mycol. Soc. 80: 117-119 https://doi.org/10.1016/S0007-1536(83)80171-5
  4. Deng F, Melzer, M. S. and Boland, G. J. 2002. Vegetative compatibility and transmission of hypovirulence - associated dsRNA in Sclerotinia homoeocarpa. Can. J. PI. Pathol. 24: 481-488 https://doi.org/10.1080/07060660209507037
  5. Dutton, M. V. and Evans, C. S. 1996. Oxalate production by fungi: Its role in pathogencity and ecology in the soil environment. Can. J. Microbiol. 42: 881-895 https://doi.org/10.1139/m96-114
  6. Ferror, P. H. and Walker, J. R. L. 1993. O-Diphenol oxidase inhibition - an additional role of oxalic acid in the phytopathogenic arsenal of Sclerotinia sclerotiorum and Sclerotium rolfsii. Physiol. Mol. Pl. Pathol. 43: 415-522 https://doi.org/10.1006/pmpp.1993.1069
  7. Gaffney, T., Friedrich, L., Vemooij, B., Negrotto, D., Nye, G, Uknes, S., Ward, E., Kessmann, H. and Ryals, J. 1993. Requirement of salicylic acid for the induction of systemic acquired resistance. Science 261: 754-756 https://doi.org/10.1126/science.261.5122.754
  8. Godoy, G., Steadman, J. R., Dickman, M. B. and Dam, R. 1990. Use of mutants to demonstrate the role of oxalic acid in pathogenecity of Sclerotinia sclerotiorum on Phaseolus vulgaris. Physiol. Mol. Pl. Pathol. 37: 179-191 https://doi.org/10.1016/0885-5765(90)90010-U
  9. Humpherson-Jones, F. M. and Cook, R. C. 1977. Induction of sclerotium formation by acid staling compounds in Sclerotinia sclerotiorum and Sclerotium rolfsii. Trans. Br. Mycol. Soc. 68: 413-420 https://doi.org/10.1016/S0007-1536(77)80195-2
  10. Keets, S. E., Loewus, F. A., Helms, G. L. and Zink, D. L. 1998. 5-0-(a-D-galactopyranosyl)-D-glycero-pent- 2-enono-1 ,4-lactone : characterization in the oxalate-producing fungus Sclerotinia sclrotiorum. Phytochemistry 49: 2397-2401 https://doi.org/10.1016/S0031-9422(98)00450-6
  11. Kohn, L. M. 1979. A monographic revision of the genus Sclerotinia. Mycotoxon 9: 365-444
  12. Kull, L. S., Pedersen, W. L., Palmquist, D. and Hartman, G. L. 2004. Mycelial compatibility grouping and aggressiveness of Sclerotinia sclerotiorum. Pl. Disease 88: 325-332 https://doi.org/10.1094/PDIS.2004.88.4.325
  13. Magro, P., Marciano, P. and Dilenna, P. 1984. Oxalic acid production and its role in pathogenesis of Sclerotina sclerotiorum (sunflower). FEMS Microbiol. Letters 24: 9-12 https://doi.org/10.1111/j.1574-6968.1984.tb01234.x
  14. Marciano, P., Dilenna, P. and Magro, P. 1983. Oxalic acid, cell wall degrading enzymes and pH in pathogenesis and their significance in the virulence of two Sclerotinia sclerotiorum isolates on sunflower. Physiol. Pl. Pathol 22: 339-345 https://doi.org/10.1016/S0048-4059(83)81021-2
  15. Maxwell, D. P. and Lumsden, R. D. 1970. Oxalic acid production by S. sclerotiorum in infected bean and in culture, Phytopathol. 60: 1395-1398 https://doi.org/10.1094/Phyto-60-1395
  16. Noyes, R. D. and Hanecock, J. G 1981. Role of oxalic acid in the Sclerotinia wilt of Sunflower, Physiol. Pl. Pathol. 18: 123-132 https://doi.org/10.1016/S0048-4059(81)80033-1
  17. Petersen, G. R., Russo, G. M. and Van Etten, J. L. 1982. Identification of major proteins in sclerotia of Sclerotinia minor and Sclerotinia trifoliorum. Exp. Mycol. 6: 268-273 https://doi.org/10.1016/0147-5975(82)90119-0
  18. Purdy, L. H. 1979. Sclerotinia sclerotiorum: history, disease, symptology, host range, geographic distribution and impact. Phytopathol. 69: 875-880 https://doi.org/10.1094/Phyto-69-875
