Maxillofacial Plastic and Reconstructive Surgery

  • 제28권5호
  • /
  • Pages.417-433
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  • 2006
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  • 2288-8101(pISSN)
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  • 2288-8586(eISSN)
대한악안면성형재건외과학회 (Korean Association of Maxillofacial Plastic and Reconstructive Surgeons)
권호 표지

가토 두개골 결손부에 이식된 ${\beta}-TCP$의 골치유 과정에서 PRP의 효과에 관한 연구

THE EFFECT OF PRP ON THE BONE REGENERATION OF ${\beta}-TCP$ GRAFTED IN RABBIT CRANIAL BONE DEFECT

  • 이성훈 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 황경균 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 박창주 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 임병섭 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 조정연 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 백승삼 (한양대학교 의과대학 병리학교실) ;
  • 심광섭 (한양대학교 의과대학 치과학교실 구강악안면외과)
  • Lee, Soung-Hoon (Department of Oral & Maxillofacial Surgery, College of Medicine, Hanyang University) ;
  • Hwang, Kyung-Gyun (Department of Oral & Maxillofacial Surgery, College of Medicine, Hanyang University) ;
  • Park, Chang-Joo (Department of Oral & Maxillofacial Surgery, College of Medicine, Hanyang University) ;
  • Lim, Byung-Sup (Department of Oral & Maxillofacial Surgery, College of Medicine, Hanyang University) ;
  • Cho, Jung-Yeon (Department of Oral & Maxillofacial Surgery, College of Medicine, Hanyang University) ;
  • Paik, Seung-Sam (Department of Pathology, College of Medicine, Hanyang University) ;
  • Shim, Kwang-Sup (Department of Oral & Maxillofacial Surgery, College of Medicine, Hanyang University)
  • 발행 : 2006.09.30
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초록

Purpose : Platelet rich plasma (PRP) is an autologous material with many growth factors, such as BMPs, PDGF, $TGF-{\beta}_1$, $TGF-{\beta}_2$, VEGF, and IGF, facilitating bone healing process. The prominent osteoconductive activity and the biodegradable nature of beta-tricalciumphosphate (${\beta}-TCP$) for bone grafts in animal experiments have been reported. The purpose of this study was to evaluate the effect of PRP on the osteogenesis of ${\beta}-TCP$. Materials & Methods : Two artificial calvarial bone defects were made in 32 rabbits which were divided into 2 groups. In one group of 16 rabbits, autogenous bone / ${\beta}-TCP$ was grafted on each side of cranial bone defect. In the other group of 16 rabbits, mixture of ${\beta}-TCP$ and PRP / PRP alone was grafted on each side of the cranial bone defect. The animals were sacrificed at 2, 4, 8, and 12 weeks after surgery. The specimens were harvested and examined histologically and immunohistochemically by the expression of BMP2/4/7, PDGF, VEGF and $TGF-{\beta}_1$. Results : The mean volume of new bone formation was significantly higher at 4, 8, 12 weeks in autogenous graft than that in ${\beta}-TCP$. The BMP2/4 expression was significantly higher at 4 weeks in autogenous bone graft and at 4 weeks in mixture of ${\beta}-TCP$ and PRP and at 12 weeks in ${\beta}-TCP$. The expression of BMP7, PDGF, VEGF and $TGF-{\beta}_1$ showed no significant difference in autogenous, ${\beta}-TCP$, mixture of ${\beta}-TCP$ and PRP, and PRP alone during grafted bone regeneration. Conclusion : The results showed that PRP had no additional value in promoting healing process of ${\beta}-TCP$ grafts.

키워드

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