Quality Properties of Appenzeller Cheese Added with Chlorella

클로렐라 첨가 아펜젤러 치즈의 품질 특성

  • Heo, Ji-Youn (Department of Animal Science & Technology, Sunchon National University) ;
  • Shin, Hyun-Jung (R&D Center of Nam Yang Dairy Industry Co. Ltd.) ;
  • Oh, Dong-Hwan (Department of Animal Science & Technology, Sunchon National University) ;
  • Cho, Sung-Kyun (Department of Animal Science & Technology, Sunchon National University) ;
  • Yang, Chul-Ju (Department of Animal Science & Technology, Sunchon National University) ;
  • Kong, Il-Keun (Department of Animal Science & Technology, Sunchon National University) ;
  • Lee, Sang-Suk (Department of Animal Science & Technology, Sunchon National University) ;
  • Choi, Kap-Sung (Faculty of Food Science Sunchon National University) ;
  • Choi, Sung-Hee (Department of Food Science, Sunmoon University) ;
  • Kim, Sang-Chul (Department of Animal Science & Technology, Sunchon National University) ;
  • Choi, Hee-Young (Department of Animal Science & Technology, Sunchon National University) ;
  • Bae, In-Hyu (Department of Animal Science & Technology, Sunchon National University)
  • 허지연 (순천대학교 동물자원과학과) ;
  • 신현정 (남양유업 중앙연구소) ;
  • 오동환 (순천대학교 동물자원과학과) ;
  • 조성균 (순천대학교 동물자원과학과) ;
  • 양철주 (순천대학교 동물자원과학과) ;
  • 공일근 (순천대학교 동물자원과학과) ;
  • 이상석 (순천대학교 동물자원과학과) ;
  • 최갑성 (순천대학교 식품과학부) ;
  • 최성희 (선문대학교 식품과학과) ;
  • 김상철 (순천대학교 동물자원과학과) ;
  • 최희영 (순천대학교 동물자원과학과) ;
  • 배인휴 (순천대학교 동물자원과학과)
  • Published : 2006.12.31

Abstract

Effects of Chlorella powder on the growth of lactic acid bacteria, ripening velocity and organoleptic properties in Appenzeller cheese were investigated. Added levels of Chlorella powder were 0, 0.5, 1.0 and 2.0%. The lactic acid bacteria count was higher in cheese added with Chlorella than those in the control cheese. The pH of cheese increased gradually after 3 weeks, reaching pH $5.4{\sim}6.2$ at 15 weeks of maturation, and the pH was slightly lower in Chlorella added cheese than in control cheese. The soluble nitrogen compounds, non casein nitrogen (NCN) and non protein nitrogen (NPN), in Appenzeller cheese increased during 15 weeks of ripening, and they were higher in Chlorella added cheese than in control cheese. Electrophoresis of cheese proteins revealed that caseins were degraded more rapidly in Chlorella cheese as the level of Chlorella increased so that the cheese with 2% Chlorella could have developed a bitter taste and a stench by an excessive degradation of proteins. Sensory scores of the cheese ripened for 15 weeks were diminished as the level of Chlorella increased especially the cheese added with 2% Chlorella obtained significantly lower values of sensory scores than control cheese. Among the Chlorella cheeses, 0.5% Chlorella added cheese showed the highest score in overall sensory preference. From the results, the adequate level of Chlorella powder being added to produce an Appenzeller cheese product with acceptable quality was suggested to be 0.5%.

본 실험에서는 생리 활성 효과가 기대되며 영양학적 가치가 높은 클로렐라를 실용적인 식재료로 활용하는 방안의 하나로 클로렐라 Appenzeller 치즈를 제조하여 클로렐라 분말이 치즈숙성 중 유산균의 생육과 치즈 pH의 변화 및 단백질의 분해도에 미치는 영향을 살펴보고, 제품의 관능적 특성을 측정하여 제품의 품질을 평가하였다. 대조구보다 클로렐라가 첨가된 치즈에서 유산균수가 다소 높게 유지되었고, pH는 클로렐라 첨가구에서 대체로 더 낮았다. NPN과 NCN은 2.0% 첨가구가 대조구나 다른 첨가구보다 상대적으로 높았다. 조지방과 조회분의 함량은 대조구가 첨가구보다 다소 높았고, 수분은 1.0%에서, 조단백 함량은 2.0%에서 가장 높았다. 클로렐라 분말을 첨가한 치즈들은 관능 평가에서 대조구보다 낮은 점수를 보였으나 1% 이하의 첨가 수준에서는 맛의 변화를 느낄 수 없었으며 특히 2.0% 첨가구에서 가장 낮은 점수가 얻어졌다 Appenzeller 치즈에 대한 클로렐라 분말의 적정 첨가 수준은 치즈의 맛, 외관, 향미 및 조직감 등을 고려했을 때 0.5%수준이 적합한 것으로 사료되었다.

