International Journal of Oral Biology

  • 제31권2호
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  • Pages.45-51
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  • 2006
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  • 1226-7155(pISSN)
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  • 2287-6618(eISSN)
대한구강생물학회 (The Korean Academy of Oral Biology)
권호 표지

Molecular Characterization of $Ca_v2.3$ in Rat Trigeminal Ganglion Neurons

  • Fang, Zhi (Department of Physiology and Program in Molecular and Cellular Neuroscience, College of Dentistry and Dental Research Institute, Seoul National University) ;
  • Kim, Joong-Soo (Department of Physiology and Program in Molecular and Cellular Neuroscience, College of Dentistry and Dental Research Institute, Seoul National University) ;
  • Oh, Seog-Bae (Department of Physiology and Program in Molecular and Cellular Neuroscience, College of Dentistry and Dental Research Institute, Seoul National University)
  • 발행 : 2006.06.30
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초록

R-type($Ca_v2.3$) calcium channel contributes to pain sensation in peripheral sensory neurons. Six isoforms of $Ca_v2.3$ that result from combinations of presence or deletion of three inserts(insert I and insert in the II-III loop, and insert III in N-terminal regions) have been demonstrated to be present in different mammalian tissues. However, the molecular basis of $Ca_v2.3$ in trigeminal ganglion(TG) neurons is not known. In the present study, we determined which isoforms of $Ca_v2.3$ are expressed in rat TG neurons using the RT-PCR analysis. Whole tissue RT-PCR analyses revealed that only two isoforms, $Ca_v2.3a$ and $Ca_v2.3e$, were present in TG neurons. From single-cell RT-PCR, we found that $Ca_v2.3e$ rather than $Ca_v2.3a$ was the major isoform expressed in TG neurons, and $Ca_v2.3e$ was preferentially detected in small-sized neurons that express nociceptive marker, transient receptor potential vanilloid 1(TRPV1). Our results suggest that $Ca_v2.3e$ in trigeminal neurons may be a potential target for the pain treatment.

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