International Journal of Oral Biology

  • 제31권1호
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  • Pages.21-26
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  • 2006
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  • 1226-7155(pISSN)
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  • 2287-6618(eISSN)
대한구강생물학회 (The Korean Academy of Oral Biology)
권호 표지

Cyclooxygenase-2 Induction in Porphyromonas gingivalis-Infected THP-1 Monocytic Cells

  • Choi, Eun-Kyoung (Department of Oral Microbiology, Dental Science Research Institute, Chonnam National University Dental School) ;
  • Oh, Byung-Ho (Department of Oral Microbiology, Dental Science Research Institute, Chonnam National University Dental School) ;
  • Kang, In-Chol (Department of Oral Microbiology, Dental Science Research Institute, Chonnam National University Dental School)
  • 발행 : 2006.03.30
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초록

Periodontopathogens including Porphyromonas gingivalis interact with host periodontal cells and the excessive subsequent host responses contribute a major part to the development of periodontal diseases. Cyclooxygenase(COX)-2-synthesized $PGE_2$ has detrimental activities in terms of periodontal pathogenesis. The present study investigated induction of COX-2 expression by P. gingivalis in human monocytic THP-1 cells. Live P. gingivalis increased expression of COX-2, but not that of COX-1, which was demonstrated at both mRNA and protein levels. Elevated levels of $PGE_2$ were released from P. gingivalis-infected THP-1 cells. Pharma-cological inhibition of p38 mitogen-activated protein kinase(MAPK) and extracellular signal-regulated kinase(ERK) substantially attenuated P. gingivalis-induced COX-2 mRNA expression. Indeed, activation of p38 MAPK and ERK was observed in P. gingivalis-infected THP-1 cells. Also, P. gingivalis induced activation of nuclear $factor-{\kappa}B\;(NF-{\kappa}B)$ which is an important transcription factor for COX-2. These results suggest that COX-2 expression is up regulated in P. gingivalis-infected monocytic cells, at least in part, via p38 MAPK, ERK, and $NF-{\kappa}B$.

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참고문헌

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