Keywords

References

  1. AOAC (1990) Official methods of analysis, 15th ed, Association Official Analytical Chemists, Washington, DC
  2. Bae, I. H. and Park, J. R. (1994) Proteolytic Characteristics of Tilsiter cheese made with bacteriophage-resistant mutants of Lactococcus lactis ssp. cremoris S10. Foods Biotechnol. 3, 77-82
  3. Bae, I. H. (1988) Stud on properties and caseinolytic action of extracellular protease from Saccharomysopsis lipolytica. Ph. D. thesis, Sungkyunkwan Univ. Seoul, Korea. pp. 78-79
  4. Bae, I. H., Lee, S. W., Kim, G. Y, Nam, M. S., Oh, S. J., and Ha, W. G. (2005) Milk processing technology. Youhan. Publishing Co. Seoul. pp. 215-310
  5. Dantas, D. C. and Queiroz, M. L. (1999) Effects of Chlorella vulgaris on bone marrow progenitor cells of mice infected with Listeria monocytogenes. Int. J. Immunopharmacol. 21, 499-508 https://doi.org/10.1016/S0192-0561(99)00028-4
  6. Hasegawa, T., Ito, K., Veno, S., Kumamoto, S., Ando, Y., Yammada, A., and Nomoto, K. (1999) Oral administration of hot water extracts of Chlorella vulgais reduces IgE production against milk casein in mice. Int. J. Immunopharmacol. 21, 311-323 https://doi.org/10.1016/S0192-0561(99)00013-2
  7. Husain, S. R., Cillard, J., and Cillard, O. (1987) Hydroxyl radical scavenging activity of flavonoids. Phytochemistry 26, 2489-2491 https://doi.org/10.1016/S0031-9422(00)83860-1
  8. Hull, M. E. (1947) Studies on milk protein. II. colorimetric determination of the partial hydrolysis of the proteins in milk. J. Dairy Sci. 30, 881-884 https://doi.org/10.3168/jds.S0022-0302(47)92412-0
  9. Kessler, A., Knusel, H., Raemy, O., Rentsch, F., and Sollberger, H. (1990) Der Tilsiter und der Appenzeller. In: Kiisefabrikation. LMZ-Zollikofen, pp. 71-78
  10. Kim, B. N. (1994) Chlorella. J. of Korean Natural Health 2, 93-107
  11. Kim, C. K. (2002) ChIarella. Dulggotnuri, Seoul. pp. 55-58
  12. Kim, D. H. (1998) Chlorella. Food Journal. 1, 64-69
  13. Kim, H. K., Lee, S. G., Yang, C. Y., Yang, J. B., and Yoon, W. H. (1993) Animal food processing technology. Sejin Publishing Co. Seoul, Korea. pp. 161
  14. Konishi, F., Mitsuyama, M., Okuda, M., Tanaka, K., and Hasegawa, T. (1996) Protective effect of an acidic glycoprotien obtained from culture of Chlorella vulgaris against myelosuppression by 5-fluorouracil. Cancer Immunol. Immunother. 42, 268-274 https://doi.org/10.1007/s002620050281
  15. Korean Society for Cooking Technol. (1998) Cheese & cold cuisine. Heongsul Publishing Co., Seoul, pp. 14-19
  16. Kwak, H. S. (1992) Sensory test of dairy products. Korean Dairy Technol. 10(1), 1-16
  17. Kim, Y. H. (2004) The Function of ChIarella and dairy products. Proceding of 42nd Symposium of Society of Korean Life Science, Busan, Korea. pp. 49-65
  18. Ledford, R. A., Sullivan, A. C., and Nath, K. R. (1966) Residual casein fractions in ripened cheese determined by polyacrylamide-gel electrophoresis. J. Dairy Sci. 49, 1098-1101 https://doi.org/10.3168/jds.S0022-0302(66)88024-4
  19. Mori, A. (1999) Charicteristics and Food value of ChIarella. Food Journal 8, 60-64
  20. Morita, K. and Matsueda, T. (1999) ChIarella accelerates dioxin excretion in rats. J. Nutr. 192, 1731-1736
  21. Murayama, I., Ando, Y., Maeda, T., and Hirayama, K. (1998) Uptake of vitamin Bl2 by various strains of unicellular algae Chlorella. Nippon Suisan Gakkaishi 55, 1785-1790
  22. Nagano, Y., Watanabe, T., Houma, Y., and Suketa, T. (1978) Absorption and excretion of cadmium by the rat administered cadmium-containing Chlorella. Drug Chem. Toxieol. 24, 182-186
  23. Okamoto, K., Lizuka, Y., Muradami, T., Miyake, H., and Suzuki, T. (1978) Effects of Chlorella alkali extracts on blood pressure in SHR. Jpn. Heart J. 19, 622-623 https://doi.org/10.1536/ihj.19.622
  24. Park, S. N., Choi, S. W., Boo, Y. c., Kim, C. K., and Lee, T. Y. (1990) Effects of flavonoids of ginseng leaves on erythrocyte membranes against singlet oxygen caused damage. Korean J. Ginseng Sci. 14, 191-199
  25. Pore, R. S. (1984) Detoxification of chlordecone poisoned rats with Chlorella and Chlorella derived sporopollenin. Drug Chem. Toxieol. 7, 57-71 https://doi.org/10.3109/01480548409014173
  26. Richardson, G. H. (1985) Standard methods for the examination of dairy products. 15th ed, American Public Health Association. Washington DC. pp. 203-218
  27. Sano, T. and Tanaka, Y. (1987) Effect of dried, powdered Chlorella vulgaris on experimental atherosclerosis and alimentary hypercholesterolemia in cholesterol fed rabbits. Artery 14, 76-84
  28. SAS (1996) SAS/STAT Software for PC. Release 6.11, SAS Institute Inc., Cary, NC, USA
  29. Widcombe, R. (1980) The complete book of cheese. Tuttle-Mori Agency, Inc. Tokyo. pp. 26
  30. Yamauchi, K., Kang, K. H., and Kaminogawa, S. (1975) Proteolysis by Debaryornyees hansenii and lactic starters in skim milk culture. Jap. J. Zooteehnol. Sci. 47, 12-17
  31. 姜國煕. 乳蛋白質分解における酵母pと乳酸菌の相互作用に關する硏究. 東京大學大學院博士學位論文. p. 50-52
  32. 福井四朗 (2001) 기적의 체질 혁명 클로렐라. 태응출판사, 서울. pp. 28-37