  19. Russo, G. M. and Van Etten, J. L. 1985. Synthesis and localization of development-specific protein in sclerotia in Sclerotinia sclerotiorum. J Bacteriol. 163: 696-703
  20. Sarma, B. K. and Singh, U. P. 2003. Ferulic acid may prevent infection by Sclerotium rolfsii in Cicer arietinum. World. J Microbiol. Biotech. 19: 123-127 https://doi.org/10.1023/A:1023205522032
  21. Scott, S. W. 1981. Serological relationships of three Sclerotinia species. Trans. Brit. Mycol. Soc. 77: 674-676 https://doi.org/10.1016/S0007-1536(81)80129-5
  22. Sharma, S., Harmandeep and Soni, O. 2001. Interaction of phenolic compounds with pectinases from Sclerotinia sclerotiorum.Ind. Phytopathol. 54: 167-170
  23. Singh, U. P., Sarma, B. K., Singh, D. P., Maurya, S., Mishra, P. K. and Singh, H. B. 2004. Effects of exudate depletion on sclerotial development of Sclerotinia sclerotiorum and oxalic acid on the synthesis of phenolic acids in egg plant (Solanum melongena). Z. Pflkrankh. PflSchutz 111: 371-379
  24. Singh, U. P., Sarma, B. K., Singh, D. P., and Amar Bahadur. 2002. Studies on exudate-depleted sclerotial development and effect of oxalic acid, sclerotial exudate and culture filtrate of Sclerotium rolfsii in induction of phenolic acids in chickpea (Cicer arietinum). Can. J Microbiol. 48: 443-448 https://doi.org/10.1139/w02-040
  25. Starratt, A. N., Ross, L. M. and Lazarovits, G 2002. 1,8-Dihydroxynaphthalene monoglucoside, a new metabolite of Sclerotinia sclerotiorum, and the effect of tricyclazole on its production. Can. J Microbiol. 48: 320-325 https://doi.org/10.1139/w02-017
  26. Stephen, G C., Valerie, E. S., Martin, B. D. and Low, P. S. 2000. Oxalic acid, a pathogencity factor for S. sclerotiorum, suppresses the oxidative burst of the host plant. Pl. Cell. 12: 2191-2199 https://doi.org/10.1105/tpc.12.11.2191
  27. Tahmasebi-Enferadi, S., Gomerancher, D., Baladini, M. and Vannozzi, G P. 1998. Effect of Sclerotinia sclerotiorum (Lib.) de Bary culture filter on sunflower morphological characters, oxalic acid content and shikimate dehydrogenase activity.Helia. 21: 81-95
  28. Tariq, V. N., Gutteridge, C. S. and Jeffries, P. 1985. Comparative studies of cultural and biochemical characteristics used for distinguishing species within Sclerotinia. Trans. Brit. Mycol. Soc. 84: 381-397 https://doi.org/10.1016/S0007-1536(85)80001-2
  29. Vega, R. R., Corsini, D. and Le Tourneau, D. 1970. Nonvolatile organic acids produced by Sclerotinia sclerotiorum in synthetic liquid media. Mycologia 62: 332-338 https://doi.org/10.2307/3757591
  30. Wang, S. Y. and Le Tourneau, D. 1972. Amino acids as nitrogen sources for growth and sclerotium formation in Sclerotinia sclerotiorum. Trans. Br. Mycol. Soc. 59: 509-512 https://doi.org/10.1016/S0007-1536(72)80134-7
  31. Willets, H. J. and Wong J. A. L. 1980. The biology of Sclerotinia sclerotiorum, S . trifoliorum and S. minor with emphasis on specific nomenclature. Bot. Rev. 46: 101-165 https://doi.org/10.1007/BF02860868
  32. Willets, H. J. and Bullock, S. 1992. Developmental biology of sclerotia. Mycol. Res. 96: 801-816 https://doi.org/10.1016/S0953-7562(09)81027-7
  33. Zhau Ting, G L. 1999. Mycelial growth and production of oxalic acid by virulent and hypovirulent isolates of Sclerotinia sclerotiorum. Can. J. Pl. Pathol 21: 93-99 https://doi.org/10.1080/07060661.1999.10600090